Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1348 and GFF1349 overlap by 10 nucleotides GFF1349 and GFF1350 are separated by 156 nucleotides GFF1350 and GFF1351 are separated by 265 nucleotides
GFF1348 - FIG01046723: hypothetical protein, at 154 to 384
GFF1348
GFF1349 - Putative acetyltransferase, at 375 to 812
GFF1349
GFF1350 - Homoserine O-succinyltransferase (EC 2.3.1.46), at 969 to 1,898
GFF1350
GFF1351 - Malate synthase (EC 2.3.3.9), at 2,164 to 3,768
GFF1351
Position (kb)
0
1
2 Strain fitness (log2 ratio)
-1
0
1
2
3 at 0.090 kb on - strand at 0.270 kb on + strand, within GFF1348 at 0.838 kb on - strand at 0.948 kb on - strand at 0.968 kb on + strand at 1.082 kb on - strand, within GFF1350 at 1.086 kb on - strand, within GFF1350 at 1.086 kb on - strand, within GFF1350 at 1.234 kb on - strand, within GFF1350 at 1.374 kb on - strand, within GFF1350 at 1.419 kb on - strand, within GFF1350 at 1.470 kb on + strand, within GFF1350 at 1.470 kb on + strand, within GFF1350 at 1.549 kb on - strand, within GFF1350 at 1.647 kb on - strand, within GFF1350 at 1.652 kb on + strand, within GFF1350 at 1.652 kb on + strand, within GFF1350 at 1.652 kb on + strand, within GFF1350 at 1.653 kb on - strand, within GFF1350 at 1.680 kb on + strand, within GFF1350 at 1.755 kb on + strand, within GFF1350 at 1.824 kb on - strand at 1.842 kb on + strand at 2.139 kb on - strand at 2.199 kb on - strand at 2.466 kb on + strand, within GFF1351 at 2.467 kb on - strand, within GFF1351 at 2.479 kb on - strand, within GFF1351 at 2.553 kb on + strand, within GFF1351 at 2.602 kb on + strand, within GFF1351 at 2.602 kb on + strand, within GFF1351 at 2.603 kb on - strand, within GFF1351 at 2.603 kb on - strand, within GFF1351 at 2.603 kb on - strand, within GFF1351 at 2.666 kb on + strand, within GFF1351 at 2.667 kb on - strand, within GFF1351 at 2.676 kb on - strand, within GFF1351 at 2.697 kb on + strand, within GFF1351 at 2.698 kb on - strand, within GFF1351 at 2.762 kb on - strand, within GFF1351 at 2.762 kb on - strand, within GFF1351 at 2.784 kb on + strand, within GFF1351 at 2.800 kb on + strand, within GFF1351
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 90 - +0.2 270 + GFF1348 0.50 +0.5 838 - -0.3 948 - +3.6 968 + +0.1 1,082 - GFF1350 0.12 +0.3 1,086 - GFF1350 0.13 +0.9 1,086 - GFF1350 0.13 +0.3 1,234 - GFF1350 0.28 -1.1 1,374 - GFF1350 0.44 +0.7 1,419 - GFF1350 0.48 -0.1 1,470 + GFF1350 0.54 +0.8 1,470 + GFF1350 0.54 +0.2 1,549 - GFF1350 0.62 -0.2 1,647 - GFF1350 0.73 +1.0 1,652 + GFF1350 0.73 -1.1 1,652 + GFF1350 0.73 -0.6 1,652 + GFF1350 0.73 +0.7 1,653 - GFF1350 0.74 -0.2 1,680 + GFF1350 0.76 +0.7 1,755 + GFF1350 0.85 -0.3 1,824 - +0.3 1,842 + -0.2 2,139 - -0.9 2,199 - +0.6 2,466 + GFF1351 0.19 -0.0 2,467 - GFF1351 0.19 -0.5 2,479 - GFF1351 0.20 +2.1 2,553 + GFF1351 0.24 +0.2 2,602 + GFF1351 0.27 -0.1 2,602 + GFF1351 0.27 -0.0 2,603 - GFF1351 0.27 +0.1 2,603 - GFF1351 0.27 -1.3 2,603 - GFF1351 0.27 -0.1 2,666 + GFF1351 0.31 +0.3 2,667 - GFF1351 0.31 -0.1 2,676 - GFF1351 0.32 -0.3 2,697 + GFF1351 0.33 -0.3 2,698 - GFF1351 0.33 +0.1 2,762 - GFF1351 0.37 +0.1 2,762 - GFF1351 0.37 +0.3 2,784 + GFF1351 0.39 +0.0 2,800 + GFF1351 0.40 +1.1
Or see this region's nucleotide sequence