Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1304 and GFF1305 overlap by 16 nucleotides GFF1305 and GFF1306 overlap by 4 nucleotides GFF1306 and GFF1307 are separated by 29 nucleotides GFF1307 and GFF1308 are separated by 48 nucleotides GFF1308 and GFF1309 overlap by 8 nucleotides GFF1309 and GFF1310 are separated by 6 nucleotides
GFF1304 - FIG004136: Prepilin peptidase dependent protein C precursor, at 14,907 to 15,227
GFF1304
GFF1305 - FIG006270: hypothetical protein, at 15,212 to 15,619
GFF1305
GFF1306 - FIG004819: Prepilin peptidase dependent protein B precursor, at 15,616 to 16,140
GFF1306
GFF1307 - Prepilin peptidase dependent protein A precursor, at 16,170 to 16,640
GFF1307
GFF1308 - FIG102772: hypothetical protein, at 16,689 to 16,832
GFF1308
GFF1309 - Thymidylate synthase (EC 2.1.1.45), at 16,825 to 17,619
GFF1309
GFF1310 - Prolipoprotein diacylglyceryl transferase (EC 2.4.99.-), at 17,626 to 18,501
GFF1310
Position (kb) 16
17 Strain fitness (log2 ratio) -2
-1
0
1 at 15.400 kb on - strand, within GFF1305 at 15.434 kb on + strand, within GFF1305 at 15.434 kb on + strand, within GFF1305 at 15.435 kb on - strand, within GFF1305 at 15.485 kb on + strand, within GFF1305 at 15.520 kb on - strand, within GFF1305 at 15.540 kb on - strand, within GFF1305 at 15.552 kb on - strand, within GFF1305 at 15.572 kb on + strand, within GFF1305 at 15.587 kb on + strand at 15.591 kb on - strand at 15.741 kb on + strand, within GFF1306 at 15.757 kb on + strand, within GFF1306 at 15.882 kb on + strand, within GFF1306 at 15.901 kb on - strand, within GFF1306 at 16.012 kb on - strand, within GFF1306 at 16.013 kb on + strand, within GFF1306 at 16.013 kb on + strand, within GFF1306 at 16.014 kb on - strand, within GFF1306 at 16.014 kb on - strand, within GFF1306 at 16.078 kb on + strand, within GFF1306 at 16.323 kb on - strand, within GFF1307 at 16.395 kb on - strand, within GFF1307 at 16.619 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 15,400 - GFF1305 0.46 +0.7 15,434 + GFF1305 0.54 -0.1 15,434 + GFF1305 0.54 +1.0 15,435 - GFF1305 0.55 +0.5 15,485 + GFF1305 0.67 +0.2 15,520 - GFF1305 0.75 +0.0 15,540 - GFF1305 0.80 -0.7 15,552 - GFF1305 0.83 -0.7 15,572 + GFF1305 0.88 -0.1 15,587 + -0.1 15,591 - +1.3 15,741 + GFF1306 0.24 +0.2 15,757 + GFF1306 0.27 -0.0 15,882 + GFF1306 0.51 -0.3 15,901 - GFF1306 0.54 +0.2 16,012 - GFF1306 0.75 +0.1 16,013 + GFF1306 0.76 -0.6 16,013 + GFF1306 0.76 -0.3 16,014 - GFF1306 0.76 -2.1 16,014 - GFF1306 0.76 +0.9 16,078 + GFF1306 0.88 -0.3 16,323 - GFF1307 0.32 +0.2 16,395 - GFF1307 0.48 -0.6 16,619 + -0.5
Or see this region's nucleotide sequence