Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4750 and GFF4751 are separated by 27 nucleotides GFF4751 and GFF4752 are separated by 2 nucleotides GFF4752 and GFF4753 are separated by 137 nucleotides
GFF4750 - Type III secretion negative modulator of injection (YopK,YopQ,controls size of translocator pore), at 57,640 to 58,869
GFF4750
GFF4751 - cell invasion protein SipB, at 58,897 to 60,678
GFF4751
GFF4752 - Type III secretion chaperone protein for YopD (SycD), at 60,681 to 61,178
GFF4752
GFF4753 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 61,316 to 62,386
GFF4753
Position (kb)
58
59
60
61 Strain fitness (log2 ratio)
-1
0
1 at 57.933 kb on + strand, within GFF4750 at 57.934 kb on - strand, within GFF4750 at 57.934 kb on - strand, within GFF4750 at 58.078 kb on - strand, within GFF4750 at 58.128 kb on + strand, within GFF4750 at 58.129 kb on - strand, within GFF4750 at 58.177 kb on - strand, within GFF4750 at 58.177 kb on - strand, within GFF4750 at 58.200 kb on + strand, within GFF4750 at 58.203 kb on + strand, within GFF4750 at 58.228 kb on - strand, within GFF4750 at 58.281 kb on + strand, within GFF4750 at 58.412 kb on - strand, within GFF4750 at 58.468 kb on - strand, within GFF4750 at 58.681 kb on + strand, within GFF4750 at 59.169 kb on + strand, within GFF4751 at 59.182 kb on - strand, within GFF4751 at 59.182 kb on - strand, within GFF4751 at 59.185 kb on + strand, within GFF4751 at 59.197 kb on - strand, within GFF4751 at 59.251 kb on - strand, within GFF4751 at 59.704 kb on + strand, within GFF4751 at 59.739 kb on + strand, within GFF4751 at 60.048 kb on + strand, within GFF4751 at 60.069 kb on + strand, within GFF4751 at 60.070 kb on - strand, within GFF4751 at 60.070 kb on - strand, within GFF4751 at 60.073 kb on + strand, within GFF4751 at 60.116 kb on - strand, within GFF4751 at 60.395 kb on - strand, within GFF4751 at 60.403 kb on + strand, within GFF4751 at 60.403 kb on + strand, within GFF4751 at 60.403 kb on + strand, within GFF4751 at 60.404 kb on - strand, within GFF4751 at 60.640 kb on - strand at 60.821 kb on + strand, within GFF4752 at 60.939 kb on + strand, within GFF4752 at 60.940 kb on - strand, within GFF4752 at 61.060 kb on - strand, within GFF4752 at 61.074 kb on - strand, within GFF4752 at 61.164 kb on - strand at 61.184 kb on - strand at 61.210 kb on + strand at 61.301 kb on - strand at 61.309 kb on - strand at 61.510 kb on - strand, within GFF4753
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 57,933 + GFF4750 0.24 +1.6 57,934 - GFF4750 0.24 +0.5 57,934 - GFF4750 0.24 -0.5 58,078 - GFF4750 0.36 +0.4 58,128 + GFF4750 0.40 -0.4 58,129 - GFF4750 0.40 +0.0 58,177 - GFF4750 0.44 -1.1 58,177 - GFF4750 0.44 +0.1 58,200 + GFF4750 0.46 -0.5 58,203 + GFF4750 0.46 -0.0 58,228 - GFF4750 0.48 -0.4 58,281 + GFF4750 0.52 +0.1 58,412 - GFF4750 0.63 -0.5 58,468 - GFF4750 0.67 +0.1 58,681 + GFF4750 0.85 +0.5 59,169 + GFF4751 0.15 +0.5 59,182 - GFF4751 0.16 +0.7 59,182 - GFF4751 0.16 +0.2 59,185 + GFF4751 0.16 -0.2 59,197 - GFF4751 0.17 -0.8 59,251 - GFF4751 0.20 -0.5 59,704 + GFF4751 0.45 -0.0 59,739 + GFF4751 0.47 +1.3 60,048 + GFF4751 0.65 -0.4 60,069 + GFF4751 0.66 -0.4 60,070 - GFF4751 0.66 -0.1 60,070 - GFF4751 0.66 -0.4 60,073 + GFF4751 0.66 -1.1 60,116 - GFF4751 0.68 -1.4 60,395 - GFF4751 0.84 -0.4 60,403 + GFF4751 0.85 -0.3 60,403 + GFF4751 0.85 -0.6 60,403 + GFF4751 0.85 -0.4 60,404 - GFF4751 0.85 -0.4 60,640 - -1.0 60,821 + GFF4752 0.28 -0.5 60,939 + GFF4752 0.52 -0.8 60,940 - GFF4752 0.52 -0.1 61,060 - GFF4752 0.76 -0.3 61,074 - GFF4752 0.79 +0.8 61,164 - -1.3 61,184 - -0.1 61,210 + +0.1 61,301 - -0.1 61,309 - -0.8 61,510 - GFF4753 0.18 -0.1
Or see this region's nucleotide sequence