Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3866

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3864 and GFF3865 are separated by 625 nucleotidesGFF3865 and GFF3866 are separated by 19 nucleotidesGFF3866 and GFF3867 are separated by 5 nucleotidesGFF3867 and GFF3868 overlap by 4 nucleotides GFF3864 - Putative capsular polysaccharide transport protein YegH, at 139,947 to 141,527 GFF3864 GFF3865 - hypothetical protein, at 142,153 to 142,266 GFF3865 GFF3866 - Polysaccharide export lipoprotein Wza, at 142,286 to 143,341 GFF3866 GFF3867 - Low molecular weight protein-tyrosine-phosphatase Wzb (EC 3.1.3.48), at 143,347 to 143,796 GFF3867 GFF3868 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 143,793 to 145,952 GFF3868 Position (kb) 142 143 144Strain fitness (log2 ratio) -1 0 1 2 3at 141.438 kb on - strandat 141.481 kb on - strandat 141.528 kb on - strandat 142.000 kb on + strandat 142.390 kb on - strandat 142.448 kb on + strand, within GFF3866at 142.449 kb on - strand, within GFF3866at 142.454 kb on + strand, within GFF3866at 142.535 kb on - strand, within GFF3866at 142.535 kb on - strand, within GFF3866at 142.584 kb on - strand, within GFF3866at 142.664 kb on + strand, within GFF3866at 142.665 kb on - strand, within GFF3866at 142.763 kb on - strand, within GFF3866at 142.763 kb on - strand, within GFF3866at 142.766 kb on - strand, within GFF3866at 142.943 kb on + strand, within GFF3866at 142.944 kb on - strand, within GFF3866at 142.944 kb on - strand, within GFF3866at 143.165 kb on - strand, within GFF3866at 143.217 kb on - strand, within GFF3866at 143.572 kb on + strand, within GFF3867at 143.572 kb on + strand, within GFF3867at 143.649 kb on - strand, within GFF3867at 143.735 kb on + strand, within GFF3867at 143.908 kb on + strandat 143.933 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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141,438 - +0.3
141,481 - -0.5
141,528 - +0.0
142,000 + +0.7
142,390 - +1.7
142,448 + GFF3866 0.15 +0.1
142,449 - GFF3866 0.15 +0.3
142,454 + GFF3866 0.16 +0.3
142,535 - GFF3866 0.24 -0.5
142,535 - GFF3866 0.24 -0.2
142,584 - GFF3866 0.28 +1.2
142,664 + GFF3866 0.36 -0.1
142,665 - GFF3866 0.36 -0.4
142,763 - GFF3866 0.45 +0.0
142,763 - GFF3866 0.45 +0.2
142,766 - GFF3866 0.45 +0.0
142,943 + GFF3866 0.62 +0.5
142,944 - GFF3866 0.62 +0.8
142,944 - GFF3866 0.62 +0.2
143,165 - GFF3866 0.83 +0.4
143,217 - GFF3866 0.88 -1.4
143,572 + GFF3867 0.50 -0.7
143,572 + GFF3867 0.50 +2.8
143,649 - GFF3867 0.67 +0.7
143,735 + GFF3867 0.86 +0.5
143,908 + -0.2
143,933 + +0.2

Or see this region's nucleotide sequence