Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4114 and GFF4115 are separated by 11 nucleotides GFF4115 and GFF4118 are separated by 115 nucleotides
GFF4114 - Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA, at 71,725 to 72,828
GFF4114
GFF4115 - Gamma-glutamyl phosphate reductase (EC 1.2.1.41), at 72,840 to 74,090
GFF4115
GFF4118 - tRNA-Thr-CGT, at 74,206 to 74,281
GFF4118
Position (kb) 72
73
74
75 Strain fitness (log2 ratio) -2
-1
0
1 at 71.856 kb on + strand, within GFF4114 at 71.862 kb on + strand, within GFF4114 at 72.109 kb on - strand, within GFF4114 at 72.213 kb on + strand, within GFF4114 at 72.299 kb on - strand, within GFF4114 at 72.420 kb on + strand, within GFF4114 at 72.420 kb on + strand, within GFF4114 at 72.421 kb on - strand, within GFF4114 at 72.558 kb on + strand, within GFF4114 at 72.691 kb on - strand, within GFF4114 at 72.714 kb on + strand, within GFF4114 at 72.715 kb on - strand, within GFF4114 at 72.784 kb on + strand at 72.785 kb on - strand at 72.839 kb on + strand at 72.839 kb on + strand at 72.839 kb on + strand at 72.880 kb on - strand at 72.882 kb on - strand at 72.979 kb on + strand, within GFF4115 at 73.194 kb on + strand, within GFF4115 at 73.194 kb on + strand, within GFF4115 at 73.265 kb on - strand, within GFF4115 at 73.285 kb on - strand, within GFF4115 at 73.416 kb on + strand, within GFF4115 at 73.416 kb on + strand, within GFF4115 at 73.416 kb on + strand, within GFF4115 at 73.416 kb on + strand, within GFF4115 at 73.417 kb on - strand, within GFF4115 at 73.457 kb on + strand, within GFF4115 at 73.717 kb on + strand, within GFF4115 at 73.848 kb on - strand, within GFF4115 at 73.886 kb on + strand, within GFF4115 at 74.049 kb on + strand at 74.051 kb on + strand at 74.088 kb on + strand at 74.167 kb on + strand at 74.213 kb on + strand at 74.256 kb on - strand, within GFF4118 at 74.362 kb on + strand at 74.363 kb on - strand at 74.373 kb on - strand at 74.486 kb on - strand at 74.506 kb on - strand at 74.509 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 71,856 + GFF4114 0.12 -1.3 71,862 + GFF4114 0.12 +0.1 72,109 - GFF4114 0.35 +0.1 72,213 + GFF4114 0.44 +0.3 72,299 - GFF4114 0.52 -0.5 72,420 + GFF4114 0.63 +1.6 72,420 + GFF4114 0.63 +1.3 72,421 - GFF4114 0.63 +0.6 72,558 + GFF4114 0.75 +0.3 72,691 - GFF4114 0.88 -0.1 72,714 + GFF4114 0.90 -0.8 72,715 - GFF4114 0.90 -0.2 72,784 + -0.5 72,785 - -0.5 72,839 + +0.0 72,839 + -0.6 72,839 + -0.2 72,880 - -0.8 72,882 - +0.1 72,979 + GFF4115 0.11 -0.4 73,194 + GFF4115 0.28 -0.5 73,194 + GFF4115 0.28 -0.2 73,265 - GFF4115 0.34 +0.2 73,285 - GFF4115 0.36 -0.4 73,416 + GFF4115 0.46 -0.1 73,416 + GFF4115 0.46 +0.2 73,416 + GFF4115 0.46 +0.8 73,416 + GFF4115 0.46 -0.3 73,417 - GFF4115 0.46 -0.6 73,457 + GFF4115 0.49 -1.8 73,717 + GFF4115 0.70 -1.1 73,848 - GFF4115 0.81 -0.2 73,886 + GFF4115 0.84 +0.1 74,049 + -0.5 74,051 + -1.0 74,088 + -0.5 74,167 + -0.2 74,213 + -0.3 74,256 - GFF4118 0.66 -0.8 74,362 + -0.6 74,363 - +0.2 74,373 - +0.4 74,486 - -0.9 74,506 - -1.3 74,509 + -0.7
Or see this region's nucleotide sequence