Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4607 and GFF4608 are separated by 68 nucleotides GFF4608 and GFF4609 are separated by 8 nucleotides GFF4609 and GFF4610 are separated by 20 nucleotides
GFF4607 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 397,557 to 400,169
GFF4607
GFF4608 - Potassium-transporting ATPase C chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 400,238 to 400,822
GFF4608
GFF4609 - Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 400,831 to 402,879
GFF4609
GFF4610 - Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 402,900 to 404,579
GFF4610
Position (kb)
400
401
402
403 Strain fitness (log2 ratio)
-1
0
1
2 at 400.337 kb on - strand, within GFF4608 at 400.337 kb on - strand, within GFF4608 at 400.338 kb on + strand, within GFF4608 at 400.339 kb on - strand, within GFF4608 at 400.432 kb on + strand, within GFF4608 at 400.433 kb on - strand, within GFF4608 at 400.530 kb on - strand, within GFF4608 at 400.592 kb on - strand, within GFF4608 at 400.594 kb on - strand, within GFF4608 at 400.677 kb on - strand, within GFF4608 at 400.690 kb on + strand, within GFF4608 at 400.788 kb on - strand at 400.788 kb on - strand at 400.812 kb on - strand at 400.825 kb on + strand at 400.826 kb on - strand at 400.882 kb on - strand at 401.238 kb on + strand, within GFF4609 at 401.946 kb on + strand, within GFF4609 at 401.946 kb on + strand, within GFF4609 at 402.687 kb on + strand at 402.719 kb on - strand at 402.879 kb on + strand at 402.887 kb on + strand at 402.888 kb on - strand at 402.950 kb on + strand at 402.998 kb on + strand at 403.209 kb on + strand, within GFF4610 at 403.209 kb on + strand, within GFF4610 at 403.210 kb on - strand, within GFF4610 at 403.210 kb on - strand, within GFF4610 at 403.210 kb on - strand, within GFF4610 at 403.439 kb on - strand, within GFF4610 at 403.439 kb on - strand, within GFF4610 at 403.540 kb on - strand, within GFF4610
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 400,337 - GFF4608 0.17 +0.3 400,337 - GFF4608 0.17 -0.2 400,338 + GFF4608 0.17 +0.1 400,339 - GFF4608 0.17 +0.3 400,432 + GFF4608 0.33 +0.1 400,433 - GFF4608 0.33 +0.2 400,530 - GFF4608 0.50 -0.4 400,592 - GFF4608 0.61 +0.0 400,594 - GFF4608 0.61 -0.1 400,677 - GFF4608 0.75 +0.2 400,690 + GFF4608 0.77 +1.0 400,788 - -0.1 400,788 - -0.2 400,812 - -0.2 400,825 + +0.0 400,826 - -0.0 400,882 - +0.0 401,238 + GFF4609 0.20 -1.3 401,946 + GFF4609 0.54 -0.4 401,946 + GFF4609 0.54 -1.0 402,687 + -0.2 402,719 - -0.0 402,879 + +0.2 402,887 + -1.0 402,888 - +0.8 402,950 + -0.6 402,998 + +1.1 403,209 + GFF4610 0.18 +0.9 403,209 + GFF4610 0.18 +1.1 403,210 - GFF4610 0.18 +0.1 403,210 - GFF4610 0.18 +2.2 403,210 - GFF4610 0.18 +0.2 403,439 - GFF4610 0.32 -0.8 403,439 - GFF4610 0.32 -0.3 403,540 - GFF4610 0.38 -0.1
Or see this region's nucleotide sequence