Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3939 and GFF3940 are separated by 24 nucleotides GFF3940 and GFF3941 are separated by 18 nucleotides GFF3941 and GFF3942 overlap by 11 nucleotides GFF3942 and GFF3943 are separated by 9 nucleotides GFF3943 and GFF3944 are separated by 14 nucleotides
GFF3939 - Propanediol utilization polyhedral body protein PduK, at 221,048 to 221,509
GFF3939
GFF3940 - Propanediol utilization polyhedral body protein PduJ, at 221,534 to 221,809
GFF3940
GFF3941 - Propanediol dehydratase reactivation factor small subunit, at 221,828 to 222,178
GFF3941
GFF3942 - Propanediol dehydratase reactivation factor large subunit, at 222,168 to 224,000
GFF3942
GFF3943 - Propanediol dehydratase small subunit (EC 4.2.1.28), at 224,010 to 224,531
GFF3943
GFF3944 - Propanediol dehydratase medium subunit (EC 4.2.1.28), at 224,546 to 225,220
GFF3944
Position (kb) 222
223
224
225 Strain fitness (log2 ratio) -2
-1
0
1 at 221.724 kb on - strand, within GFF3940 at 221.829 kb on + strand at 221.958 kb on + strand, within GFF3941 at 221.958 kb on + strand, within GFF3941 at 221.959 kb on - strand, within GFF3941 at 222.857 kb on + strand, within GFF3942 at 222.857 kb on + strand, within GFF3942 at 222.858 kb on - strand, within GFF3942 at 223.260 kb on - strand, within GFF3942 at 223.441 kb on + strand, within GFF3942 at 223.526 kb on + strand, within GFF3942 at 223.913 kb on + strand at 223.971 kb on - strand at 223.991 kb on + strand at 223.992 kb on - strand at 223.992 kb on - strand at 223.994 kb on - strand at 224.045 kb on - strand at 224.164 kb on + strand, within GFF3943 at 224.493 kb on + strand at 224.493 kb on + strand at 224.538 kb on - strand at 224.592 kb on + strand at 224.606 kb on - strand at 224.609 kb on - strand at 224.635 kb on - strand, within GFF3944 at 224.728 kb on - strand, within GFF3944 at 224.812 kb on + strand, within GFF3944 at 224.812 kb on + strand, within GFF3944 at 224.813 kb on - strand, within GFF3944
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 221,724 - GFF3940 0.69 +1.0 221,829 + +0.0 221,958 + GFF3941 0.37 -0.5 221,958 + GFF3941 0.37 -0.4 221,959 - GFF3941 0.37 +0.5 222,857 + GFF3942 0.38 -0.3 222,857 + GFF3942 0.38 -0.3 222,858 - GFF3942 0.38 -2.0 223,260 - GFF3942 0.60 +0.2 223,441 + GFF3942 0.69 -1.0 223,526 + GFF3942 0.74 -1.3 223,913 + -1.0 223,971 - +0.8 223,991 + +0.2 223,992 - +0.5 223,992 - -0.3 223,994 - -0.2 224,045 - +0.2 224,164 + GFF3943 0.30 -0.8 224,493 + -0.4 224,493 + +1.4 224,538 - +0.2 224,592 + +0.7 224,606 - -0.6 224,609 - -0.1 224,635 - GFF3944 0.13 -1.1 224,728 - GFF3944 0.27 +0.1 224,812 + GFF3944 0.39 +1.4 224,812 + GFF3944 0.39 -0.2 224,813 - GFF3944 0.40 +0.4
Or see this region's nucleotide sequence