Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2179

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2177 and GFF2178 are separated by 46 nucleotidesGFF2178 and GFF2179 overlap by 1 nucleotidesGFF2179 and GFF2180 are separated by 145 nucleotides GFF2177 - Glycerate kinase (EC 2.7.1.31), at 24,875 to 25,228 GFF2177 GFF2178 - Thiol peroxidase, Bcp-type (EC 1.11.1.15), at 25,275 to 25,745 GFF2178 GFF2179 - Glycine cleavage system transcriptional antiactivator GcvR, at 25,745 to 26,317 GFF2179 GFF2180 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 26,463 to 27,341 GFF2180 Position (kb) 25 26 27Strain fitness (log2 ratio) -1 0 1at 25.155 kb on + strand, within GFF2177at 25.399 kb on + strand, within GFF2178at 25.400 kb on - strand, within GFF2178at 25.750 kb on + strandat 25.779 kb on + strandat 25.793 kb on + strandat 25.795 kb on + strandat 25.796 kb on - strandat 25.820 kb on - strand, within GFF2179at 25.820 kb on - strand, within GFF2179at 25.820 kb on - strand, within GFF2179at 25.824 kb on - strand, within GFF2179at 25.967 kb on - strand, within GFF2179at 26.165 kb on - strand, within GFF2179at 26.248 kb on + strand, within GFF2179at 26.249 kb on - strand, within GFF2179at 26.295 kb on + strandat 26.295 kb on + strandat 26.296 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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25,155 + GFF2177 0.79 +0.2
25,399 + GFF2178 0.26 -1.0
25,400 - GFF2178 0.27 +0.5
25,750 + -1.1
25,779 + +0.2
25,793 + -0.3
25,795 + +0.2
25,796 - -0.3
25,820 - GFF2179 0.13 -0.8
25,820 - GFF2179 0.13 +0.0
25,820 - GFF2179 0.13 -0.1
25,824 - GFF2179 0.14 -1.0
25,967 - GFF2179 0.39 -1.0
26,165 - GFF2179 0.73 -1.2
26,248 + GFF2179 0.88 -0.4
26,249 - GFF2179 0.88 -1.0
26,295 + +0.1
26,295 + -0.1
26,296 - -0.1

Or see this region's nucleotide sequence