Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF615 and GFF616 are separated by 53 nucleotides GFF616 and GFF617 are separated by 191 nucleotides
GFF615 - Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49), at 354,897 to 356,516
GFF615
GFF616 - hypothetical protein, at 356,570 to 356,704
GFF616
GFF617 - Putative transport protein, at 356,896 to 358,605
GFF617
Position (kb) 356
357 Strain fitness (log2 ratio) -1
0
1 at 355.839 kb on - strand, within GFF615 at 355.858 kb on + strand, within GFF615 at 355.925 kb on + strand, within GFF615 at 356.018 kb on + strand, within GFF615 at 356.240 kb on + strand, within GFF615 at 356.279 kb on + strand, within GFF615 at 356.306 kb on + strand, within GFF615 at 356.306 kb on + strand, within GFF615 at 356.306 kb on + strand, within GFF615 at 356.307 kb on - strand, within GFF615 at 356.308 kb on + strand, within GFF615 at 356.308 kb on + strand, within GFF615 at 356.308 kb on + strand, within GFF615 at 356.308 kb on + strand, within GFF615 at 356.309 kb on - strand, within GFF615 at 356.309 kb on - strand, within GFF615 at 356.309 kb on - strand, within GFF615 at 356.309 kb on - strand, within GFF615 at 356.312 kb on + strand, within GFF615 at 356.370 kb on - strand at 356.373 kb on + strand at 356.373 kb on + strand at 356.390 kb on - strand at 356.408 kb on + strand at 356.423 kb on - strand at 356.434 kb on + strand at 356.476 kb on + strand at 356.477 kb on - strand at 356.538 kb on + strand at 356.539 kb on - strand at 356.539 kb on - strand at 356.539 kb on - strand at 356.540 kb on + strand at 356.593 kb on + strand, within GFF616 at 356.600 kb on - strand, within GFF616 at 356.736 kb on + strand at 356.737 kb on - strand at 356.761 kb on + strand at 356.919 kb on + strand at 356.920 kb on - strand at 356.922 kb on + strand at 356.922 kb on + strand at 356.971 kb on + strand at 356.972 kb on - strand at 356.972 kb on - strand at 357.003 kb on + strand at 357.020 kb on - strand at 357.020 kb on - strand at 357.020 kb on - strand at 357.024 kb on + strand at 357.057 kb on - strand at 357.057 kb on - strand at 357.064 kb on - strand at 357.078 kb on + strand, within GFF617 at 357.202 kb on + strand, within GFF617 at 357.253 kb on + strand, within GFF617 at 357.320 kb on + strand, within GFF617 at 357.320 kb on + strand, within GFF617 at 357.448 kb on - strand, within GFF617 at 357.452 kb on - strand, within GFF617 at 357.591 kb on + strand, within GFF617 at 357.603 kb on - strand, within GFF617
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 355,839 - GFF615 0.58 +0.2 355,858 + GFF615 0.59 +0.4 355,925 + GFF615 0.63 +0.1 356,018 + GFF615 0.69 -0.2 356,240 + GFF615 0.83 +0.0 356,279 + GFF615 0.85 -1.5 356,306 + GFF615 0.87 +0.0 356,306 + GFF615 0.87 +0.4 356,306 + GFF615 0.87 +0.1 356,307 - GFF615 0.87 -0.3 356,308 + GFF615 0.87 +0.1 356,308 + GFF615 0.87 +0.2 356,308 + GFF615 0.87 -0.8 356,308 + GFF615 0.87 +0.5 356,309 - GFF615 0.87 -0.1 356,309 - GFF615 0.87 -0.1 356,309 - GFF615 0.87 +0.2 356,309 - GFF615 0.87 -0.1 356,312 + GFF615 0.87 +0.2 356,370 - -0.3 356,373 + +0.2 356,373 + +0.3 356,390 - -0.0 356,408 + +0.0 356,423 - -0.4 356,434 + +0.0 356,476 + +0.2 356,477 - +0.5 356,538 + -0.2 356,539 - +0.1 356,539 - +0.3 356,539 - -0.0 356,540 + +0.2 356,593 + GFF616 0.17 -1.6 356,600 - GFF616 0.22 -0.3 356,736 + +0.5 356,737 - -1.2 356,761 + -1.0 356,919 + -0.1 356,920 - -1.3 356,922 + +0.6 356,922 + -1.7 356,971 + -0.8 356,972 - -0.7 356,972 - -0.2 357,003 + -0.1 357,020 - -0.1 357,020 - -0.2 357,020 - +0.2 357,024 + -0.0 357,057 - +0.6 357,057 - +0.3 357,064 - -0.4 357,078 + GFF617 0.11 -0.3 357,202 + GFF617 0.18 +0.5 357,253 + GFF617 0.21 +0.3 357,320 + GFF617 0.25 +0.2 357,320 + GFF617 0.25 +0.1 357,448 - GFF617 0.32 +0.3 357,452 - GFF617 0.33 +0.9 357,591 + GFF617 0.41 +0.2 357,603 - GFF617 0.41 -0.4
Or see this region's nucleotide sequence