Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2341 and GFF2342 are separated by 210 nucleotides GFF2342 and GFF2343 are separated by 368 nucleotides
GFF2341 - UPF0141 membrane protein YijP possibly required for phosphoethanolamine modification of lipopolysaccharide, at 132,233 to 133,966
GFF2341
GFF2342 - Phosphoenolpyruvate carboxylase (EC 4.1.1.31), at 134,177 to 136,828
GFF2342
GFF2343 - Acetylornithine deacetylase (EC 3.5.1.16), at 137,197 to 138,348
GFF2343
Position (kb)
134
135
136
137 Strain fitness (log2 ratio)
-1
0
1 at 133.204 kb on - strand, within GFF2341 at 133.214 kb on - strand, within GFF2341 at 133.360 kb on - strand, within GFF2341 at 133.369 kb on + strand, within GFF2341 at 133.372 kb on + strand, within GFF2341 at 133.438 kb on + strand, within GFF2341 at 133.438 kb on + strand, within GFF2341 at 133.439 kb on - strand, within GFF2341 at 133.439 kb on - strand, within GFF2341 at 133.439 kb on - strand, within GFF2341 at 133.439 kb on - strand, within GFF2341 at 133.447 kb on + strand, within GFF2341 at 133.485 kb on + strand, within GFF2341 at 133.529 kb on - strand, within GFF2341 at 133.531 kb on - strand, within GFF2341 at 133.576 kb on - strand, within GFF2341 at 133.576 kb on - strand, within GFF2341 at 133.611 kb on + strand, within GFF2341 at 133.623 kb on + strand, within GFF2341 at 133.697 kb on + strand, within GFF2341 at 133.701 kb on + strand, within GFF2341 at 133.702 kb on - strand, within GFF2341 at 133.704 kb on + strand, within GFF2341 at 133.704 kb on + strand, within GFF2341 at 133.705 kb on - strand, within GFF2341 at 133.705 kb on - strand, within GFF2341 at 133.812 kb on + strand at 133.818 kb on - strand at 133.860 kb on + strand at 133.865 kb on - strand at 133.899 kb on + strand at 133.942 kb on + strand at 133.952 kb on + strand at 133.953 kb on - strand at 133.966 kb on + strand at 134.127 kb on + strand at 134.127 kb on + strand at 134.127 kb on + strand at 134.127 kb on + strand at 134.128 kb on - strand at 134.148 kb on - strand at 134.148 kb on - strand at 134.148 kb on - strand at 134.326 kb on - strand at 134.410 kb on + strand at 134.411 kb on - strand at 134.860 kb on + strand, within GFF2342 at 134.861 kb on - strand, within GFF2342 at 134.864 kb on - strand, within GFF2342 at 135.230 kb on - strand, within GFF2342 at 135.414 kb on + strand, within GFF2342 at 135.653 kb on - strand, within GFF2342 at 135.759 kb on + strand, within GFF2342 at 136.094 kb on - strand, within GFF2342 at 136.100 kb on + strand, within GFF2342 at 136.174 kb on + strand, within GFF2342 at 136.174 kb on + strand, within GFF2342 at 136.174 kb on + strand, within GFF2342 at 136.174 kb on + strand, within GFF2342 at 136.175 kb on - strand, within GFF2342 at 136.175 kb on - strand, within GFF2342 at 136.178 kb on + strand, within GFF2342 at 136.178 kb on + strand, within GFF2342 at 136.267 kb on + strand, within GFF2342 at 136.308 kb on - strand, within GFF2342 at 136.340 kb on - strand, within GFF2342 at 136.340 kb on - strand, within GFF2342 at 136.355 kb on + strand, within GFF2342 at 136.394 kb on - strand, within GFF2342 at 136.451 kb on + strand, within GFF2342 at 136.452 kb on - strand, within GFF2342 at 136.790 kb on - strand at 136.790 kb on - strand at 136.801 kb on + strand at 136.828 kb on + strand at 136.857 kb on - strand at 136.907 kb on - strand at 137.088 kb on + strand at 137.192 kb on - strand at 137.192 kb on - strand at 137.198 kb on + strand at 137.199 kb on - strand at 137.219 kb on - strand at 137.221 kb on + strand at 137.222 kb on - strand at 137.222 kb on - strand at 137.270 kb on + strand at 137.329 kb on + strand, within GFF2343 at 137.330 kb on - strand, within GFF2343 at 137.355 kb on + strand, within GFF2343 at 137.360 kb on + strand, within GFF2343 at 137.455 kb on + strand, within GFF2343 at 137.515 kb on - strand, within GFF2343 at 137.536 kb on + strand, within GFF2343 at 137.797 kb on - strand, within GFF2343 at 137.802 kb on + strand, within GFF2343 at 137.802 kb on + strand, within GFF2343
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 133,204 - GFF2341 0.56 +0.1 133,214 - GFF2341 0.57 +0.2 133,360 - GFF2341 0.65 +0.3 133,369 + GFF2341 0.66 -0.5 133,372 + GFF2341 0.66 -0.7 133,438 + GFF2341 0.69 +0.4 133,438 + GFF2341 0.69 -0.3 133,439 - GFF2341 0.70 -0.6 133,439 - GFF2341 0.70 -0.3 133,439 - GFF2341 0.70 +0.6 133,439 - GFF2341 0.70 +0.1 133,447 + GFF2341 0.70 -0.0 133,485 + GFF2341 0.72 +0.5 133,529 - GFF2341 0.75 +0.3 133,531 - GFF2341 0.75 +1.2 133,576 - GFF2341 0.77 -0.7 133,576 - GFF2341 0.77 -1.0 133,611 + GFF2341 0.79 +0.1 133,623 + GFF2341 0.80 +0.5 133,697 + GFF2341 0.84 -0.1 133,701 + GFF2341 0.85 +0.2 133,702 - GFF2341 0.85 +0.0 133,704 + GFF2341 0.85 +0.1 133,704 + GFF2341 0.85 +0.1 133,705 - GFF2341 0.85 -0.1 133,705 - GFF2341 0.85 +0.6 133,812 + +0.1 133,818 - -0.2 133,860 + -0.1 133,865 - +0.0 133,899 + +0.1 133,942 + -0.5 133,952 + -0.5 133,953 - -0.7 133,966 + +0.1 134,127 + +1.5 134,127 + +0.2 134,127 + -0.3 134,127 + +0.3 134,128 - +0.1 134,148 - -0.2 134,148 - +0.9 134,148 - -0.1 134,326 - +0.0 134,410 + +0.1 134,411 - +0.3 134,860 + GFF2342 0.26 +0.2 134,861 - GFF2342 0.26 +0.3 134,864 - GFF2342 0.26 -0.1 135,230 - GFF2342 0.40 +1.0 135,414 + GFF2342 0.47 +1.4 135,653 - GFF2342 0.56 +0.6 135,759 + GFF2342 0.60 -0.1 136,094 - GFF2342 0.72 -0.0 136,100 + GFF2342 0.73 +0.0 136,174 + GFF2342 0.75 -1.0 136,174 + GFF2342 0.75 -0.3 136,174 + GFF2342 0.75 +1.0 136,174 + GFF2342 0.75 +0.1 136,175 - GFF2342 0.75 +0.6 136,175 - GFF2342 0.75 +0.2 136,178 + GFF2342 0.75 +0.2 136,178 + GFF2342 0.75 +0.2 136,267 + GFF2342 0.79 +0.1 136,308 - GFF2342 0.80 +0.0 136,340 - GFF2342 0.82 +0.4 136,340 - GFF2342 0.82 +0.6 136,355 + GFF2342 0.82 -0.0 136,394 - GFF2342 0.84 -0.4 136,451 + GFF2342 0.86 -0.2 136,452 - GFF2342 0.86 -0.6 136,790 - -0.4 136,790 - -1.6 136,801 + -0.3 136,828 + -0.8 136,857 - -0.4 136,907 - -0.1 137,088 + +0.0 137,192 - -0.1 137,192 - -0.7 137,198 + -0.3 137,199 - -0.3 137,219 - -0.3 137,221 + -0.4 137,222 - -0.7 137,222 - +0.6 137,270 + -0.7 137,329 + GFF2343 0.11 +0.6 137,330 - GFF2343 0.12 +0.4 137,355 + GFF2343 0.14 -0.2 137,360 + GFF2343 0.14 +0.9 137,455 + GFF2343 0.22 +1.0 137,515 - GFF2343 0.28 -0.1 137,536 + GFF2343 0.29 +0.0 137,797 - GFF2343 0.52 +0.6 137,802 + GFF2343 0.53 -1.0 137,802 + GFF2343 0.53 -0.6
Or see this region's nucleotide sequence