Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1766 and GFF1767 are separated by 25 nucleotides GFF1767 and GFF1768 overlap by 14 nucleotides GFF1768 and GFF1769 are separated by 24 nucleotides
GFF1766 - D-galactonate transporter, at 69,329 to 70,627
GFF1766
GFF1767 - Sensor protein torS (EC 2.7.3.-), at 70,653 to 73,340
GFF1767
GFF1768 - hypothetical protein, at 73,327 to 73,443
GFF1768
GFF1769 - Periplasmic protein torT precursor, at 73,468 to 74,508
GFF1769
Position (kb)
70
71
72
73
74 Strain fitness (log2 ratio)
-1
0
1 at 69.724 kb on + strand, within GFF1766 at 69.768 kb on - strand, within GFF1766 at 69.878 kb on - strand, within GFF1766 at 69.934 kb on + strand, within GFF1766 at 69.953 kb on + strand, within GFF1766 at 69.953 kb on + strand, within GFF1766 at 69.954 kb on - strand, within GFF1766 at 70.054 kb on + strand, within GFF1766 at 70.055 kb on - strand, within GFF1766 at 70.096 kb on + strand, within GFF1766 at 70.127 kb on - strand, within GFF1766 at 70.186 kb on - strand, within GFF1766 at 70.213 kb on - strand, within GFF1766 at 70.288 kb on + strand, within GFF1766 at 70.354 kb on + strand, within GFF1766 at 70.402 kb on + strand, within GFF1766 at 70.403 kb on - strand, within GFF1766 at 70.403 kb on - strand, within GFF1766 at 70.492 kb on + strand, within GFF1766 at 70.554 kb on - strand at 70.554 kb on - strand at 70.654 kb on + strand at 70.655 kb on - strand at 70.712 kb on + strand at 70.718 kb on + strand at 70.719 kb on - strand at 70.722 kb on - strand at 70.725 kb on + strand at 70.725 kb on + strand at 70.827 kb on - strand at 71.093 kb on + strand, within GFF1767 at 71.094 kb on - strand, within GFF1767 at 71.307 kb on - strand, within GFF1767 at 71.310 kb on - strand, within GFF1767 at 71.310 kb on - strand, within GFF1767 at 71.310 kb on - strand, within GFF1767 at 71.325 kb on - strand, within GFF1767 at 71.328 kb on - strand, within GFF1767 at 71.388 kb on - strand, within GFF1767 at 71.585 kb on + strand, within GFF1767 at 71.613 kb on - strand, within GFF1767 at 71.649 kb on + strand, within GFF1767 at 71.681 kb on + strand, within GFF1767 at 71.681 kb on + strand, within GFF1767 at 71.682 kb on - strand, within GFF1767 at 71.731 kb on - strand, within GFF1767 at 71.808 kb on - strand, within GFF1767 at 71.892 kb on - strand, within GFF1767 at 71.930 kb on + strand, within GFF1767 at 72.219 kb on - strand, within GFF1767 at 72.354 kb on + strand, within GFF1767 at 72.379 kb on - strand, within GFF1767 at 72.388 kb on - strand, within GFF1767 at 72.388 kb on - strand, within GFF1767 at 72.419 kb on + strand, within GFF1767 at 72.420 kb on - strand, within GFF1767 at 72.478 kb on + strand, within GFF1767 at 72.581 kb on - strand, within GFF1767 at 72.678 kb on - strand, within GFF1767 at 72.726 kb on + strand, within GFF1767 at 72.727 kb on - strand, within GFF1767 at 72.906 kb on - strand, within GFF1767 at 72.967 kb on - strand, within GFF1767 at 73.390 kb on + strand, within GFF1768 at 73.515 kb on + strand at 73.518 kb on - strand at 73.547 kb on + strand at 73.547 kb on + strand at 73.629 kb on - strand, within GFF1769 at 73.652 kb on - strand, within GFF1769 at 73.652 kb on - strand, within GFF1769 at 73.813 kb on - strand, within GFF1769 at 73.840 kb on + strand, within GFF1769 at 73.841 kb on - strand, within GFF1769 at 73.880 kb on + strand, within GFF1769 at 73.934 kb on - strand, within GFF1769 at 74.055 kb on + strand, within GFF1769 at 74.211 kb on + strand, within GFF1769
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 69,724 + GFF1766 0.30 +0.8 69,768 - GFF1766 0.34 +0.1 69,878 - GFF1766 0.42 -0.1 69,934 + GFF1766 0.47 +0.1 69,953 + GFF1766 0.48 -0.1 69,953 + GFF1766 0.48 +0.1 69,954 - GFF1766 0.48 +0.1 70,054 + GFF1766 0.56 -0.3 70,055 - GFF1766 0.56 +0.3 70,096 + GFF1766 0.59 -0.0 70,127 - GFF1766 0.61 +0.0 70,186 - GFF1766 0.66 +0.1 70,213 - GFF1766 0.68 +0.2 70,288 + GFF1766 0.74 -0.0 70,354 + GFF1766 0.79 +1.4 70,402 + GFF1766 0.83 -0.1 70,403 - GFF1766 0.83 -0.2 70,403 - GFF1766 0.83 -0.7 70,492 + GFF1766 0.90 -1.1 70,554 - -0.1 70,554 - -0.4 70,654 + +0.1 70,655 - -0.0 70,712 + +0.2 70,718 + +0.1 70,719 - -0.5 70,722 - -0.0 70,725 + +0.3 70,725 + -0.6 70,827 - +0.5 71,093 + GFF1767 0.16 -0.1 71,094 - GFF1767 0.16 +0.4 71,307 - GFF1767 0.24 -0.5 71,310 - GFF1767 0.24 -0.5 71,310 - GFF1767 0.24 +0.2 71,310 - GFF1767 0.24 +0.1 71,325 - GFF1767 0.25 -0.9 71,328 - GFF1767 0.25 +0.1 71,388 - GFF1767 0.27 +1.1 71,585 + GFF1767 0.35 -1.7 71,613 - GFF1767 0.36 -0.1 71,649 + GFF1767 0.37 +0.3 71,681 + GFF1767 0.38 +0.4 71,681 + GFF1767 0.38 -0.1 71,682 - GFF1767 0.38 +0.8 71,731 - GFF1767 0.40 +0.5 71,808 - GFF1767 0.43 -0.3 71,892 - GFF1767 0.46 +0.8 71,930 + GFF1767 0.48 +0.3 72,219 - GFF1767 0.58 -0.2 72,354 + GFF1767 0.63 +0.3 72,379 - GFF1767 0.64 -0.3 72,388 - GFF1767 0.65 -0.0 72,388 - GFF1767 0.65 +0.4 72,419 + GFF1767 0.66 -0.3 72,420 - GFF1767 0.66 +0.6 72,478 + GFF1767 0.68 -0.1 72,581 - GFF1767 0.72 -0.5 72,678 - GFF1767 0.75 -0.1 72,726 + GFF1767 0.77 +0.4 72,727 - GFF1767 0.77 +1.3 72,906 - GFF1767 0.84 -0.9 72,967 - GFF1767 0.86 +0.8 73,390 + GFF1768 0.54 -1.4 73,515 + +0.3 73,518 - +0.5 73,547 + +0.6 73,547 + -0.6 73,629 - GFF1769 0.15 +0.5 73,652 - GFF1769 0.18 -0.2 73,652 - GFF1769 0.18 -0.3 73,813 - GFF1769 0.33 +0.5 73,840 + GFF1769 0.36 +0.7 73,841 - GFF1769 0.36 +0.0 73,880 + GFF1769 0.40 -0.1 73,934 - GFF1769 0.45 +0.2 74,055 + GFF1769 0.56 -0.4 74,211 + GFF1769 0.71 -0.2
Or see this region's nucleotide sequence