Experiment: LB_plus_SM_buffer with 6F2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1194 and GFF1195 are separated by 581 nucleotides GFF1195 and GFF1196 are separated by 56 nucleotides GFF1196 and GFF1197 are separated by 48 nucleotides GFF1197 and GFF1198 are separated by 96 nucleotides
GFF1194 - DNA replication protein, at 42,131 to 43,012
GFF1194
GFF1195 - hypothetical protein, at 43,594 to 43,716
GFF1195
GFF1196 - hypothetical protein, at 43,773 to 43,904
GFF1196
GFF1197 - Outer membrane protein assembly factor YaeT precursor, at 43,953 to 44,093
GFF1197
GFF1198 - Porin thermoregulatory protein envY, at 44,190 to 44,948
GFF1198
Position (kb) 43
44 Strain fitness (log2 ratio) -2
-1
0
1
2 at 42.771 kb on - strand, within GFF1194 at 43.009 kb on - strand at 43.010 kb on + strand at 43.011 kb on - strand at 43.115 kb on + strand at 43.334 kb on + strand at 43.428 kb on + strand at 43.428 kb on + strand at 43.428 kb on + strand at 43.428 kb on + strand at 43.429 kb on - strand at 43.432 kb on - strand at 43.661 kb on + strand, within GFF1195 at 43.661 kb on + strand, within GFF1195 at 43.662 kb on - strand, within GFF1195 at 43.662 kb on - strand, within GFF1195 at 43.787 kb on + strand, within GFF1196 at 43.787 kb on + strand, within GFF1196 at 43.787 kb on + strand, within GFF1196 at 43.788 kb on - strand, within GFF1196 at 43.788 kb on - strand, within GFF1196 at 43.788 kb on - strand, within GFF1196 at 43.817 kb on - strand, within GFF1196 at 43.817 kb on - strand, within GFF1196 at 43.817 kb on - strand, within GFF1196 at 43.842 kb on - strand, within GFF1196 at 43.853 kb on - strand, within GFF1196 at 43.887 kb on + strand, within GFF1196 at 44.135 kb on - strand at 44.173 kb on - strand at 44.175 kb on - strand at 44.186 kb on - strand at 44.281 kb on + strand, within GFF1198 at 44.292 kb on - strand, within GFF1198 at 44.308 kb on + strand, within GFF1198 at 44.384 kb on + strand, within GFF1198 at 44.385 kb on - strand, within GFF1198 at 44.392 kb on + strand, within GFF1198 at 44.393 kb on - strand, within GFF1198 at 44.393 kb on - strand, within GFF1198 at 44.393 kb on - strand, within GFF1198 at 44.443 kb on + strand, within GFF1198 at 44.443 kb on + strand, within GFF1198 at 44.467 kb on - strand, within GFF1198 at 44.496 kb on + strand, within GFF1198 at 44.523 kb on - strand, within GFF1198 at 44.630 kb on - strand, within GFF1198 at 44.630 kb on - strand, within GFF1198
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6F2_phage 15 MOI remove 42,771 - GFF1194 0.73 -0.6 43,009 - -0.1 43,010 + +0.2 43,011 - +0.4 43,115 + -0.6 43,334 + +0.3 43,428 + +1.0 43,428 + -0.1 43,428 + -0.4 43,428 + -0.3 43,429 - +1.1 43,432 - -0.4 43,661 + GFF1195 0.54 -0.1 43,661 + GFF1195 0.54 -0.5 43,662 - GFF1195 0.55 -0.6 43,662 - GFF1195 0.55 -0.8 43,787 + GFF1196 0.11 +0.1 43,787 + GFF1196 0.11 -0.4 43,787 + GFF1196 0.11 -2.1 43,788 - GFF1196 0.11 +0.8 43,788 - GFF1196 0.11 +0.6 43,788 - GFF1196 0.11 +0.4 43,817 - GFF1196 0.33 +1.0 43,817 - GFF1196 0.33 +0.0 43,817 - GFF1196 0.33 -0.7 43,842 - GFF1196 0.52 -0.6 43,853 - GFF1196 0.61 +2.0 43,887 + GFF1196 0.86 -0.6 44,135 - +0.0 44,173 - -0.2 44,175 - -0.6 44,186 - -1.0 44,281 + GFF1198 0.12 +0.9 44,292 - GFF1198 0.13 -1.5 44,308 + GFF1198 0.16 +0.4 44,384 + GFF1198 0.26 -0.1 44,385 - GFF1198 0.26 -0.8 44,392 + GFF1198 0.27 -0.1 44,393 - GFF1198 0.27 +0.2 44,393 - GFF1198 0.27 +0.0 44,393 - GFF1198 0.27 +0.0 44,443 + GFF1198 0.33 +0.1 44,443 + GFF1198 0.33 -0.1 44,467 - GFF1198 0.36 +0.4 44,496 + GFF1198 0.40 -0.1 44,523 - GFF1198 0.44 +0.7 44,630 - GFF1198 0.58 +0.0 44,630 - GFF1198 0.58 -0.8
Or see this region's nucleotide sequence