Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF496 and GFF497 are separated by 53 nucleotides GFF497 and GFF498 are separated by 53 nucleotides GFF498 and GFF499 are separated by 58 nucleotides
GFF496 - Protein YhjJ, putative peptidase, at 223,070 to 224,515
GFF496
GFF497 - 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45), at 224,569 to 225,498
GFF497
GFF498 - hypothetical protein, at 225,552 to 225,668
GFF498
GFF499 - GGDEF/EAL domain protein YhjH, at 225,727 to 226,494
GFF499
Position (kb) 224
225
226 Strain fitness (log2 ratio) -2
-1
0
1 at 223.822 kb on + strand, within GFF496 at 223.823 kb on - strand, within GFF496 at 223.858 kb on + strand, within GFF496 at 223.859 kb on - strand, within GFF496 at 224.056 kb on + strand, within GFF496 at 224.056 kb on + strand, within GFF496 at 224.057 kb on - strand, within GFF496 at 224.728 kb on + strand, within GFF497 at 224.818 kb on + strand, within GFF497 at 224.949 kb on - strand, within GFF497 at 224.998 kb on - strand, within GFF497 at 224.998 kb on - strand, within GFF497 at 224.998 kb on - strand, within GFF497 at 225.090 kb on + strand, within GFF497 at 225.110 kb on + strand, within GFF497 at 225.110 kb on + strand, within GFF497 at 225.197 kb on + strand, within GFF497 at 225.234 kb on - strand, within GFF497 at 225.234 kb on - strand, within GFF497 at 225.236 kb on + strand, within GFF497 at 225.236 kb on + strand, within GFF497 at 225.237 kb on - strand, within GFF497 at 225.280 kb on - strand, within GFF497 at 225.350 kb on + strand, within GFF497 at 225.351 kb on - strand, within GFF497 at 225.390 kb on + strand, within GFF497 at 225.390 kb on + strand, within GFF497 at 225.392 kb on + strand, within GFF497 at 225.392 kb on + strand, within GFF497 at 225.403 kb on - strand, within GFF497 at 225.403 kb on - strand, within GFF497 at 225.403 kb on - strand, within GFF497 at 225.498 kb on + strand at 225.499 kb on - strand at 225.732 kb on - strand at 225.732 kb on - strand at 225.752 kb on + strand at 225.771 kb on + strand at 225.820 kb on + strand, within GFF499 at 225.826 kb on + strand, within GFF499 at 225.839 kb on - strand, within GFF499 at 225.839 kb on - strand, within GFF499 at 225.912 kb on + strand, within GFF499 at 226.025 kb on + strand, within GFF499 at 226.025 kb on + strand, within GFF499 at 226.107 kb on - strand, within GFF499 at 226.137 kb on + strand, within GFF499 at 226.137 kb on + strand, within GFF499 at 226.261 kb on - strand, within GFF499
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 223,822 + GFF496 0.52 +0.5 223,823 - GFF496 0.52 +0.9 223,858 + GFF496 0.54 +0.2 223,859 - GFF496 0.55 +1.3 224,056 + GFF496 0.68 +0.6 224,056 + GFF496 0.68 +0.6 224,057 - GFF496 0.68 +0.5 224,728 + GFF497 0.17 +0.1 224,818 + GFF497 0.27 +0.2 224,949 - GFF497 0.41 -0.0 224,998 - GFF497 0.46 -0.0 224,998 - GFF497 0.46 +0.2 224,998 - GFF497 0.46 +0.1 225,090 + GFF497 0.56 +0.5 225,110 + GFF497 0.58 -0.8 225,110 + GFF497 0.58 +0.3 225,197 + GFF497 0.68 +0.4 225,234 - GFF497 0.72 -0.0 225,234 - GFF497 0.72 -0.1 225,236 + GFF497 0.72 -0.9 225,236 + GFF497 0.72 -0.1 225,237 - GFF497 0.72 +0.2 225,280 - GFF497 0.76 -0.7 225,350 + GFF497 0.84 -0.1 225,351 - GFF497 0.84 +0.1 225,390 + GFF497 0.88 +0.0 225,390 + GFF497 0.88 +0.5 225,392 + GFF497 0.88 -0.3 225,392 + GFF497 0.88 -0.3 225,403 - GFF497 0.90 -0.3 225,403 - GFF497 0.90 -0.2 225,403 - GFF497 0.90 -0.4 225,498 + -0.1 225,499 - -0.2 225,732 - -1.0 225,732 - -0.7 225,752 + -0.4 225,771 + +1.4 225,820 + GFF499 0.12 -0.8 225,826 + GFF499 0.13 -0.1 225,839 - GFF499 0.15 +0.3 225,839 - GFF499 0.15 -0.8 225,912 + GFF499 0.24 -1.0 226,025 + GFF499 0.39 +0.6 226,025 + GFF499 0.39 +0.2 226,107 - GFF499 0.49 +0.0 226,137 + GFF499 0.53 -2.1 226,137 + GFF499 0.53 -0.2 226,261 - GFF499 0.70 -0.6
Or see this region's nucleotide sequence