Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2604 and GFF2605 are separated by 13 nucleotides GFF2605 and GFF2606 are separated by 131 nucleotides GFF2606 and GFF2607 are separated by 11 nucleotides GFF2607 and GFF2608 are separated by 9 nucleotides
GFF2604 - PTS system, galactitol-specific IIC component (EC 2.7.1.69), at 128,823 to 130,136
GFF2604
GFF2605 - Putative nucleoside triphosphatase, at 130,150 to 130,956
GFF2605
GFF2606 - PTS system, IIA component, at 131,088 to 131,525
GFF2606
GFF2607 - Ribulose-phosphate 3-epimerase (EC 5.1.3.1), at 131,537 to 132,169
GFF2607
GFF2608 - Putative arylsulfatase regulatory protein, at 132,179 to 132,976
GFF2608
Position (kb) 131
132 Strain fitness (log2 ratio) -2
-1
0
1 at 130.101 kb on + strand at 130.102 kb on + strand at 130.102 kb on + strand at 130.111 kb on + strand at 130.115 kb on + strand at 130.348 kb on + strand, within GFF2605 at 131.103 kb on - strand at 131.213 kb on + strand, within GFF2606 at 131.214 kb on - strand, within GFF2606 at 131.229 kb on - strand, within GFF2606 at 131.241 kb on + strand, within GFF2606 at 131.242 kb on - strand, within GFF2606 at 131.242 kb on - strand, within GFF2606 at 131.242 kb on - strand, within GFF2606 at 131.321 kb on + strand, within GFF2606 at 131.326 kb on + strand, within GFF2606 at 131.659 kb on + strand, within GFF2607 at 131.666 kb on - strand, within GFF2607 at 131.671 kb on - strand, within GFF2607 at 131.702 kb on + strand, within GFF2607 at 131.840 kb on - strand, within GFF2607 at 132.117 kb on + strand at 132.165 kb on + strand at 132.178 kb on + strand at 132.178 kb on + strand at 132.314 kb on + strand, within GFF2608 at 132.316 kb on + strand, within GFF2608 at 132.317 kb on - strand, within GFF2608 at 132.358 kb on + strand, within GFF2608 at 132.359 kb on - strand, within GFF2608
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 130,101 + -0.1 130,102 + -1.7 130,102 + +0.1 130,111 + -0.1 130,115 + +1.3 130,348 + GFF2605 0.25 -0.1 131,103 - +0.4 131,213 + GFF2606 0.29 -1.2 131,214 - GFF2606 0.29 -1.5 131,229 - GFF2606 0.32 -0.3 131,241 + GFF2606 0.35 -0.9 131,242 - GFF2606 0.35 +0.1 131,242 - GFF2606 0.35 -0.3 131,242 - GFF2606 0.35 -0.2 131,321 + GFF2606 0.53 +1.3 131,326 + GFF2606 0.54 -0.6 131,659 + GFF2607 0.19 -0.4 131,666 - GFF2607 0.20 -0.7 131,671 - GFF2607 0.21 +0.6 131,702 + GFF2607 0.26 -1.1 131,840 - GFF2607 0.48 -1.1 132,117 + +1.1 132,165 + -0.4 132,178 + +0.5 132,178 + +1.0 132,314 + GFF2608 0.17 +0.9 132,316 + GFF2608 0.17 +0.2 132,317 - GFF2608 0.17 -1.8 132,358 + GFF2608 0.22 -0.4 132,359 - GFF2608 0.23 -0.0
Or see this region's nucleotide sequence