Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF402 and GFF403 are separated by 77 nucleotides GFF403 and GFF404 are separated by 82 nucleotides GFF404 and GFF405 are separated by 73 nucleotides
GFF402 - Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94), at 117,042 to 118,061
GFF402
GFF403 - Serine acetyltransferase (EC 2.3.1.30), at 118,139 to 118,960
GFF403
GFF404 - D-glucarate permease, at 119,043 to 120,353
GFF404
GFF405 - L-talarate dehydratase @ Galactarate dehydratase (EC 4.2.1.42), at 120,427 to 121,623
GFF405
Position (kb)
119
120
121 Strain fitness (log2 ratio)
-1
0
1 at 118.933 kb on + strand at 118.970 kb on + strand at 119.115 kb on - strand at 119.135 kb on - strand at 119.225 kb on + strand, within GFF404 at 119.225 kb on + strand, within GFF404 at 119.229 kb on + strand, within GFF404 at 119.359 kb on + strand, within GFF404 at 119.393 kb on + strand, within GFF404 at 119.403 kb on - strand, within GFF404 at 119.433 kb on - strand, within GFF404 at 119.560 kb on + strand, within GFF404 at 119.595 kb on - strand, within GFF404 at 119.693 kb on + strand, within GFF404 at 120.052 kb on + strand, within GFF404 at 120.053 kb on - strand, within GFF404 at 120.058 kb on + strand, within GFF404 at 120.058 kb on + strand, within GFF404 at 120.172 kb on + strand, within GFF404 at 120.172 kb on + strand, within GFF404 at 120.240 kb on + strand at 120.342 kb on + strand at 120.358 kb on + strand at 120.654 kb on + strand, within GFF405 at 120.654 kb on + strand, within GFF405 at 120.734 kb on - strand, within GFF405 at 120.811 kb on - strand, within GFF405 at 120.860 kb on - strand, within GFF405 at 120.880 kb on - strand, within GFF405 at 120.880 kb on - strand, within GFF405 at 120.880 kb on - strand, within GFF405 at 121.005 kb on + strand, within GFF405 at 121.104 kb on + strand, within GFF405 at 121.192 kb on - strand, within GFF405 at 121.230 kb on + strand, within GFF405 at 121.231 kb on - strand, within GFF405 at 121.291 kb on + strand, within GFF405 at 121.325 kb on - strand, within GFF405 at 121.337 kb on - strand, within GFF405
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 118,933 + -0.6 118,970 + -0.8 119,115 - -0.6 119,135 - -0.7 119,225 + GFF404 0.14 -1.4 119,225 + GFF404 0.14 +0.6 119,229 + GFF404 0.14 +1.4 119,359 + GFF404 0.24 -1.3 119,393 + GFF404 0.27 -0.6 119,403 - GFF404 0.27 +0.8 119,433 - GFF404 0.30 -0.1 119,560 + GFF404 0.39 -0.5 119,595 - GFF404 0.42 +0.1 119,693 + GFF404 0.50 +0.2 120,052 + GFF404 0.77 -0.4 120,053 - GFF404 0.77 +0.7 120,058 + GFF404 0.77 -0.1 120,058 + GFF404 0.77 +0.6 120,172 + GFF404 0.86 -1.6 120,172 + GFF404 0.86 -0.1 120,240 + +0.5 120,342 + -1.6 120,358 + -0.6 120,654 + GFF405 0.19 -0.0 120,654 + GFF405 0.19 -0.1 120,734 - GFF405 0.26 +0.5 120,811 - GFF405 0.32 -0.9 120,860 - GFF405 0.36 -0.0 120,880 - GFF405 0.38 -0.3 120,880 - GFF405 0.38 -0.3 120,880 - GFF405 0.38 -0.1 121,005 + GFF405 0.48 +0.1 121,104 + GFF405 0.57 +0.5 121,192 - GFF405 0.64 -1.0 121,230 + GFF405 0.67 -0.1 121,231 - GFF405 0.67 +0.2 121,291 + GFF405 0.72 -0.2 121,325 - GFF405 0.75 +0.3 121,337 - GFF405 0.76 +0.2
Or see this region's nucleotide sequence