Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF621 and GFF622 are separated by 289 nucleotides GFF622 and GFF623 are separated by 95 nucleotides GFF623 and GFF624 are separated by 120 nucleotides GFF624 and GFF625 overlap by 1 nucleotides
GFF621 - IgaA: a membrane protein that prevents overactivation of the Rcs regulatory system, at 360,763 to 362,895
GFF621
GFF622 - ADP compounds hydrolase NudE (EC 3.6.1.-), at 363,185 to 363,781
GFF622
GFF623 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 363,877 to 366,429
GFF623
GFF624 - Type IV pilus biogenesis protein PilM, at 366,550 to 367,329
GFF624
GFF625 - Type IV pilus biogenesis protein PilN, at 367,329 to 367,868
GFF625
Position (kb) 363
364
365
366
367 Strain fitness (log2 ratio) -2
-1
0
1 at 362.910 kb on - strand at 362.913 kb on - strand at 363.032 kb on + strand at 363.033 kb on - strand at 363.039 kb on - strand at 363.174 kb on + strand at 363.232 kb on - strand at 363.340 kb on + strand, within GFF622 at 363.341 kb on - strand, within GFF622 at 363.341 kb on - strand, within GFF622 at 363.403 kb on + strand, within GFF622 at 363.660 kb on + strand, within GFF622 at 363.904 kb on - strand at 364.092 kb on - strand at 364.238 kb on + strand, within GFF623 at 364.359 kb on + strand, within GFF623 at 364.568 kb on + strand, within GFF623 at 364.569 kb on - strand, within GFF623 at 364.661 kb on + strand, within GFF623 at 364.662 kb on - strand, within GFF623 at 364.768 kb on - strand, within GFF623 at 364.879 kb on + strand, within GFF623 at 364.977 kb on + strand, within GFF623 at 365.015 kb on + strand, within GFF623 at 365.235 kb on + strand, within GFF623 at 365.304 kb on + strand, within GFF623 at 365.305 kb on - strand, within GFF623 at 365.308 kb on + strand, within GFF623 at 365.393 kb on + strand, within GFF623 at 365.394 kb on - strand, within GFF623 at 365.501 kb on + strand, within GFF623 at 365.514 kb on + strand, within GFF623 at 365.514 kb on + strand, within GFF623 at 365.612 kb on + strand, within GFF623 at 365.612 kb on + strand, within GFF623 at 365.613 kb on - strand, within GFF623 at 365.613 kb on - strand, within GFF623 at 365.613 kb on - strand, within GFF623 at 365.613 kb on - strand, within GFF623 at 365.642 kb on + strand, within GFF623 at 365.688 kb on + strand, within GFF623 at 365.689 kb on - strand, within GFF623 at 365.728 kb on - strand, within GFF623 at 365.728 kb on - strand, within GFF623 at 365.729 kb on + strand, within GFF623 at 365.741 kb on - strand, within GFF623 at 365.759 kb on - strand, within GFF623 at 365.803 kb on - strand, within GFF623 at 365.808 kb on + strand, within GFF623 at 365.856 kb on + strand, within GFF623 at 365.857 kb on - strand, within GFF623 at 365.857 kb on - strand, within GFF623 at 365.928 kb on - strand, within GFF623 at 365.934 kb on - strand, within GFF623 at 366.073 kb on - strand, within GFF623 at 366.073 kb on - strand, within GFF623 at 366.129 kb on + strand, within GFF623 at 366.159 kb on - strand, within GFF623 at 366.248 kb on + strand at 366.259 kb on - strand at 366.279 kb on - strand at 366.331 kb on + strand at 366.346 kb on - strand at 366.363 kb on - strand at 366.410 kb on - strand at 366.414 kb on - strand at 366.484 kb on + strand at 366.484 kb on + strand at 366.486 kb on + strand at 366.572 kb on + strand at 366.583 kb on - strand at 366.583 kb on - strand at 366.723 kb on - strand, within GFF624 at 366.723 kb on - strand, within GFF624 at 366.752 kb on + strand, within GFF624 at 366.766 kb on + strand, within GFF624 at 366.927 kb on - strand, within GFF624 at 367.041 kb on - strand, within GFF624 at 367.059 kb on + strand, within GFF624 at 367.062 kb on - strand, within GFF624 at 367.204 kb on - strand, within GFF624 at 367.322 kb on + strand at 367.322 kb on + strand at 367.323 kb on - strand at 367.371 kb on + strand at 367.372 kb on - strand at 367.376 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 362,910 - +1.2 362,913 - -0.2 363,032 + -0.7 363,033 - +0.0 363,039 - +0.6 363,174 + -2.7 363,232 - +0.5 363,340 + GFF622 0.26 -0.4 363,341 - GFF622 0.26 -0.2 363,341 - GFF622 0.26 -1.1 363,403 + GFF622 0.37 +0.1 363,660 + GFF622 0.80 -0.3 363,904 - +0.3 364,092 - -0.3 364,238 + GFF623 0.14 -1.1 364,359 + GFF623 0.19 -0.6 364,568 + GFF623 0.27 -1.1 364,569 - GFF623 0.27 +0.3 364,661 + GFF623 0.31 -0.0 364,662 - GFF623 0.31 -1.1 364,768 - GFF623 0.35 +0.0 364,879 + GFF623 0.39 -1.6 364,977 + GFF623 0.43 +0.2 365,015 + GFF623 0.45 -0.1 365,235 + GFF623 0.53 -0.7 365,304 + GFF623 0.56 +0.3 365,305 - GFF623 0.56 -0.8 365,308 + GFF623 0.56 -1.0 365,393 + GFF623 0.59 +0.4 365,394 - GFF623 0.59 -0.6 365,501 + GFF623 0.64 -0.5 365,514 + GFF623 0.64 +0.0 365,514 + GFF623 0.64 +0.6 365,612 + GFF623 0.68 +0.4 365,612 + GFF623 0.68 +0.1 365,613 - GFF623 0.68 -0.9 365,613 - GFF623 0.68 -0.1 365,613 - GFF623 0.68 -0.2 365,613 - GFF623 0.68 -0.9 365,642 + GFF623 0.69 +0.1 365,688 + GFF623 0.71 -0.1 365,689 - GFF623 0.71 -0.4 365,728 - GFF623 0.73 -1.2 365,728 - GFF623 0.73 -0.2 365,729 + GFF623 0.73 -0.8 365,741 - GFF623 0.73 -1.5 365,759 - GFF623 0.74 -0.4 365,803 - GFF623 0.75 -0.6 365,808 + GFF623 0.76 -0.7 365,856 + GFF623 0.78 -0.5 365,857 - GFF623 0.78 -0.9 365,857 - GFF623 0.78 -1.1 365,928 - GFF623 0.80 -0.4 365,934 - GFF623 0.81 +1.2 366,073 - GFF623 0.86 -1.8 366,073 - GFF623 0.86 -1.2 366,129 + GFF623 0.88 -0.3 366,159 - GFF623 0.89 -0.7 366,248 + -2.1 366,259 - -0.6 366,279 - -0.7 366,331 + -0.1 366,346 - -0.3 366,363 - -1.2 366,410 - -1.0 366,414 - -0.7 366,484 + +0.2 366,484 + -0.4 366,486 + -0.5 366,572 + +0.9 366,583 - -0.1 366,583 - +0.1 366,723 - GFF624 0.22 +0.5 366,723 - GFF624 0.22 +0.8 366,752 + GFF624 0.26 +0.3 366,766 + GFF624 0.28 +0.1 366,927 - GFF624 0.48 -0.4 367,041 - GFF624 0.63 +0.3 367,059 + GFF624 0.65 -1.6 367,062 - GFF624 0.66 +0.2 367,204 - GFF624 0.84 -0.3 367,322 + -0.9 367,322 + -0.1 367,323 - +0.5 367,371 + +0.0 367,372 - -0.0 367,376 - -0.3
Or see this region's nucleotide sequence