Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF470 and GFF638 are separated by 90 nucleotides GFF638 and GFF471 are separated by 159 nucleotides GFF471 and GFF472 overlap by 17 nucleotides GFF472 and GFF473 are separated by 52 nucleotides
GFF470 - Phosphoethanolamine transferase specific for the outer Kdo residue of lipopolysaccharide, at 190,811 to 192,502
GFF470
GFF638 - tRNA-Pro-CGG, at 192,593 to 192,669
GFF638
GFF471 - Putative lacI-family transcriptional regulator, at 192,829 to 193,836
GFF471
GFF472 - hypothetical protein, at 193,820 to 193,933
GFF472
GFF473 - Putative PQQ enzyme repeat, at 193,986 to 195,110
GFF473
Position (kb) 192
193
194 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 191.840 kb on + strand, within GFF470 at 191.841 kb on - strand, within GFF470 at 191.841 kb on - strand, within GFF470 at 191.863 kb on + strand, within GFF470 at 191.864 kb on - strand, within GFF470 at 191.870 kb on + strand, within GFF470 at 192.017 kb on + strand, within GFF470 at 192.065 kb on - strand, within GFF470 at 192.110 kb on + strand, within GFF470 at 192.111 kb on - strand, within GFF470 at 192.116 kb on - strand, within GFF470 at 192.128 kb on + strand, within GFF470 at 192.128 kb on + strand, within GFF470 at 192.128 kb on + strand, within GFF470 at 192.128 kb on + strand, within GFF470 at 192.128 kb on + strand, within GFF470 at 192.142 kb on + strand, within GFF470 at 192.305 kb on - strand, within GFF470 at 192.393 kb on + strand at 192.393 kb on + strand at 192.393 kb on + strand at 192.393 kb on + strand at 192.394 kb on - strand at 192.394 kb on - strand at 192.394 kb on - strand at 192.396 kb on - strand at 192.424 kb on + strand at 192.424 kb on + strand at 192.424 kb on + strand at 192.425 kb on - strand at 192.500 kb on + strand at 192.577 kb on - strand at 192.810 kb on - strand at 193.129 kb on + strand, within GFF471 at 193.130 kb on - strand, within GFF471 at 193.130 kb on - strand, within GFF471 at 193.130 kb on - strand, within GFF471 at 193.274 kb on - strand, within GFF471 at 193.572 kb on + strand, within GFF471 at 193.573 kb on - strand, within GFF471 at 193.683 kb on - strand, within GFF471 at 193.876 kb on + strand, within GFF472 at 194.043 kb on - strand at 194.043 kb on - strand at 194.047 kb on - strand at 194.047 kb on - strand at 194.047 kb on - strand at 194.079 kb on - strand at 194.116 kb on - strand, within GFF473 at 194.211 kb on + strand, within GFF473 at 194.211 kb on + strand, within GFF473 at 194.280 kb on + strand, within GFF473 at 194.281 kb on - strand, within GFF473 at 194.325 kb on - strand, within GFF473 at 194.325 kb on - strand, within GFF473 at 194.328 kb on + strand, within GFF473 at 194.373 kb on + strand, within GFF473 at 194.374 kb on - strand, within GFF473 at 194.664 kb on - strand, within GFF473
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 191,840 + GFF470 0.61 +0.2 191,841 - GFF470 0.61 +0.9 191,841 - GFF470 0.61 +1.5 191,863 + GFF470 0.62 +0.3 191,864 - GFF470 0.62 +0.3 191,870 + GFF470 0.63 +0.2 192,017 + GFF470 0.71 -0.6 192,065 - GFF470 0.74 -0.4 192,110 + GFF470 0.77 +0.2 192,111 - GFF470 0.77 +0.3 192,116 - GFF470 0.77 +0.3 192,128 + GFF470 0.78 +0.0 192,128 + GFF470 0.78 -0.7 192,128 + GFF470 0.78 +0.2 192,128 + GFF470 0.78 -0.9 192,128 + GFF470 0.78 -0.4 192,142 + GFF470 0.79 +0.1 192,305 - GFF470 0.88 -0.0 192,393 + +0.0 192,393 + -0.4 192,393 + +0.1 192,393 + +4.2 192,394 - +0.0 192,394 - -0.7 192,394 - +0.3 192,396 - -1.3 192,424 + +0.4 192,424 + +0.4 192,424 + +0.5 192,425 - +0.6 192,500 + -0.8 192,577 - +1.6 192,810 - -0.3 193,129 + GFF471 0.30 +0.8 193,130 - GFF471 0.30 -0.8 193,130 - GFF471 0.30 +0.9 193,130 - GFF471 0.30 +0.8 193,274 - GFF471 0.44 +0.5 193,572 + GFF471 0.74 +0.6 193,573 - GFF471 0.74 +0.7 193,683 - GFF471 0.85 -0.5 193,876 + GFF472 0.49 +0.1 194,043 - +0.0 194,043 - -0.4 194,047 - -0.7 194,047 - +0.0 194,047 - -0.3 194,079 - -0.7 194,116 - GFF473 0.12 -0.5 194,211 + GFF473 0.20 +0.0 194,211 + GFF473 0.20 +0.2 194,280 + GFF473 0.26 -0.5 194,281 - GFF473 0.26 -0.3 194,325 - GFF473 0.30 -0.2 194,325 - GFF473 0.30 -0.9 194,328 + GFF473 0.30 -0.1 194,373 + GFF473 0.34 -0.8 194,374 - GFF473 0.34 -1.4 194,664 - GFF473 0.60 +0.1
Or see this region's nucleotide sequence