Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1359 and GFF1360 are separated by 75 nucleotides GFF1360 and GFF1361 are separated by 207 nucleotides GFF1361 and GFF1362 are separated by 46 nucleotides
GFF1359 - Sodium-dependent phosphate transporter, at 12,417 to 14,048
GFF1359
GFF1360 - Alpha-aspartyl dipeptidase Peptidase E (EC 3.4.13.21), at 14,124 to 14,813
GFF1360
GFF1361 - Nucleoprotein/polynucleotide-associated enzyme, at 15,021 to 15,560
GFF1361
GFF1362 - Ribosomal large subunit pseudouridine synthase F (EC 4.2.1.70), at 15,607 to 16,476
GFF1362
Position (kb) 14
15 Strain fitness (log2 ratio) -2
-1
0
1 at 13.145 kb on - strand, within GFF1359 at 13.145 kb on - strand, within GFF1359 at 13.261 kb on - strand, within GFF1359 at 13.566 kb on + strand, within GFF1359 at 13.775 kb on + strand, within GFF1359 at 13.865 kb on + strand, within GFF1359 at 13.973 kb on + strand at 13.973 kb on + strand at 13.998 kb on + strand at 14.000 kb on + strand at 14.043 kb on + strand at 14.047 kb on - strand at 14.112 kb on + strand at 14.291 kb on + strand, within GFF1360 at 14.336 kb on + strand, within GFF1360 at 14.467 kb on + strand, within GFF1360 at 14.467 kb on + strand, within GFF1360 at 14.485 kb on - strand, within GFF1360 at 14.535 kb on + strand, within GFF1360 at 14.705 kb on + strand, within GFF1360 at 14.815 kb on - strand at 14.850 kb on + strand at 14.851 kb on - strand at 14.870 kb on + strand at 14.878 kb on + strand at 14.900 kb on - strand at 14.997 kb on + strand at 15.001 kb on + strand at 15.038 kb on - strand at 15.052 kb on + strand at 15.414 kb on + strand, within GFF1361 at 15.559 kb on - strand at 15.559 kb on - strand at 15.583 kb on - strand at 15.606 kb on + strand at 15.640 kb on + strand at 15.640 kb on + strand at 15.640 kb on + strand at 15.642 kb on + strand at 15.806 kb on + strand, within GFF1362 at 15.806 kb on + strand, within GFF1362
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 13,145 - GFF1359 0.45 -0.5 13,145 - GFF1359 0.45 +0.6 13,261 - GFF1359 0.52 +1.6 13,566 + GFF1359 0.70 -0.1 13,775 + GFF1359 0.83 +1.2 13,865 + GFF1359 0.89 -0.2 13,973 + +0.1 13,973 + +0.0 13,998 + +0.4 14,000 + +0.2 14,043 + +0.3 14,047 - +0.5 14,112 + +0.2 14,291 + GFF1360 0.24 -0.2 14,336 + GFF1360 0.31 +0.5 14,467 + GFF1360 0.50 +0.3 14,467 + GFF1360 0.50 -2.1 14,485 - GFF1360 0.52 +0.6 14,535 + GFF1360 0.60 -1.0 14,705 + GFF1360 0.84 +1.2 14,815 - -0.9 14,850 + -0.9 14,851 - +0.1 14,870 + +0.1 14,878 + -0.0 14,900 - +0.4 14,997 + -0.8 15,001 + +0.5 15,038 - +0.6 15,052 + +0.4 15,414 + GFF1361 0.73 -2.1 15,559 - -0.1 15,559 - -0.8 15,583 - -0.1 15,606 + -0.1 15,640 + +0.0 15,640 + -0.3 15,640 + +1.2 15,642 + +1.5 15,806 + GFF1362 0.23 +0.2 15,806 + GFF1362 0.23 +0.1
Or see this region's nucleotide sequence