Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3701

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3700 and GFF3701 are separated by 11 nucleotidesGFF3701 and GFF3702 are separated by 109 nucleotidesGFF3702 and GFF3703 are separated by 0 nucleotidesGFF3703 and GFF3704 overlap by 4 nucleotides GFF3700 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 6,100 to 7,473 GFF3700 GFF3701 - Nitrite transporter NirC, at 7,485 to 8,294 GFF3701 GFF3702 - hypothetical protein, at 8,404 to 8,559 GFF3702 GFF3703 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 8,560 to 8,886 GFF3703 GFF3704 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 8,883 to 11,390 GFF3704 Position (kb) 7 8 9Strain fitness (log2 ratio) -1 0 1at 6.709 kb on - strand, within GFF3700at 7.140 kb on + strand, within GFF3700at 7.141 kb on - strand, within GFF3700at 7.321 kb on + strand, within GFF3700at 7.486 kb on + strandat 7.596 kb on - strand, within GFF3701at 7.644 kb on - strand, within GFF3701at 7.645 kb on + strand, within GFF3701at 7.646 kb on - strand, within GFF3701at 7.749 kb on - strand, within GFF3701at 7.927 kb on + strand, within GFF3701at 7.985 kb on + strand, within GFF3701at 8.127 kb on + strand, within GFF3701at 8.185 kb on + strand, within GFF3701at 8.186 kb on - strand, within GFF3701at 8.186 kb on - strand, within GFF3701at 8.273 kb on + strandat 8.366 kb on + strandat 8.366 kb on + strandat 8.374 kb on + strandat 8.374 kb on + strandat 8.375 kb on - strandat 8.375 kb on - strandat 8.501 kb on + strand, within GFF3702at 8.590 kb on + strandat 8.638 kb on + strand, within GFF3703at 8.786 kb on + strand, within GFF3703at 8.787 kb on - strand, within GFF3703at 8.923 kb on + strandat 9.208 kb on + strand, within GFF3704at 9.209 kb on - strand, within GFF3704at 9.218 kb on + strand, within GFF3704at 9.233 kb on - strand, within GFF3704

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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6,709 - GFF3700 0.44 +0.2
7,140 + GFF3700 0.76 -0.6
7,141 - GFF3700 0.76 -0.8
7,321 + GFF3700 0.89 -1.1
7,486 + -1.0
7,596 - GFF3701 0.14 -0.5
7,644 - GFF3701 0.20 +0.1
7,645 + GFF3701 0.20 +0.2
7,646 - GFF3701 0.20 +0.2
7,749 - GFF3701 0.33 -0.0
7,927 + GFF3701 0.55 +0.1
7,985 + GFF3701 0.62 -0.2
8,127 + GFF3701 0.79 -0.8
8,185 + GFF3701 0.86 +0.7
8,186 - GFF3701 0.87 +0.4
8,186 - GFF3701 0.87 +0.0
8,273 + +0.7
8,366 + +0.0
8,366 + +0.2
8,374 + +0.3
8,374 + +1.3
8,375 - -0.1
8,375 - -0.4
8,501 + GFF3702 0.62 +0.7
8,590 + +0.2
8,638 + GFF3703 0.24 -0.2
8,786 + GFF3703 0.69 -0.8
8,787 - GFF3703 0.69 +0.1
8,923 + +0.2
9,208 + GFF3704 0.13 +0.3
9,209 - GFF3704 0.13 +0.5
9,218 + GFF3704 0.13 -0.1
9,233 - GFF3704 0.14 +0.1

Or see this region's nucleotide sequence