Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF251 and GFF252 are separated by 15 nucleotides GFF252 and GFF253 are separated by 867 nucleotides
GFF251 - Lysophospholipase L2 (EC 3.1.1.5), at 24,694 to 25,710
GFF251
GFF252 - Cof protein, HD superfamily hydrolase, at 25,726 to 26,526
GFF252
GFF253 - Transcriptional activator MetR, at 27,394 to 28,347
GFF253
Position (kb) 25
26
27 Strain fitness (log2 ratio) -1
0
1
2 at 24.892 kb on + strand, within GFF251 at 24.897 kb on - strand, within GFF251 at 24.942 kb on + strand, within GFF251 at 25.093 kb on + strand, within GFF251 at 25.093 kb on + strand, within GFF251 at 25.094 kb on - strand, within GFF251 at 25.095 kb on + strand, within GFF251 at 25.168 kb on - strand, within GFF251 at 25.345 kb on + strand, within GFF251 at 25.591 kb on - strand, within GFF251 at 25.603 kb on + strand, within GFF251 at 25.779 kb on + strand at 25.871 kb on + strand, within GFF252 at 25.872 kb on - strand, within GFF252 at 25.906 kb on + strand, within GFF252 at 25.906 kb on + strand, within GFF252 at 25.962 kb on + strand, within GFF252 at 25.962 kb on + strand, within GFF252 at 26.034 kb on + strand, within GFF252 at 26.035 kb on - strand, within GFF252 at 26.112 kb on + strand, within GFF252 at 26.353 kb on - strand, within GFF252 at 26.520 kb on - strand at 26.541 kb on - strand at 26.572 kb on - strand at 26.593 kb on - strand at 26.624 kb on + strand at 26.625 kb on - strand at 26.630 kb on + strand at 26.657 kb on - strand at 26.687 kb on + strand at 26.691 kb on + strand at 26.692 kb on - strand at 26.692 kb on - strand at 26.692 kb on - strand at 26.768 kb on - strand at 26.784 kb on + strand at 26.785 kb on - strand at 26.785 kb on - strand at 26.786 kb on + strand at 26.787 kb on - strand at 26.820 kb on + strand at 26.820 kb on + strand at 26.821 kb on - strand at 26.821 kb on - strand at 26.821 kb on - strand at 26.823 kb on - strand at 26.841 kb on + strand at 26.841 kb on + strand at 26.841 kb on + strand at 26.841 kb on + strand at 26.842 kb on - strand at 26.895 kb on + strand at 26.897 kb on + strand at 26.911 kb on - strand at 26.955 kb on - strand at 27.047 kb on + strand at 27.048 kb on - strand at 27.048 kb on - strand at 27.050 kb on + strand at 27.079 kb on - strand at 27.262 kb on - strand at 27.266 kb on + strand at 27.267 kb on - strand at 27.276 kb on + strand at 27.312 kb on - strand at 27.320 kb on + strand at 27.321 kb on + strand at 27.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 24,892 + GFF251 0.19 +0.0 24,897 - GFF251 0.20 -0.6 24,942 + GFF251 0.24 +0.2 25,093 + GFF251 0.39 -0.7 25,093 + GFF251 0.39 +2.1 25,094 - GFF251 0.39 +0.6 25,095 + GFF251 0.39 +0.3 25,168 - GFF251 0.47 -0.0 25,345 + GFF251 0.64 +0.4 25,591 - GFF251 0.88 -1.5 25,603 + GFF251 0.89 -0.1 25,779 + -1.6 25,871 + GFF252 0.18 -0.4 25,872 - GFF252 0.18 +0.2 25,906 + GFF252 0.22 -1.1 25,906 + GFF252 0.22 -0.8 25,962 + GFF252 0.29 -0.1 25,962 + GFF252 0.29 +1.4 26,034 + GFF252 0.38 -0.2 26,035 - GFF252 0.39 +0.2 26,112 + GFF252 0.48 -0.5 26,353 - GFF252 0.78 +0.0 26,520 - +0.6 26,541 - -0.3 26,572 - +0.3 26,593 - -1.4 26,624 + +0.0 26,625 - +0.1 26,630 + -0.4 26,657 - +0.5 26,687 + +0.1 26,691 + -0.2 26,692 - +0.1 26,692 - +0.4 26,692 - -0.0 26,768 - +0.2 26,784 + +0.0 26,785 - +0.4 26,785 - +0.1 26,786 + -0.0 26,787 - -0.6 26,820 + -0.7 26,820 + -0.2 26,821 - +0.1 26,821 - -0.1 26,821 - -0.2 26,823 - +0.7 26,841 + -0.3 26,841 + -0.4 26,841 + +0.3 26,841 + +0.1 26,842 - +0.2 26,895 + +0.1 26,897 + -0.2 26,911 - +1.2 26,955 - +0.5 27,047 + -0.0 27,048 - -0.2 27,048 - -0.4 27,050 + +0.5 27,079 - +0.1 27,262 - -0.1 27,266 + -0.1 27,267 - +0.7 27,276 + +0.1 27,312 - +0.3 27,320 + +0.2 27,321 + +2.1 27,396 - +0.3
Or see this region's nucleotide sequence