Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF474 and GFF475 are separated by 582 nucleotides GFF475 and GFF476 are separated by 156 nucleotides
GFF474 - Xanthine permease, at 195,168 to 196,493
GFF474
GFF475 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2), at 197,076 to 198,656
GFF475
GFF476 - Dipeptide transport system permease protein DppB (TC 3.A.1.5.2), at 198,813 to 199,832
GFF476
Position (kb)
197
198
199 Strain fitness (log2 ratio)
-1
0
1 at 196.151 kb on - strand, within GFF474 at 196.265 kb on + strand, within GFF474 at 196.266 kb on - strand, within GFF474 at 196.290 kb on - strand, within GFF474 at 196.343 kb on + strand, within GFF474 at 196.344 kb on - strand, within GFF474 at 196.344 kb on - strand, within GFF474 at 196.451 kb on + strand at 196.575 kb on + strand at 196.578 kb on - strand at 196.640 kb on - strand at 196.831 kb on + strand at 196.867 kb on + strand at 197.026 kb on + strand at 197.027 kb on - strand at 197.054 kb on + strand at 197.145 kb on + strand at 197.205 kb on + strand at 197.205 kb on + strand at 197.205 kb on + strand at 197.206 kb on - strand at 197.225 kb on + strand at 197.226 kb on - strand at 197.230 kb on - strand at 197.297 kb on + strand, within GFF475 at 197.312 kb on + strand, within GFF475 at 197.333 kb on - strand, within GFF475 at 197.336 kb on + strand, within GFF475 at 197.336 kb on + strand, within GFF475 at 197.448 kb on - strand, within GFF475 at 197.448 kb on - strand, within GFF475 at 197.466 kb on + strand, within GFF475 at 197.472 kb on + strand, within GFF475 at 197.600 kb on + strand, within GFF475 at 197.600 kb on + strand, within GFF475 at 197.616 kb on + strand, within GFF475 at 197.724 kb on + strand, within GFF475 at 197.928 kb on + strand, within GFF475 at 197.937 kb on + strand, within GFF475 at 197.937 kb on + strand, within GFF475 at 197.938 kb on - strand, within GFF475 at 197.938 kb on - strand, within GFF475 at 197.940 kb on - strand, within GFF475 at 197.940 kb on - strand, within GFF475 at 197.991 kb on + strand, within GFF475 at 198.081 kb on + strand, within GFF475 at 198.092 kb on + strand, within GFF475 at 198.202 kb on - strand, within GFF475 at 198.221 kb on + strand, within GFF475 at 198.282 kb on + strand, within GFF475 at 198.282 kb on + strand, within GFF475 at 198.282 kb on + strand, within GFF475 at 198.438 kb on + strand, within GFF475 at 198.438 kb on + strand, within GFF475 at 198.439 kb on - strand, within GFF475 at 198.440 kb on + strand, within GFF475 at 198.441 kb on - strand, within GFF475 at 198.441 kb on - strand, within GFF475 at 198.508 kb on - strand at 198.750 kb on - strand at 198.750 kb on - strand at 198.980 kb on - strand, within GFF476 at 199.009 kb on - strand, within GFF476 at 199.020 kb on - strand, within GFF476 at 199.020 kb on - strand, within GFF476 at 199.045 kb on + strand, within GFF476 at 199.100 kb on + strand, within GFF476 at 199.239 kb on - strand, within GFF476 at 199.239 kb on - strand, within GFF476 at 199.239 kb on - strand, within GFF476 at 199.239 kb on - strand, within GFF476 at 199.239 kb on - strand, within GFF476 at 199.239 kb on - strand, within GFF476 at 199.407 kb on - strand, within GFF476 at 199.424 kb on + strand, within GFF476 at 199.424 kb on + strand, within GFF476 at 199.425 kb on - strand, within GFF476 at 199.518 kb on + strand, within GFF476 at 199.519 kb on - strand, within GFF476
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 196,151 - GFF474 0.74 -0.4 196,265 + GFF474 0.83 -1.5 196,266 - GFF474 0.83 -0.1 196,290 - GFF474 0.85 +0.4 196,343 + GFF474 0.89 +0.0 196,344 - GFF474 0.89 -1.3 196,344 - GFF474 0.89 -0.3 196,451 + -0.1 196,575 + +0.1 196,578 - -0.0 196,640 - +1.4 196,831 + -0.0 196,867 + +0.5 197,026 + -0.3 197,027 - +0.1 197,054 + -0.4 197,145 + -0.4 197,205 + -0.3 197,205 + -0.0 197,205 + +0.4 197,206 - +0.2 197,225 + +0.3 197,226 - +0.5 197,230 - +0.2 197,297 + GFF475 0.14 -0.6 197,312 + GFF475 0.15 -0.1 197,333 - GFF475 0.16 -1.0 197,336 + GFF475 0.16 -0.4 197,336 + GFF475 0.16 +0.2 197,448 - GFF475 0.24 +1.1 197,448 - GFF475 0.24 +0.3 197,466 + GFF475 0.25 +0.1 197,472 + GFF475 0.25 +0.7 197,600 + GFF475 0.33 -0.3 197,600 + GFF475 0.33 +0.1 197,616 + GFF475 0.34 +0.1 197,724 + GFF475 0.41 -0.1 197,928 + GFF475 0.54 +0.7 197,937 + GFF475 0.54 -0.4 197,937 + GFF475 0.54 -0.1 197,938 - GFF475 0.55 +0.3 197,938 - GFF475 0.55 +0.4 197,940 - GFF475 0.55 +0.2 197,940 - GFF475 0.55 -0.2 197,991 + GFF475 0.58 +0.2 198,081 + GFF475 0.64 +0.1 198,092 + GFF475 0.64 +1.5 198,202 - GFF475 0.71 -0.5 198,221 + GFF475 0.72 +0.2 198,282 + GFF475 0.76 +0.2 198,282 + GFF475 0.76 +0.1 198,282 + GFF475 0.76 +0.4 198,438 + GFF475 0.86 -0.0 198,438 + GFF475 0.86 +0.2 198,439 - GFF475 0.86 +0.3 198,440 + GFF475 0.86 -0.3 198,441 - GFF475 0.86 +0.3 198,441 - GFF475 0.86 +0.3 198,508 - +0.3 198,750 - -0.6 198,750 - -0.2 198,980 - GFF476 0.16 +0.2 199,009 - GFF476 0.19 -0.0 199,020 - GFF476 0.20 -0.8 199,020 - GFF476 0.20 -0.2 199,045 + GFF476 0.23 -0.3 199,100 + GFF476 0.28 +0.7 199,239 - GFF476 0.42 -0.1 199,239 - GFF476 0.42 +0.9 199,239 - GFF476 0.42 +0.1 199,239 - GFF476 0.42 -1.3 199,239 - GFF476 0.42 -0.2 199,239 - GFF476 0.42 +0.1 199,407 - GFF476 0.58 +0.0 199,424 + GFF476 0.60 -0.0 199,424 + GFF476 0.60 +0.1 199,425 - GFF476 0.60 +0.5 199,518 + GFF476 0.69 +0.4 199,519 - GFF476 0.69 -0.4
Or see this region's nucleotide sequence