Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1569 and GFF1570 are separated by 21 nucleotides GFF1570 and GFF1571 are separated by 99 nucleotides GFF1571 and GFF1572 are separated by 182 nucleotides GFF1572 and GFF1573 are separated by 9 nucleotides
GFF1569 - CsiR, transcriptional repressor of CsiD, at 32,634 to 33,311
GFF1569
GFF1570 - FIG00638941: hypothetical protein, at 33,333 to 33,782
GFF1570
GFF1571 - FIG00638334: hypothetical protein, at 33,882 to 34,040
GFF1571
GFF1572 - Transcriptional regulator, ArsR family, at 34,223 to 34,522
GFF1572
GFF1573 - Rhodanese-related sulfurtransferases, at 34,532 to 35,059
GFF1573
Position (kb) 33
34
35 Strain fitness (log2 ratio) -2
-1
0
1 at 32.894 kb on + strand, within GFF1569 at 32.972 kb on + strand, within GFF1569 at 32.973 kb on - strand, within GFF1569 at 33.012 kb on - strand, within GFF1569 at 33.088 kb on + strand, within GFF1569 at 33.180 kb on - strand, within GFF1569 at 33.884 kb on - strand at 33.936 kb on + strand, within GFF1571 at 34.302 kb on + strand, within GFF1572 at 34.315 kb on + strand, within GFF1572 at 34.316 kb on - strand, within GFF1572 at 34.512 kb on - strand at 34.546 kb on - strand at 34.619 kb on + strand, within GFF1573 at 34.762 kb on - strand, within GFF1573 at 34.931 kb on - strand, within GFF1573 at 34.931 kb on - strand, within GFF1573 at 35.023 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 32,894 + GFF1569 0.38 -0.0 32,972 + GFF1569 0.50 +0.1 32,973 - GFF1569 0.50 +0.7 33,012 - GFF1569 0.56 +0.3 33,088 + GFF1569 0.67 +0.6 33,180 - GFF1569 0.81 -0.3 33,884 - -0.0 33,936 + GFF1571 0.34 -1.5 34,302 + GFF1572 0.26 -0.0 34,315 + GFF1572 0.31 +0.2 34,316 - GFF1572 0.31 +0.5 34,512 - -0.0 34,546 - +1.3 34,619 + GFF1573 0.16 -2.1 34,762 - GFF1573 0.44 -0.1 34,931 - GFF1573 0.76 +0.1 34,931 - GFF1573 0.76 -1.6 35,023 - -0.8
Or see this region's nucleotide sequence