Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2342 and GFF2343 are separated by 368 nucleotides GFF2343 and GFF2344 are separated by 88 nucleotides
GFF2342 - Phosphoenolpyruvate carboxylase (EC 4.1.1.31), at 134,177 to 136,828
GFF2342
GFF2343 - Acetylornithine deacetylase (EC 3.5.1.16), at 137,197 to 138,348
GFF2343
GFF2344 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 138,437 to 139,441
GFF2344
Position (kb) 137
138
139 Strain fitness (log2 ratio) -2
-1
0
1 at 136.267 kb on + strand, within GFF2342 at 136.308 kb on - strand, within GFF2342 at 136.340 kb on - strand, within GFF2342 at 136.340 kb on - strand, within GFF2342 at 136.355 kb on + strand, within GFF2342 at 136.394 kb on - strand, within GFF2342 at 136.451 kb on + strand, within GFF2342 at 136.452 kb on - strand, within GFF2342 at 136.790 kb on - strand at 136.790 kb on - strand at 136.801 kb on + strand at 136.828 kb on + strand at 136.857 kb on - strand at 136.907 kb on - strand at 137.088 kb on + strand at 137.192 kb on - strand at 137.192 kb on - strand at 137.198 kb on + strand at 137.199 kb on - strand at 137.219 kb on - strand at 137.221 kb on + strand at 137.222 kb on - strand at 137.222 kb on - strand at 137.270 kb on + strand at 137.329 kb on + strand, within GFF2343 at 137.330 kb on - strand, within GFF2343 at 137.355 kb on + strand, within GFF2343 at 137.360 kb on + strand, within GFF2343 at 137.455 kb on + strand, within GFF2343 at 137.515 kb on - strand, within GFF2343 at 137.536 kb on + strand, within GFF2343 at 137.797 kb on - strand, within GFF2343 at 137.802 kb on + strand, within GFF2343 at 137.802 kb on + strand, within GFF2343 at 137.870 kb on - strand, within GFF2343 at 137.978 kb on - strand, within GFF2343 at 138.033 kb on - strand, within GFF2343 at 138.039 kb on + strand, within GFF2343 at 138.039 kb on + strand, within GFF2343 at 138.160 kb on - strand, within GFF2343 at 138.199 kb on + strand, within GFF2343 at 138.199 kb on + strand, within GFF2343 at 138.425 kb on + strand at 138.425 kb on + strand at 138.426 kb on - strand at 138.489 kb on + strand at 138.495 kb on - strand at 138.514 kb on + strand at 138.514 kb on + strand at 138.514 kb on + strand at 138.515 kb on - strand at 138.515 kb on - strand at 138.515 kb on - strand at 138.515 kb on - strand at 138.579 kb on + strand, within GFF2344 at 138.634 kb on - strand, within GFF2344 at 138.777 kb on - strand, within GFF2344 at 138.777 kb on - strand, within GFF2344 at 138.834 kb on - strand, within GFF2344 at 138.972 kb on + strand, within GFF2344 at 139.055 kb on + strand, within GFF2344 at 139.165 kb on + strand, within GFF2344 at 139.165 kb on + strand, within GFF2344 at 139.188 kb on + strand, within GFF2344 at 139.227 kb on - strand, within GFF2344 at 139.254 kb on - strand, within GFF2344
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 136,267 + GFF2342 0.79 -0.3 136,308 - GFF2342 0.80 -0.1 136,340 - GFF2342 0.82 +0.7 136,340 - GFF2342 0.82 +0.3 136,355 + GFF2342 0.82 -0.1 136,394 - GFF2342 0.84 -0.3 136,451 + GFF2342 0.86 -0.0 136,452 - GFF2342 0.86 -2.4 136,790 - +0.7 136,790 - -1.1 136,801 + +0.5 136,828 + +0.2 136,857 - -0.8 136,907 - -1.0 137,088 + -0.6 137,192 - +0.8 137,192 - -0.7 137,198 + -0.1 137,199 - +0.2 137,219 - -0.5 137,221 + -0.3 137,222 - -0.4 137,222 - +0.5 137,270 + -0.5 137,329 + GFF2343 0.11 -0.2 137,330 - GFF2343 0.12 +0.1 137,355 + GFF2343 0.14 +0.2 137,360 + GFF2343 0.14 +1.4 137,455 + GFF2343 0.22 -0.2 137,515 - GFF2343 0.28 -0.3 137,536 + GFF2343 0.29 +0.8 137,797 - GFF2343 0.52 +0.9 137,802 + GFF2343 0.53 -0.8 137,802 + GFF2343 0.53 -0.1 137,870 - GFF2343 0.58 -0.2 137,978 - GFF2343 0.68 +0.3 138,033 - GFF2343 0.73 +0.8 138,039 + GFF2343 0.73 -1.0 138,039 + GFF2343 0.73 +0.3 138,160 - GFF2343 0.84 +0.4 138,199 + GFF2343 0.87 +0.7 138,199 + GFF2343 0.87 -0.5 138,425 + -0.3 138,425 + +0.2 138,426 - +0.1 138,489 + -0.2 138,495 - +0.2 138,514 + -0.3 138,514 + +0.1 138,514 + -0.2 138,515 - -0.1 138,515 - +0.0 138,515 - -0.3 138,515 - +0.5 138,579 + GFF2344 0.14 -0.2 138,634 - GFF2344 0.20 +0.2 138,777 - GFF2344 0.34 +0.7 138,777 - GFF2344 0.34 +0.3 138,834 - GFF2344 0.40 +0.2 138,972 + GFF2344 0.53 -0.6 139,055 + GFF2344 0.61 +0.0 139,165 + GFF2344 0.72 +0.2 139,165 + GFF2344 0.72 -0.2 139,188 + GFF2344 0.75 -0.1 139,227 - GFF2344 0.79 +0.5 139,254 - GFF2344 0.81 +0.1
Or see this region's nucleotide sequence