Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4153 and GFF4154 overlap by 20 nucleotides GFF4154 and GFF4155 overlap by 4 nucleotides
GFF4153 - Type I secretion system, membrane fusion protein LapC, at 33,621 to 34,811
GFF4153
GFF4154 - Type I secretion system ATPase, LssB family LapB, at 34,792 to 36,972
GFF4154
GFF4155 - Type I secretion system, outer membrane component LapE, at 36,969 to 38,378
GFF4155
Position (kb)
34
35
36
37 Strain fitness (log2 ratio)
-2
-1
0
1 at 33.871 kb on + strand, within GFF4153 at 33.927 kb on - strand, within GFF4153 at 34.177 kb on + strand, within GFF4153 at 34.178 kb on - strand, within GFF4153 at 34.431 kb on + strand, within GFF4153 at 34.432 kb on - strand, within GFF4153 at 34.432 kb on - strand, within GFF4153 at 34.982 kb on - strand at 34.982 kb on - strand at 35.007 kb on + strand at 35.008 kb on - strand at 35.041 kb on + strand, within GFF4154 at 35.049 kb on + strand, within GFF4154 at 35.248 kb on + strand, within GFF4154 at 35.303 kb on - strand, within GFF4154 at 35.722 kb on - strand, within GFF4154 at 35.722 kb on - strand, within GFF4154 at 35.722 kb on - strand, within GFF4154 at 35.768 kb on + strand, within GFF4154 at 35.768 kb on + strand, within GFF4154 at 35.769 kb on - strand, within GFF4154 at 35.802 kb on + strand, within GFF4154 at 35.850 kb on + strand, within GFF4154 at 35.851 kb on - strand, within GFF4154 at 35.859 kb on - strand, within GFF4154 at 35.859 kb on - strand, within GFF4154 at 35.859 kb on - strand, within GFF4154 at 35.859 kb on - strand, within GFF4154 at 35.862 kb on + strand, within GFF4154 at 35.862 kb on + strand, within GFF4154 at 35.904 kb on + strand, within GFF4154 at 35.908 kb on - strand, within GFF4154 at 36.087 kb on + strand, within GFF4154 at 36.088 kb on - strand, within GFF4154 at 36.138 kb on + strand, within GFF4154 at 36.139 kb on - strand, within GFF4154 at 36.205 kb on - strand, within GFF4154 at 36.364 kb on - strand, within GFF4154 at 36.369 kb on - strand, within GFF4154 at 36.466 kb on + strand, within GFF4154 at 36.470 kb on + strand, within GFF4154 at 36.471 kb on - strand, within GFF4154 at 36.471 kb on - strand, within GFF4154 at 36.528 kb on + strand, within GFF4154 at 36.529 kb on - strand, within GFF4154 at 36.585 kb on - strand, within GFF4154 at 36.676 kb on - strand, within GFF4154 at 36.742 kb on - strand, within GFF4154 at 36.832 kb on - strand at 36.896 kb on + strand at 36.950 kb on - strand at 37.040 kb on + strand at 37.042 kb on + strand at 37.042 kb on + strand at 37.108 kb on + strand at 37.177 kb on + strand, within GFF4155 at 37.405 kb on + strand, within GFF4155 at 37.439 kb on - strand, within GFF4155 at 37.442 kb on + strand, within GFF4155 at 37.462 kb on - strand, within GFF4155 at 37.508 kb on - strand, within GFF4155 at 37.799 kb on + strand, within GFF4155 at 37.843 kb on - strand, within GFF4155 at 37.869 kb on + strand, within GFF4155 at 37.870 kb on - strand, within GFF4155 at 37.870 kb on - strand, within GFF4155
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 33,871 + GFF4153 0.21 +0.8 33,927 - GFF4153 0.26 +0.2 34,177 + GFF4153 0.47 +0.9 34,178 - GFF4153 0.47 +0.6 34,431 + GFF4153 0.68 -0.1 34,432 - GFF4153 0.68 +0.3 34,432 - GFF4153 0.68 +0.7 34,982 - +0.0 34,982 - -0.2 35,007 + +0.3 35,008 - -0.4 35,041 + GFF4154 0.11 -0.4 35,049 + GFF4154 0.12 +0.8 35,248 + GFF4154 0.21 +0.1 35,303 - GFF4154 0.23 +1.3 35,722 - GFF4154 0.43 +0.4 35,722 - GFF4154 0.43 +0.4 35,722 - GFF4154 0.43 +0.1 35,768 + GFF4154 0.45 +0.2 35,768 + GFF4154 0.45 +0.2 35,769 - GFF4154 0.45 -0.9 35,802 + GFF4154 0.46 -0.2 35,850 + GFF4154 0.49 -0.9 35,851 - GFF4154 0.49 -1.1 35,859 - GFF4154 0.49 +0.6 35,859 - GFF4154 0.49 -0.2 35,859 - GFF4154 0.49 +1.7 35,859 - GFF4154 0.49 +0.6 35,862 + GFF4154 0.49 +0.0 35,862 + GFF4154 0.49 +0.3 35,904 + GFF4154 0.51 -1.0 35,908 - GFF4154 0.51 +0.1 36,087 + GFF4154 0.59 +0.0 36,088 - GFF4154 0.59 +0.3 36,138 + GFF4154 0.62 -1.1 36,139 - GFF4154 0.62 -0.7 36,205 - GFF4154 0.65 +0.7 36,364 - GFF4154 0.72 -0.2 36,369 - GFF4154 0.72 +0.9 36,466 + GFF4154 0.77 +0.7 36,470 + GFF4154 0.77 +0.3 36,471 - GFF4154 0.77 -0.2 36,471 - GFF4154 0.77 +0.1 36,528 + GFF4154 0.80 -0.3 36,529 - GFF4154 0.80 -0.0 36,585 - GFF4154 0.82 +1.1 36,676 - GFF4154 0.86 +0.0 36,742 - GFF4154 0.89 -0.0 36,832 - -0.0 36,896 + -0.1 36,950 - +0.2 37,040 + -0.2 37,042 + +0.3 37,042 + +0.2 37,108 + -0.5 37,177 + GFF4155 0.15 -0.7 37,405 + GFF4155 0.31 +0.2 37,439 - GFF4155 0.33 +0.3 37,442 + GFF4155 0.34 -0.4 37,462 - GFF4155 0.35 +0.3 37,508 - GFF4155 0.38 +0.0 37,799 + GFF4155 0.59 +0.3 37,843 - GFF4155 0.62 -2.5 37,869 + GFF4155 0.64 +0.6 37,870 - GFF4155 0.64 +0.2 37,870 - GFF4155 0.64 +0.6
Or see this region's nucleotide sequence