Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4751

Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4750 and GFF4751 are separated by 27 nucleotidesGFF4751 and GFF4752 are separated by 2 nucleotidesGFF4752 and GFF4753 are separated by 137 nucleotides GFF4750 - Type III secretion negative modulator of injection (YopK,YopQ,controls size of translocator pore), at 57,640 to 58,869 GFF4750 GFF4751 - cell invasion protein SipB, at 58,897 to 60,678 GFF4751 GFF4752 - Type III secretion chaperone protein for YopD (SycD), at 60,681 to 61,178 GFF4752 GFF4753 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 61,316 to 62,386 GFF4753 Position (kb) 58 59 60 61Strain fitness (log2 ratio) -1 0 1at 57.933 kb on + strand, within GFF4750at 57.934 kb on - strand, within GFF4750at 57.934 kb on - strand, within GFF4750at 58.078 kb on - strand, within GFF4750at 58.128 kb on + strand, within GFF4750at 58.129 kb on - strand, within GFF4750at 58.177 kb on - strand, within GFF4750at 58.177 kb on - strand, within GFF4750at 58.200 kb on + strand, within GFF4750at 58.203 kb on + strand, within GFF4750at 58.228 kb on - strand, within GFF4750at 58.281 kb on + strand, within GFF4750at 58.412 kb on - strand, within GFF4750at 58.468 kb on - strand, within GFF4750at 58.681 kb on + strand, within GFF4750at 59.169 kb on + strand, within GFF4751at 59.182 kb on - strand, within GFF4751at 59.182 kb on - strand, within GFF4751at 59.185 kb on + strand, within GFF4751at 59.197 kb on - strand, within GFF4751at 59.251 kb on - strand, within GFF4751at 59.704 kb on + strand, within GFF4751at 59.739 kb on + strand, within GFF4751at 60.048 kb on + strand, within GFF4751at 60.069 kb on + strand, within GFF4751at 60.070 kb on - strand, within GFF4751at 60.070 kb on - strand, within GFF4751at 60.073 kb on + strand, within GFF4751at 60.116 kb on - strand, within GFF4751at 60.395 kb on - strand, within GFF4751at 60.403 kb on + strand, within GFF4751at 60.403 kb on + strand, within GFF4751at 60.403 kb on + strand, within GFF4751at 60.404 kb on - strand, within GFF4751at 60.640 kb on - strandat 60.821 kb on + strand, within GFF4752at 60.939 kb on + strand, within GFF4752at 60.940 kb on - strand, within GFF4752at 61.060 kb on - strand, within GFF4752at 61.074 kb on - strand, within GFF4752at 61.164 kb on - strandat 61.184 kb on - strandat 61.210 kb on + strandat 61.301 kb on - strandat 61.309 kb on - strandat 61.510 kb on - strand, within GFF4753

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI
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57,933 + GFF4750 0.24 +1.6
57,934 - GFF4750 0.24 +0.5
57,934 - GFF4750 0.24 -0.5
58,078 - GFF4750 0.36 +0.4
58,128 + GFF4750 0.40 -0.4
58,129 - GFF4750 0.40 +0.0
58,177 - GFF4750 0.44 -1.1
58,177 - GFF4750 0.44 +0.1
58,200 + GFF4750 0.46 -0.5
58,203 + GFF4750 0.46 -0.0
58,228 - GFF4750 0.48 -0.4
58,281 + GFF4750 0.52 +0.1
58,412 - GFF4750 0.63 -0.5
58,468 - GFF4750 0.67 +0.1
58,681 + GFF4750 0.85 +0.5
59,169 + GFF4751 0.15 +0.5
59,182 - GFF4751 0.16 +0.7
59,182 - GFF4751 0.16 +0.2
59,185 + GFF4751 0.16 -0.2
59,197 - GFF4751 0.17 -0.8
59,251 - GFF4751 0.20 -0.5
59,704 + GFF4751 0.45 -0.0
59,739 + GFF4751 0.47 +1.3
60,048 + GFF4751 0.65 -0.4
60,069 + GFF4751 0.66 -0.4
60,070 - GFF4751 0.66 -0.1
60,070 - GFF4751 0.66 -0.4
60,073 + GFF4751 0.66 -1.1
60,116 - GFF4751 0.68 -1.4
60,395 - GFF4751 0.84 -0.4
60,403 + GFF4751 0.85 -0.3
60,403 + GFF4751 0.85 -0.6
60,403 + GFF4751 0.85 -0.4
60,404 - GFF4751 0.85 -0.4
60,640 - -1.0
60,821 + GFF4752 0.28 -0.5
60,939 + GFF4752 0.52 -0.8
60,940 - GFF4752 0.52 -0.1
61,060 - GFF4752 0.76 -0.3
61,074 - GFF4752 0.79 +0.8
61,164 - -1.3
61,184 - -0.1
61,210 + +0.1
61,301 - -0.1
61,309 - -0.8
61,510 - GFF4753 0.18 -0.1

Or see this region's nucleotide sequence