Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3864 and GFF3865 are separated by 625 nucleotides GFF3865 and GFF3866 are separated by 19 nucleotides GFF3866 and GFF3867 are separated by 5 nucleotides GFF3867 and GFF3868 overlap by 4 nucleotides
GFF3864 - Putative capsular polysaccharide transport protein YegH, at 139,947 to 141,527
GFF3864
GFF3865 - hypothetical protein, at 142,153 to 142,266
GFF3865
GFF3866 - Polysaccharide export lipoprotein Wza, at 142,286 to 143,341
GFF3866
GFF3867 - Low molecular weight protein-tyrosine-phosphatase Wzb (EC 3.1.3.48), at 143,347 to 143,796
GFF3867
GFF3868 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 143,793 to 145,952
GFF3868
Position (kb)
142
143
144 Strain fitness (log2 ratio)
-1
0
1
2
3 at 141.438 kb on - strand at 141.481 kb on - strand at 141.528 kb on - strand at 142.000 kb on + strand at 142.390 kb on - strand at 142.448 kb on + strand, within GFF3866 at 142.449 kb on - strand, within GFF3866 at 142.454 kb on + strand, within GFF3866 at 142.535 kb on - strand, within GFF3866 at 142.535 kb on - strand, within GFF3866 at 142.584 kb on - strand, within GFF3866 at 142.664 kb on + strand, within GFF3866 at 142.665 kb on - strand, within GFF3866 at 142.763 kb on - strand, within GFF3866 at 142.763 kb on - strand, within GFF3866 at 142.766 kb on - strand, within GFF3866 at 142.943 kb on + strand, within GFF3866 at 142.944 kb on - strand, within GFF3866 at 142.944 kb on - strand, within GFF3866 at 143.165 kb on - strand, within GFF3866 at 143.217 kb on - strand, within GFF3866 at 143.572 kb on + strand, within GFF3867 at 143.572 kb on + strand, within GFF3867 at 143.649 kb on - strand, within GFF3867 at 143.735 kb on + strand, within GFF3867 at 143.908 kb on + strand at 143.933 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 141,438 - +0.3 141,481 - -0.5 141,528 - +0.0 142,000 + +0.7 142,390 - +1.7 142,448 + GFF3866 0.15 +0.1 142,449 - GFF3866 0.15 +0.3 142,454 + GFF3866 0.16 +0.3 142,535 - GFF3866 0.24 -0.5 142,535 - GFF3866 0.24 -0.2 142,584 - GFF3866 0.28 +1.2 142,664 + GFF3866 0.36 -0.1 142,665 - GFF3866 0.36 -0.4 142,763 - GFF3866 0.45 +0.0 142,763 - GFF3866 0.45 +0.2 142,766 - GFF3866 0.45 +0.0 142,943 + GFF3866 0.62 +0.5 142,944 - GFF3866 0.62 +0.8 142,944 - GFF3866 0.62 +0.2 143,165 - GFF3866 0.83 +0.4 143,217 - GFF3866 0.88 -1.4 143,572 + GFF3867 0.50 -0.7 143,572 + GFF3867 0.50 +2.8 143,649 - GFF3867 0.67 +0.7 143,735 + GFF3867 0.86 +0.5 143,908 + -0.2 143,933 + +0.2
Or see this region's nucleotide sequence