Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF184 and GFF185 are separated by 94 nucleotides GFF185 and GFF186 are separated by 82 nucleotides GFF186 and GFF187 are separated by 9 nucleotides
GFF184 - Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70), at 179,100 to 179,759
GFF184
GFF185 - DnaJ-like protein DjlA, at 179,854 to 180,666
GFF185
GFF186 - FIG01045743: hypothetical protein, at 180,749 to 180,910
GFF186
GFF187 - Outer membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor, at 180,920 to 183,280
GFF187
Position (kb) 180
181 Strain fitness (log2 ratio) -1
0
1
2 at 179.881 kb on + strand at 180.203 kb on + strand, within GFF185 at 180.204 kb on - strand, within GFF185 at 180.222 kb on + strand, within GFF185 at 180.223 kb on - strand, within GFF185 at 180.230 kb on - strand, within GFF185 at 180.334 kb on + strand, within GFF185 at 180.342 kb on - strand, within GFF185 at 180.345 kb on + strand, within GFF185 at 180.346 kb on - strand, within GFF185 at 180.473 kb on - strand, within GFF185 at 180.473 kb on - strand, within GFF185 at 180.574 kb on - strand, within GFF185 at 180.667 kb on - strand at 180.667 kb on - strand at 180.714 kb on + strand at 180.767 kb on - strand, within GFF186 at 180.781 kb on + strand, within GFF186 at 180.781 kb on + strand, within GFF186 at 180.782 kb on - strand, within GFF186 at 180.782 kb on - strand, within GFF186 at 180.856 kb on + strand, within GFF186 at 180.856 kb on + strand, within GFF186
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 179,881 + -1.6 180,203 + GFF185 0.43 -0.4 180,204 - GFF185 0.43 -0.5 180,222 + GFF185 0.45 -0.5 180,223 - GFF185 0.45 -0.8 180,230 - GFF185 0.46 +0.3 180,334 + GFF185 0.59 -0.4 180,342 - GFF185 0.60 -1.0 180,345 + GFF185 0.60 -0.4 180,346 - GFF185 0.61 -0.6 180,473 - GFF185 0.76 +0.3 180,473 - GFF185 0.76 -0.5 180,574 - GFF185 0.89 -0.6 180,667 - +0.1 180,667 - -0.4 180,714 + +0.0 180,767 - GFF186 0.11 -0.7 180,781 + GFF186 0.20 +0.1 180,781 + GFF186 0.20 -0.9 180,782 - GFF186 0.20 -0.5 180,782 - GFF186 0.20 -1.1 180,856 + GFF186 0.66 -0.3 180,856 + GFF186 0.66 +2.7
Or see this region's nucleotide sequence