Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2515 and GFF2516 are separated by 731 nucleotides GFF2516 and GFF2517 are separated by 140 nucleotides GFF2517 and GFF2518 are separated by 12 nucleotides
GFF2515 - putative outer membrane protein, at 30,773 to 31,300
GFF2515
GFF2516 - Outer membrane protein C precursor, at 32,032 to 33,150
GFF2516
GFF2517 - [NiFe] hydrogenase nickel incorporation HybF-like protein, at 33,291 to 33,632
GFF2517
GFF2518 - Putative dehydrogenase protein, at 33,645 to 34,532
GFF2518
Position (kb)
32
33
34 Strain fitness (log2 ratio)
-1
0
1 at 31.175 kb on - strand, within GFF2515 at 31.303 kb on + strand at 31.304 kb on - strand at 31.350 kb on + strand at 31.949 kb on + strand at 31.962 kb on + strand at 32.028 kb on + strand at 32.029 kb on - strand at 32.051 kb on + strand at 32.179 kb on - strand, within GFF2516 at 32.181 kb on + strand, within GFF2516 at 32.217 kb on + strand, within GFF2516 at 32.393 kb on - strand, within GFF2516 at 32.430 kb on - strand, within GFF2516 at 32.608 kb on - strand, within GFF2516 at 32.795 kb on - strand, within GFF2516 at 33.010 kb on - strand, within GFF2516 at 33.045 kb on + strand at 33.175 kb on + strand at 33.181 kb on + strand at 33.404 kb on + strand, within GFF2517 at 33.656 kb on + strand at 33.657 kb on - strand at 33.681 kb on + strand at 33.716 kb on + strand at 33.780 kb on - strand, within GFF2518 at 33.858 kb on + strand, within GFF2518 at 33.966 kb on - strand, within GFF2518 at 34.039 kb on - strand, within GFF2518 at 34.077 kb on - strand, within GFF2518 at 34.088 kb on + strand, within GFF2518
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 31,175 - GFF2515 0.76 +1.0 31,303 + +1.4 31,304 - -0.1 31,350 + -0.3 31,949 + -0.0 31,962 + -0.6 32,028 + -0.2 32,029 - -0.1 32,051 + -0.7 32,179 - GFF2516 0.13 -0.8 32,181 + GFF2516 0.13 -1.3 32,217 + GFF2516 0.17 +0.8 32,393 - GFF2516 0.32 -0.8 32,430 - GFF2516 0.36 -1.7 32,608 - GFF2516 0.51 +0.0 32,795 - GFF2516 0.68 -0.5 33,010 - GFF2516 0.87 -0.6 33,045 + -0.1 33,175 + +0.0 33,181 + +0.3 33,404 + GFF2517 0.33 +0.1 33,656 + +1.0 33,657 - -0.4 33,681 + -1.0 33,716 + -1.2 33,780 - GFF2518 0.15 -0.3 33,858 + GFF2518 0.24 -0.2 33,966 - GFF2518 0.36 -0.2 34,039 - GFF2518 0.44 -0.2 34,077 - GFF2518 0.49 -0.3 34,088 + GFF2518 0.50 -0.1
Or see this region's nucleotide sequence