Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3677 and GFF3678 are separated by 135 nucleotides GFF3678 and GFF3679 overlap by 8 nucleotides GFF3679 and GFF3680 overlap by 20 nucleotides GFF3680 and GFF3681 overlap by 1 nucleotides
GFF3677 - Membrane Protein Functionally coupled to the MukBEF Chromosome Partitioning Mechanism, at 28,094 to 28,870
GFF3677
GFF3678 - S-adenosylmethionine-dependent methyltransferase Functionally Coupled to the MukBEF Chromosome Partitioning Mechanism, at 29,006 to 29,809
GFF3678
GFF3679 - Chromosome partition protein MukF, at 29,802 to 31,124
GFF3679
GFF3680 - Chromosome partition protein MukE, at 31,105 to 31,809
GFF3680
GFF3681 - Chromosome partition protein MukB, at 31,809 to 36,275
GFF3681
Position (kb) 29
30
31
32 Strain fitness (log2 ratio) -2
-1
0
1 at 28.832 kb on + strand at 28.833 kb on - strand at 28.837 kb on + strand at 28.895 kb on + strand at 28.895 kb on + strand at 28.896 kb on - strand at 28.896 kb on - strand at 28.897 kb on + strand at 28.898 kb on - strand at 28.917 kb on + strand at 28.920 kb on + strand at 29.298 kb on + strand, within GFF3678 at 29.400 kb on + strand, within GFF3678 at 29.418 kb on + strand, within GFF3678 at 29.482 kb on + strand, within GFF3678 at 29.645 kb on + strand, within GFF3678
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 28,832 + +0.7 28,833 - +0.1 28,837 + -0.7 28,895 + -0.8 28,895 + -2.5 28,896 - -0.4 28,896 - +1.1 28,897 + -0.3 28,898 - +0.2 28,917 + +0.5 28,920 + +0.1 29,298 + GFF3678 0.36 +0.0 29,400 + GFF3678 0.49 -1.7 29,418 + GFF3678 0.51 -0.3 29,482 + GFF3678 0.59 -0.2 29,645 + GFF3678 0.79 +0.4
Or see this region's nucleotide sequence