Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1209 and GFF1210 are separated by 240 nucleotides GFF1210 and GFF1211 are separated by 14 nucleotides GFF1211 and GFF1212 overlap by 8 nucleotides
GFF1209 - putative outer membrane protein, at 48,771 to 49,658
GFF1209
GFF1210 - Kappa-fimbriae probable subunit, at 49,899 to 50,456
GFF1210
GFF1211 - Kappa-fimbriae chaperone protein, at 50,471 to 51,163
GFF1211
GFF1212 - Kappa-fimbriae usher protein, at 51,156 to 53,564
GFF1212
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 49.477 kb on - strand, within GFF1209 at 49.482 kb on - strand, within GFF1209 at 49.512 kb on - strand, within GFF1209 at 49.517 kb on + strand, within GFF1209 at 49.540 kb on + strand, within GFF1209 at 49.540 kb on + strand, within GFF1209 at 49.553 kb on + strand, within GFF1209 at 49.553 kb on + strand, within GFF1209 at 49.554 kb on - strand, within GFF1209 at 49.555 kb on + strand, within GFF1209 at 49.555 kb on + strand, within GFF1209 at 49.556 kb on - strand, within GFF1209 at 49.556 kb on - strand, within GFF1209 at 49.556 kb on - strand, within GFF1209 at 49.563 kb on + strand, within GFF1209 at 49.564 kb on - strand, within GFF1209 at 49.642 kb on - strand at 49.672 kb on + strand at 49.679 kb on + strand at 49.679 kb on + strand at 49.680 kb on - strand at 49.683 kb on + strand at 49.683 kb on + strand at 49.683 kb on + strand at 49.683 kb on + strand at 49.684 kb on - strand at 49.704 kb on - strand at 49.715 kb on + strand at 49.723 kb on + strand at 49.724 kb on - strand at 49.724 kb on - strand at 49.728 kb on + strand at 49.729 kb on - strand at 49.740 kb on + strand at 49.793 kb on + strand at 49.852 kb on + strand at 49.859 kb on + strand at 50.010 kb on + strand, within GFF1210 at 50.054 kb on + strand, within GFF1210 at 50.055 kb on - strand, within GFF1210 at 50.122 kb on - strand, within GFF1210 at 50.192 kb on + strand, within GFF1210 at 50.193 kb on - strand, within GFF1210 at 50.269 kb on + strand, within GFF1210 at 50.270 kb on - strand, within GFF1210 at 50.279 kb on + strand, within GFF1210 at 50.280 kb on - strand, within GFF1210 at 50.283 kb on - strand, within GFF1210 at 50.349 kb on - strand, within GFF1210 at 50.485 kb on - strand at 50.485 kb on - strand at 50.930 kb on - strand, within GFF1211 at 50.955 kb on + strand, within GFF1211 at 50.955 kb on + strand, within GFF1211 at 50.955 kb on + strand, within GFF1211 at 50.956 kb on - strand, within GFF1211 at 50.956 kb on - strand, within GFF1211 at 50.956 kb on - strand, within GFF1211 at 50.956 kb on - strand, within GFF1211 at 50.956 kb on - strand, within GFF1211 at 51.064 kb on - strand, within GFF1211 at 51.157 kb on + strand at 51.157 kb on + strand at 51.158 kb on - strand at 51.158 kb on - strand at 51.166 kb on + strand at 51.192 kb on - strand at 51.201 kb on - strand at 51.201 kb on - strand at 51.272 kb on + strand at 51.273 kb on - strand at 51.273 kb on - strand at 51.385 kb on + strand at 51.521 kb on - strand, within GFF1212 at 51.545 kb on - strand, within GFF1212 at 51.545 kb on - strand, within GFF1212 at 51.545 kb on - strand, within GFF1212 at 51.704 kb on + strand, within GFF1212 at 51.901 kb on + strand, within GFF1212 at 51.902 kb on - strand, within GFF1212 at 51.935 kb on + strand, within GFF1212 at 51.955 kb on + strand, within GFF1212 at 51.974 kb on - strand, within GFF1212 at 51.983 kb on + strand, within GFF1212 at 51.984 kb on - strand, within GFF1212 at 51.988 kb on + strand, within GFF1212 at 51.988 kb on + strand, within GFF1212 at 51.988 kb on + strand, within GFF1212 at 51.989 kb on - strand, within GFF1212 at 51.989 kb on - strand, within GFF1212 at 52.032 kb on - strand, within GFF1212 at 52.078 kb on + strand, within GFF1212 at 52.079 kb on - strand, within GFF1212 at 52.079 kb on - strand, within GFF1212 at 52.109 kb on + strand, within GFF1212 at 52.162 kb on + strand, within GFF1212
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 49,477 - GFF1209 0.80 -0.1 49,482 - GFF1209 0.80 -0.4 49,512 - GFF1209 0.83 -0.4 49,517 + GFF1209 0.84 +0.1 49,540 + GFF1209 0.87 +0.1 49,540 + GFF1209 0.87 +0.1 49,553 + GFF1209 0.88 +0.4 49,553 + GFF1209 0.88 +0.1 49,554 - GFF1209 0.88 +0.1 49,555 + GFF1209 0.88 -1.5 49,555 + GFF1209 0.88 +0.0 49,556 - GFF1209 0.88 -0.0 49,556 - GFF1209 0.88 -0.1 49,556 - GFF1209 0.88 -0.6 49,563 + GFF1209 0.89 -0.1 49,564 - GFF1209 0.89 -0.7 49,642 - +0.3 49,672 + +0.0 49,679 + -0.6 49,679 + -1.2 49,680 - -0.1 49,683 + +0.6 49,683 + +0.8 49,683 + -0.3 49,683 + -0.1 49,684 - +0.1 49,704 - +0.6 49,715 + -0.7 49,723 + +0.1 49,724 - -0.3 49,724 - +0.2 49,728 + -0.3 49,729 - -0.5 49,740 + -1.0 49,793 + -0.3 49,852 + +0.1 49,859 + -0.0 50,010 + GFF1210 0.20 +0.5 50,054 + GFF1210 0.28 +0.0 50,055 - GFF1210 0.28 +0.1 50,122 - GFF1210 0.40 +0.4 50,192 + GFF1210 0.53 -0.7 50,193 - GFF1210 0.53 +0.1 50,269 + GFF1210 0.66 +1.7 50,270 - GFF1210 0.66 +1.1 50,279 + GFF1210 0.68 -0.2 50,280 - GFF1210 0.68 +0.4 50,283 - GFF1210 0.69 +0.1 50,349 - GFF1210 0.81 +0.4 50,485 - +2.5 50,485 - -0.8 50,930 - GFF1211 0.66 -1.1 50,955 + GFF1211 0.70 -0.1 50,955 + GFF1211 0.70 -0.1 50,955 + GFF1211 0.70 +0.4 50,956 - GFF1211 0.70 -0.0 50,956 - GFF1211 0.70 +1.0 50,956 - GFF1211 0.70 -0.0 50,956 - GFF1211 0.70 -0.2 50,956 - GFF1211 0.70 +0.7 51,064 - GFF1211 0.86 +0.7 51,157 + -0.2 51,157 + +0.1 51,158 - -0.5 51,158 - -0.3 51,166 + -0.5 51,192 - -0.4 51,201 - +0.3 51,201 - +0.4 51,272 + -0.2 51,273 - -0.0 51,273 - +0.2 51,385 + +0.2 51,521 - GFF1212 0.15 -0.1 51,545 - GFF1212 0.16 -0.2 51,545 - GFF1212 0.16 -0.0 51,545 - GFF1212 0.16 +0.5 51,704 + GFF1212 0.23 -0.4 51,901 + GFF1212 0.31 -0.1 51,902 - GFF1212 0.31 +0.5 51,935 + GFF1212 0.32 +1.1 51,955 + GFF1212 0.33 -0.9 51,974 - GFF1212 0.34 -0.4 51,983 + GFF1212 0.34 -0.5 51,984 - GFF1212 0.34 -0.4 51,988 + GFF1212 0.35 -0.7 51,988 + GFF1212 0.35 -0.1 51,988 + GFF1212 0.35 +0.4 51,989 - GFF1212 0.35 -2.4 51,989 - GFF1212 0.35 -0.4 52,032 - GFF1212 0.36 -1.6 52,078 + GFF1212 0.38 +0.8 52,079 - GFF1212 0.38 -0.0 52,079 - GFF1212 0.38 +0.4 52,109 + GFF1212 0.40 -1.0 52,162 + GFF1212 0.42 -0.2
Or see this region's nucleotide sequence