Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2562 and GFF2563 are separated by 524 nucleotides GFF2563 and GFF2564 are separated by 83 nucleotides GFF2564 and GFF2565 are separated by 24 nucleotides
GFF2562 - Permease of the drug/metabolite transporter (DMT) superfamily, at 79,441 to 80,265
GFF2562
GFF2563 - Outer membrane porin protein NmpC precursor, at 80,790 to 81,878
GFF2563
GFF2564 - Gfa-like protein, at 81,962 to 82,402
GFF2564
GFF2565 - Methyl viologen resistance protein smvA, at 82,427 to 83,914
GFF2565
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-2
-1
0
1 at 79.827 kb on + strand, within GFF2562 at 79.884 kb on + strand, within GFF2562 at 79.919 kb on - strand, within GFF2562 at 79.979 kb on + strand, within GFF2562 at 80.013 kb on - strand, within GFF2562 at 80.093 kb on + strand, within GFF2562 at 80.113 kb on - strand, within GFF2562 at 80.263 kb on + strand at 80.291 kb on - strand at 80.325 kb on + strand at 80.715 kb on + strand at 80.862 kb on + strand at 80.864 kb on + strand at 80.864 kb on + strand at 80.864 kb on + strand at 80.865 kb on - strand at 80.972 kb on + strand, within GFF2563 at 81.126 kb on + strand, within GFF2563 at 81.233 kb on + strand, within GFF2563 at 81.376 kb on - strand, within GFF2563 at 81.429 kb on - strand, within GFF2563 at 81.669 kb on + strand, within GFF2563 at 81.751 kb on - strand, within GFF2563 at 81.956 kb on + strand at 82.085 kb on + strand, within GFF2564 at 82.086 kb on - strand, within GFF2564 at 82.139 kb on + strand, within GFF2564 at 82.210 kb on + strand, within GFF2564 at 82.232 kb on - strand, within GFF2564 at 82.423 kb on + strand at 82.551 kb on - strand at 82.579 kb on + strand, within GFF2565 at 82.728 kb on + strand, within GFF2565
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 79,827 + GFF2562 0.47 -0.8 79,884 + GFF2562 0.54 +1.3 79,919 - GFF2562 0.58 -0.4 79,979 + GFF2562 0.65 +1.0 80,013 - GFF2562 0.69 -0.2 80,093 + GFF2562 0.79 +0.7 80,113 - GFF2562 0.81 -0.5 80,263 + +0.1 80,291 - -0.1 80,325 + -0.3 80,715 + -0.2 80,862 + +1.2 80,864 + +0.1 80,864 + +0.3 80,864 + +0.2 80,865 - -0.2 80,972 + GFF2563 0.17 -1.7 81,126 + GFF2563 0.31 -0.3 81,233 + GFF2563 0.41 +1.7 81,376 - GFF2563 0.54 +1.3 81,429 - GFF2563 0.59 -2.1 81,669 + GFF2563 0.81 -0.7 81,751 - GFF2563 0.88 -0.6 81,956 + -1.7 82,085 + GFF2564 0.28 -1.5 82,086 - GFF2564 0.28 -1.3 82,139 + GFF2564 0.40 -1.3 82,210 + GFF2564 0.56 -1.0 82,232 - GFF2564 0.61 +0.3 82,423 + -0.5 82,551 - +1.6 82,579 + GFF2565 0.10 -0.1 82,728 + GFF2565 0.20 -0.3
Or see this region's nucleotide sequence