Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1567 and GFF1568 are separated by 129 nucleotides GFF1568 and GFF1569 are separated by 41 nucleotides GFF1569 and GFF1570 are separated by 21 nucleotides
GFF1567 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 29,779 to 31,062
GFF1567
GFF1568 - gamma-aminobutyrate (GABA) permease, at 31,192 to 32,592
GFF1568
GFF1569 - CsiR, transcriptional repressor of CsiD, at 32,634 to 33,311
GFF1569
GFF1570 - FIG00638941: hypothetical protein, at 33,333 to 33,782
GFF1570
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 30.208 kb on + strand, within GFF1567 at 30.209 kb on - strand, within GFF1567 at 30.209 kb on - strand, within GFF1567 at 30.277 kb on + strand, within GFF1567 at 30.461 kb on + strand, within GFF1567 at 30.481 kb on - strand, within GFF1567 at 30.564 kb on + strand, within GFF1567 at 30.565 kb on - strand, within GFF1567 at 30.753 kb on + strand, within GFF1567 at 30.955 kb on + strand at 30.959 kb on - strand at 31.060 kb on + strand at 31.061 kb on - strand at 31.061 kb on - strand at 31.092 kb on + strand at 31.093 kb on - strand at 31.093 kb on - strand at 31.140 kb on - strand at 31.178 kb on - strand at 31.188 kb on + strand at 31.342 kb on + strand, within GFF1568 at 31.432 kb on + strand, within GFF1568 at 31.467 kb on + strand, within GFF1568 at 31.482 kb on - strand, within GFF1568 at 31.483 kb on + strand, within GFF1568 at 31.484 kb on - strand, within GFF1568 at 31.574 kb on + strand, within GFF1568 at 31.591 kb on - strand, within GFF1568 at 31.616 kb on + strand, within GFF1568 at 31.633 kb on + strand, within GFF1568 at 31.665 kb on + strand, within GFF1568 at 31.666 kb on - strand, within GFF1568 at 31.666 kb on - strand, within GFF1568 at 31.666 kb on - strand, within GFF1568 at 31.729 kb on + strand, within GFF1568 at 31.833 kb on + strand, within GFF1568 at 31.852 kb on - strand, within GFF1568 at 31.854 kb on + strand, within GFF1568 at 31.854 kb on + strand, within GFF1568 at 31.854 kb on + strand, within GFF1568 at 31.855 kb on - strand, within GFF1568 at 31.891 kb on - strand, within GFF1568 at 31.891 kb on - strand, within GFF1568 at 31.915 kb on - strand, within GFF1568 at 31.936 kb on + strand, within GFF1568 at 32.105 kb on + strand, within GFF1568 at 32.105 kb on + strand, within GFF1568 at 32.106 kb on - strand, within GFF1568 at 32.107 kb on + strand, within GFF1568 at 32.107 kb on + strand, within GFF1568 at 32.108 kb on - strand, within GFF1568 at 32.108 kb on - strand, within GFF1568 at 32.110 kb on - strand, within GFF1568 at 32.110 kb on - strand, within GFF1568 at 32.128 kb on + strand, within GFF1568 at 32.129 kb on - strand, within GFF1568 at 32.249 kb on - strand, within GFF1568 at 32.457 kb on - strand at 32.478 kb on - strand at 32.513 kb on + strand at 32.514 kb on - strand at 32.514 kb on - strand at 32.514 kb on - strand at 32.514 kb on - strand at 32.516 kb on + strand at 32.517 kb on - strand at 32.520 kb on + strand at 32.520 kb on + strand at 32.521 kb on - strand at 32.521 kb on - strand at 32.556 kb on - strand at 32.595 kb on - strand at 32.662 kb on + strand at 32.662 kb on + strand at 32.663 kb on - strand at 32.663 kb on - strand at 32.707 kb on - strand, within GFF1569 at 32.737 kb on + strand, within GFF1569 at 32.894 kb on + strand, within GFF1569 at 32.972 kb on + strand, within GFF1569 at 32.973 kb on - strand, within GFF1569 at 33.012 kb on - strand, within GFF1569 at 33.088 kb on + strand, within GFF1569 at 33.180 kb on - strand, within GFF1569
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 30,208 + GFF1567 0.33 -0.2 30,209 - GFF1567 0.33 +0.2 30,209 - GFF1567 0.33 -0.1 30,277 + GFF1567 0.39 +1.1 30,461 + GFF1567 0.53 +0.0 30,481 - GFF1567 0.55 +0.0 30,564 + GFF1567 0.61 -0.1 30,565 - GFF1567 0.61 +0.8 30,753 + GFF1567 0.76 -0.2 30,955 + -1.5 30,959 - +0.1 31,060 + +0.8 31,061 - -0.2 31,061 - -0.2 31,092 + +0.1 31,093 - +0.1 31,093 - +0.0 31,140 - +0.0 31,178 - -0.7 31,188 + -0.1 31,342 + GFF1568 0.11 +0.0 31,432 + GFF1568 0.17 -0.2 31,467 + GFF1568 0.20 -2.9 31,482 - GFF1568 0.21 +0.7 31,483 + GFF1568 0.21 +0.1 31,484 - GFF1568 0.21 +0.0 31,574 + GFF1568 0.27 -0.3 31,591 - GFF1568 0.28 +0.1 31,616 + GFF1568 0.30 +0.2 31,633 + GFF1568 0.31 +0.8 31,665 + GFF1568 0.34 +1.3 31,666 - GFF1568 0.34 +0.4 31,666 - GFF1568 0.34 -0.0 31,666 - GFF1568 0.34 +0.2 31,729 + GFF1568 0.38 +0.6 31,833 + GFF1568 0.46 +0.2 31,852 - GFF1568 0.47 +0.5 31,854 + GFF1568 0.47 +0.4 31,854 + GFF1568 0.47 +0.3 31,854 + GFF1568 0.47 +1.0 31,855 - GFF1568 0.47 -0.1 31,891 - GFF1568 0.50 +0.2 31,891 - GFF1568 0.50 +1.0 31,915 - GFF1568 0.52 -0.7 31,936 + GFF1568 0.53 +0.1 32,105 + GFF1568 0.65 -1.0 32,105 + GFF1568 0.65 +0.7 32,106 - GFF1568 0.65 +0.3 32,107 + GFF1568 0.65 -0.3 32,107 + GFF1568 0.65 -0.3 32,108 - GFF1568 0.65 -1.3 32,108 - GFF1568 0.65 +0.8 32,110 - GFF1568 0.66 +0.2 32,110 - GFF1568 0.66 +0.3 32,128 + GFF1568 0.67 +0.0 32,129 - GFF1568 0.67 -0.1 32,249 - GFF1568 0.75 -0.8 32,457 - +0.2 32,478 - +0.5 32,513 + +0.3 32,514 - -1.1 32,514 - -0.4 32,514 - +1.2 32,514 - +0.1 32,516 + +0.4 32,517 - -0.2 32,520 + -0.3 32,520 + -0.5 32,521 - -0.2 32,521 - +0.4 32,556 - +0.4 32,595 - +1.3 32,662 + -0.3 32,662 + +0.1 32,663 - -0.3 32,663 - +0.5 32,707 - GFF1569 0.11 +0.1 32,737 + GFF1569 0.15 -0.0 32,894 + GFF1569 0.38 -0.0 32,972 + GFF1569 0.50 +0.1 32,973 - GFF1569 0.50 +0.7 33,012 - GFF1569 0.56 +0.3 33,088 + GFF1569 0.67 +0.6 33,180 - GFF1569 0.81 -0.3
Or see this region's nucleotide sequence