Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2984 and GFF2985 are separated by 85 nucleotides GFF2985 and GFF2986 are separated by 50 nucleotides GFF2986 and GFF2987 are separated by 75 nucleotides
GFF2984 - Uncharacterized lipoprotein YgeR precursor, at 2,709 to 3,461
GFF2984
GFF2985 - hypothetical protein, at 3,547 to 3,681
GFF2985
GFF2986 - Isopentenyl-diphosphate delta-isomerase (EC 5.3.3.2), at 3,732 to 4,277
GFF2986
GFF2987 - Lysyl-tRNA synthetase (class II) (EC 6.1.1.6), at 4,353 to 5,870
GFF2987
Position (kb)
3
4
5 Strain fitness (log2 ratio)
-1
0
1 at 2.791 kb on - strand, within GFF2984 at 2.791 kb on - strand, within GFF2984 at 2.883 kb on - strand, within GFF2984 at 2.897 kb on + strand, within GFF2984 at 2.898 kb on - strand, within GFF2984 at 2.902 kb on + strand, within GFF2984 at 2.918 kb on + strand, within GFF2984 at 3.069 kb on - strand, within GFF2984 at 3.084 kb on + strand, within GFF2984 at 3.242 kb on + strand, within GFF2984 at 3.243 kb on - strand, within GFF2984 at 3.246 kb on - strand, within GFF2984 at 3.247 kb on + strand, within GFF2984 at 3.248 kb on - strand, within GFF2984 at 3.312 kb on - strand, within GFF2984 at 3.341 kb on + strand, within GFF2984 at 3.343 kb on + strand, within GFF2984 at 3.426 kb on - strand at 3.497 kb on + strand at 3.567 kb on + strand, within GFF2985 at 3.584 kb on + strand, within GFF2985 at 3.585 kb on - strand, within GFF2985 at 3.585 kb on - strand, within GFF2985 at 3.588 kb on - strand, within GFF2985 at 3.642 kb on - strand, within GFF2985 at 3.683 kb on + strand at 3.684 kb on - strand at 3.684 kb on - strand at 3.684 kb on - strand at 3.684 kb on - strand at 3.806 kb on + strand, within GFF2986 at 3.813 kb on - strand, within GFF2986 at 3.815 kb on + strand, within GFF2986 at 3.847 kb on + strand, within GFF2986 at 4.146 kb on + strand, within GFF2986 at 4.355 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 2,791 - GFF2984 0.11 -0.3 2,791 - GFF2984 0.11 -0.8 2,883 - GFF2984 0.23 -0.4 2,897 + GFF2984 0.25 +0.2 2,898 - GFF2984 0.25 +1.7 2,902 + GFF2984 0.26 +0.8 2,918 + GFF2984 0.28 +0.5 3,069 - GFF2984 0.48 -0.9 3,084 + GFF2984 0.50 -0.7 3,242 + GFF2984 0.71 -0.3 3,243 - GFF2984 0.71 +0.1 3,246 - GFF2984 0.71 +0.3 3,247 + GFF2984 0.71 -0.2 3,248 - GFF2984 0.72 -0.2 3,312 - GFF2984 0.80 -0.4 3,341 + GFF2984 0.84 -0.3 3,343 + GFF2984 0.84 +0.0 3,426 - -0.1 3,497 + +0.1 3,567 + GFF2985 0.15 -0.1 3,584 + GFF2985 0.27 -0.1 3,585 - GFF2985 0.28 -0.1 3,585 - GFF2985 0.28 -0.4 3,588 - GFF2985 0.30 +0.4 3,642 - GFF2985 0.70 -0.7 3,683 + -0.4 3,684 - -1.2 3,684 - +0.4 3,684 - -1.1 3,684 - -0.0 3,806 + GFF2986 0.14 -0.5 3,813 - GFF2986 0.15 -0.8 3,815 + GFF2986 0.15 -0.3 3,847 + GFF2986 0.21 +0.2 4,146 + GFF2986 0.76 -1.4 4,355 - -0.2
Or see this region's nucleotide sequence