Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3954 and GFF3955 overlap by 11 nucleotides GFF3955 and GFF3956 overlap by 17 nucleotides GFF3956 and GFF3957 overlap by 20 nucleotides
GFF3954 - Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-), at 235,163 to 235,768
GFF3954
GFF3955 - Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-), at 235,758 to 236,336
GFF3955
GFF3956 - Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133), at 236,320 to 237,093
GFF3956
GFF3957 - Cobalamin biosynthesis protein CbiG, at 237,074 to 238,129
GFF3957
Position (kb) 236
237
238 Strain fitness (log2 ratio) -1
0
1
2
3 at 235.475 kb on + strand, within GFF3954 at 235.475 kb on + strand, within GFF3954 at 235.475 kb on + strand, within GFF3954 at 235.476 kb on - strand, within GFF3954 at 236.266 kb on - strand, within GFF3955 at 236.349 kb on - strand at 236.394 kb on + strand at 236.427 kb on - strand, within GFF3956 at 236.430 kb on + strand, within GFF3956 at 236.434 kb on + strand, within GFF3956 at 236.470 kb on + strand, within GFF3956 at 236.610 kb on - strand, within GFF3956 at 236.643 kb on + strand, within GFF3956 at 236.658 kb on - strand, within GFF3956 at 236.658 kb on - strand, within GFF3956 at 236.712 kb on + strand, within GFF3956 at 236.713 kb on - strand, within GFF3956 at 236.743 kb on - strand, within GFF3956 at 236.763 kb on + strand, within GFF3956 at 236.828 kb on - strand, within GFF3956 at 236.976 kb on - strand, within GFF3956 at 236.976 kb on - strand, within GFF3956 at 237.176 kb on - strand at 237.176 kb on - strand at 237.250 kb on + strand, within GFF3957 at 237.269 kb on - strand, within GFF3957 at 237.505 kb on - strand, within GFF3957 at 237.518 kb on - strand, within GFF3957 at 237.650 kb on - strand, within GFF3957 at 237.911 kb on - strand, within GFF3957
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 235,475 + GFF3954 0.51 +2.5 235,475 + GFF3954 0.51 -1.0 235,475 + GFF3954 0.51 +0.8 235,476 - GFF3954 0.52 +1.4 236,266 - GFF3955 0.88 +0.1 236,349 - +0.6 236,394 + +0.1 236,427 - GFF3956 0.14 +3.0 236,430 + GFF3956 0.14 +0.2 236,434 + GFF3956 0.15 +0.8 236,470 + GFF3956 0.19 +0.3 236,610 - GFF3956 0.37 +1.8 236,643 + GFF3956 0.42 +2.2 236,658 - GFF3956 0.44 +0.4 236,658 - GFF3956 0.44 -0.4 236,712 + GFF3956 0.51 +0.2 236,713 - GFF3956 0.51 +0.2 236,743 - GFF3956 0.55 -0.8 236,763 + GFF3956 0.57 -0.4 236,828 - GFF3956 0.66 -0.4 236,976 - GFF3956 0.85 +0.2 236,976 - GFF3956 0.85 +1.0 237,176 - +0.4 237,176 - +0.0 237,250 + GFF3957 0.17 +1.9 237,269 - GFF3957 0.18 +0.1 237,505 - GFF3957 0.41 +0.8 237,518 - GFF3957 0.42 -1.3 237,650 - GFF3957 0.55 +0.8 237,911 - GFF3957 0.79 +0.3
Or see this region's nucleotide sequence