Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1948 and GFF1949 overlap by 1 nucleotides GFF1949 and GFF1950 are separated by 3 nucleotides GFF1950 and GFF1951 are separated by 35 nucleotides
GFF1948 - Putative membrane protein YfcA, at 139,294 to 140,103
GFF1948
GFF1949 - Murein endopeptidase, at 140,103 to 140,927
GFF1949
GFF1950 - Chorismate synthase (EC 4.2.3.5), at 140,931 to 142,016
GFF1950
GFF1951 - Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p, at 142,052 to 142,984
GFF1951
Position (kb) 140
141
142
143 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 140.014 kb on - strand, within GFF1948 at 140.080 kb on - strand at 140.080 kb on - strand at 140.110 kb on - strand at 140.244 kb on + strand, within GFF1949 at 140.301 kb on - strand, within GFF1949 at 140.333 kb on + strand, within GFF1949 at 140.334 kb on - strand, within GFF1949 at 140.381 kb on + strand, within GFF1949 at 140.524 kb on - strand, within GFF1949 at 140.851 kb on - strand at 141.135 kb on - strand, within GFF1950 at 141.685 kb on + strand, within GFF1950 at 141.686 kb on - strand, within GFF1950 at 141.686 kb on - strand, within GFF1950 at 141.851 kb on + strand, within GFF1950 at 141.942 kb on - strand at 142.063 kb on - strand at 142.185 kb on - strand, within GFF1951 at 142.234 kb on - strand, within GFF1951 at 142.356 kb on + strand, within GFF1951 at 142.367 kb on - strand, within GFF1951 at 142.384 kb on - strand, within GFF1951 at 142.441 kb on + strand, within GFF1951 at 142.462 kb on + strand, within GFF1951 at 142.530 kb on + strand, within GFF1951 at 142.531 kb on - strand, within GFF1951 at 142.824 kb on + strand, within GFF1951 at 142.942 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 140,014 - GFF1948 0.89 +0.0 140,080 - -0.2 140,080 - +0.5 140,110 - -0.2 140,244 + GFF1949 0.17 -0.8 140,301 - GFF1949 0.24 +1.2 140,333 + GFF1949 0.28 +2.8 140,334 - GFF1949 0.28 +0.3 140,381 + GFF1949 0.34 +0.1 140,524 - GFF1949 0.51 -1.7 140,851 - -0.5 141,135 - GFF1950 0.19 -0.4 141,685 + GFF1950 0.69 +1.0 141,686 - GFF1950 0.70 -1.1 141,686 - GFF1950 0.70 -0.5 141,851 + GFF1950 0.85 -2.3 141,942 - +1.3 142,063 - +0.7 142,185 - GFF1951 0.14 -1.0 142,234 - GFF1951 0.20 +0.2 142,356 + GFF1951 0.33 -1.7 142,367 - GFF1951 0.34 +0.3 142,384 - GFF1951 0.36 +0.8 142,441 + GFF1951 0.42 -0.5 142,462 + GFF1951 0.44 -0.6 142,530 + GFF1951 0.51 +0.8 142,531 - GFF1951 0.51 +0.6 142,824 + GFF1951 0.83 -0.0 142,942 + +0.8
Or see this region's nucleotide sequence