Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4323

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4321 and GFF4322 overlap by 14 nucleotidesGFF4322 and GFF4323 are separated by 103 nucleotidesGFF4323 and GFF4324 are separated by 56 nucleotides GFF4321 - Thiamine-monophosphate kinase (EC 2.7.4.16), at 102,237 to 103,214 GFF4321 GFF4322 - Phosphatidylglycerophosphatase A (EC 3.1.3.27), at 103,201 to 103,707 GFF4322 GFF4323 - FIG00553873: hypothetical protein, at 103,811 to 104,785 GFF4323 GFF4324 - 1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7), at 104,842 to 106,704 GFF4324 Position (kb) 103 104 105Strain fitness (log2 ratio) -1 0 1at 103.442 kb on + strand, within GFF4322at 103.482 kb on + strand, within GFF4322at 103.563 kb on + strand, within GFF4322at 103.729 kb on + strandat 103.760 kb on + strandat 103.761 kb on - strandat 103.761 kb on - strandat 103.911 kb on - strand, within GFF4323at 104.053 kb on + strand, within GFF4323at 104.129 kb on + strand, within GFF4323at 104.328 kb on + strand, within GFF4323at 104.726 kb on - strandat 104.778 kb on + strandat 104.779 kb on - strandat 104.822 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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103,442 + GFF4322 0.48 -1.1
103,482 + GFF4322 0.55 -0.2
103,563 + GFF4322 0.71 -1.5
103,729 + +0.6
103,760 + +1.0
103,761 - -0.3
103,761 - +0.4
103,911 - GFF4323 0.10 +0.2
104,053 + GFF4323 0.25 +0.5
104,129 + GFF4323 0.33 +0.7
104,328 + GFF4323 0.53 -0.1
104,726 - +0.5
104,778 + +0.0
104,779 - -0.1
104,822 + -0.8

Or see this region's nucleotide sequence