Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4858 and GFF4859 are separated by 31 nucleotides GFF4859 and GFF4860 are separated by 100 nucleotides
GFF4858 - Fucose permease, at 156,837 to 158,153
GFF4858
GFF4859 - L-fucose isomerase (EC 5.3.1.25), at 158,185 to 159,960
GFF4859
GFF4860 - L-fuculokinase (EC 2.7.1.51), at 160,061 to 161,479
GFF4860
Position (kb) 158
159
160 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 157.437 kb on - strand, within GFF4858 at 157.470 kb on + strand, within GFF4858 at 157.495 kb on + strand, within GFF4858 at 157.496 kb on - strand, within GFF4858 at 157.498 kb on + strand, within GFF4858 at 157.499 kb on - strand, within GFF4858 at 157.645 kb on - strand, within GFF4858 at 157.645 kb on - strand, within GFF4858 at 157.880 kb on + strand, within GFF4858 at 157.976 kb on + strand, within GFF4858 at 157.977 kb on - strand, within GFF4858 at 157.980 kb on + strand, within GFF4858 at 157.980 kb on + strand, within GFF4858 at 158.458 kb on + strand, within GFF4859 at 158.515 kb on - strand, within GFF4859 at 158.543 kb on - strand, within GFF4859 at 158.629 kb on - strand, within GFF4859 at 158.767 kb on - strand, within GFF4859 at 158.930 kb on - strand, within GFF4859 at 158.992 kb on - strand, within GFF4859 at 159.038 kb on + strand, within GFF4859 at 159.059 kb on - strand, within GFF4859 at 159.145 kb on - strand, within GFF4859 at 159.277 kb on + strand, within GFF4859 at 159.345 kb on + strand, within GFF4859 at 159.345 kb on + strand, within GFF4859 at 159.357 kb on + strand, within GFF4859 at 159.357 kb on + strand, within GFF4859 at 159.390 kb on + strand, within GFF4859 at 159.503 kb on - strand, within GFF4859 at 159.676 kb on - strand, within GFF4859 at 159.684 kb on + strand, within GFF4859 at 159.684 kb on + strand, within GFF4859 at 159.684 kb on + strand, within GFF4859 at 159.688 kb on - strand, within GFF4859 at 159.791 kb on + strand at 159.808 kb on - strand at 159.837 kb on - strand at 159.840 kb on + strand at 159.840 kb on + strand at 159.840 kb on + strand at 159.841 kb on - strand at 159.841 kb on - strand at 159.841 kb on - strand at 159.841 kb on - strand at 159.841 kb on - strand at 159.949 kb on + strand at 160.014 kb on - strand at 160.057 kb on - strand at 160.061 kb on - strand at 160.300 kb on + strand, within GFF4860 at 160.300 kb on + strand, within GFF4860 at 160.301 kb on - strand, within GFF4860 at 160.411 kb on + strand, within GFF4860 at 160.537 kb on + strand, within GFF4860 at 160.592 kb on - strand, within GFF4860 at 160.946 kb on + strand, within GFF4860 at 160.946 kb on + strand, within GFF4860 at 160.948 kb on + strand, within GFF4860 at 160.948 kb on + strand, within GFF4860
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 157,437 - GFF4858 0.46 +0.1 157,470 + GFF4858 0.48 +0.1 157,495 + GFF4858 0.50 +0.3 157,496 - GFF4858 0.50 +0.3 157,498 + GFF4858 0.50 -0.8 157,499 - GFF4858 0.50 +1.3 157,645 - GFF4858 0.61 +0.9 157,645 - GFF4858 0.61 +0.7 157,880 + GFF4858 0.79 +0.2 157,976 + GFF4858 0.86 -1.7 157,977 - GFF4858 0.87 +0.4 157,980 + GFF4858 0.87 +0.1 157,980 + GFF4858 0.87 +0.3 158,458 + GFF4859 0.15 -3.5 158,515 - GFF4859 0.19 -1.7 158,543 - GFF4859 0.20 +1.7 158,629 - GFF4859 0.25 +2.4 158,767 - GFF4859 0.33 +1.7 158,930 - GFF4859 0.42 +0.2 158,992 - GFF4859 0.45 +0.1 159,038 + GFF4859 0.48 -0.4 159,059 - GFF4859 0.49 +0.6 159,145 - GFF4859 0.54 +0.0 159,277 + GFF4859 0.61 +0.6 159,345 + GFF4859 0.65 +0.0 159,345 + GFF4859 0.65 +0.5 159,357 + GFF4859 0.66 +0.2 159,357 + GFF4859 0.66 +0.6 159,390 + GFF4859 0.68 +1.2 159,503 - GFF4859 0.74 -0.2 159,676 - GFF4859 0.84 -0.9 159,684 + GFF4859 0.84 -0.1 159,684 + GFF4859 0.84 -0.1 159,684 + GFF4859 0.84 +2.1 159,688 - GFF4859 0.85 -1.1 159,791 + -0.2 159,808 - -0.7 159,837 - +1.5 159,840 + -0.2 159,840 + +0.1 159,840 + -0.2 159,841 - +0.4 159,841 - +0.2 159,841 - +0.0 159,841 - +0.1 159,841 - -0.4 159,949 + -0.5 160,014 - +1.1 160,057 - +0.4 160,061 - +0.2 160,300 + GFF4860 0.17 +0.9 160,300 + GFF4860 0.17 +0.0 160,301 - GFF4860 0.17 +0.2 160,411 + GFF4860 0.25 +0.5 160,537 + GFF4860 0.34 -0.6 160,592 - GFF4860 0.37 -0.4 160,946 + GFF4860 0.62 -0.2 160,946 + GFF4860 0.62 +0.5 160,948 + GFF4860 0.63 -0.5 160,948 + GFF4860 0.63 -0.8
Or see this region's nucleotide sequence