Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4914 and GFF4915 overlap by 4 nucleotides GFF4915 and GFF4916 overlap by 4 nucleotides GFF4916 and GFF4917 are separated by 400 nucleotides
GFF4914 - Putative nucleotide di-P-sugar epimerase or dehydratase, at 17,549 to 18,562
GFF4914
GFF4915 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 18,559 to 19,389
GFF4915
GFF4916 - FIG00639119: hypothetical protein, at 19,386 to 19,709
GFF4916
GFF4917 - hypothetical protein, at 20,110 to 20,247
GFF4917
Position (kb) 18
19
20 Strain fitness (log2 ratio) -1
0
1 at 17.777 kb on - strand, within GFF4914 at 17.882 kb on - strand, within GFF4914 at 18.045 kb on + strand, within GFF4914 at 18.082 kb on - strand, within GFF4914 at 18.082 kb on - strand, within GFF4914 at 18.104 kb on - strand, within GFF4914 at 18.117 kb on - strand, within GFF4914 at 18.167 kb on + strand, within GFF4914 at 18.167 kb on + strand, within GFF4914 at 18.243 kb on - strand, within GFF4914 at 18.243 kb on - strand, within GFF4914 at 18.243 kb on - strand, within GFF4914 at 18.274 kb on - strand, within GFF4914 at 18.286 kb on - strand, within GFF4914 at 18.353 kb on - strand, within GFF4914 at 18.445 kb on + strand, within GFF4914 at 18.572 kb on + strand at 18.573 kb on - strand at 18.710 kb on + strand, within GFF4915 at 18.711 kb on - strand, within GFF4915 at 18.921 kb on + strand, within GFF4915 at 18.921 kb on + strand, within GFF4915 at 18.934 kb on - strand, within GFF4915 at 18.937 kb on - strand, within GFF4915 at 19.051 kb on - strand, within GFF4915 at 19.149 kb on - strand, within GFF4915 at 19.149 kb on - strand, within GFF4915 at 19.175 kb on + strand, within GFF4915 at 19.434 kb on - strand, within GFF4916 at 19.434 kb on - strand, within GFF4916 at 19.460 kb on - strand, within GFF4916 at 19.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 17,777 - GFF4914 0.22 -0.7 17,882 - GFF4914 0.33 +0.4 18,045 + GFF4914 0.49 -0.5 18,082 - GFF4914 0.53 +0.3 18,082 - GFF4914 0.53 +0.2 18,104 - GFF4914 0.55 -0.0 18,117 - GFF4914 0.56 +0.2 18,167 + GFF4914 0.61 +0.0 18,167 + GFF4914 0.61 -0.7 18,243 - GFF4914 0.68 +1.0 18,243 - GFF4914 0.68 -0.6 18,243 - GFF4914 0.68 -0.1 18,274 - GFF4914 0.71 -0.3 18,286 - GFF4914 0.73 -0.2 18,353 - GFF4914 0.79 -0.3 18,445 + GFF4914 0.88 -1.7 18,572 + -1.7 18,573 - +0.4 18,710 + GFF4915 0.18 -0.4 18,711 - GFF4915 0.18 -0.7 18,921 + GFF4915 0.44 -0.7 18,921 + GFF4915 0.44 -0.5 18,934 - GFF4915 0.45 -0.5 18,937 - GFF4915 0.45 -1.6 19,051 - GFF4915 0.59 -0.1 19,149 - GFF4915 0.71 -0.9 19,149 - GFF4915 0.71 -0.3 19,175 + GFF4915 0.74 +0.2 19,434 - GFF4916 0.15 -1.1 19,434 - GFF4916 0.15 +0.6 19,460 - GFF4916 0.23 +0.2 19,731 - +0.3
Or see this region's nucleotide sequence