Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3557 and GFF3558 are separated by 426 nucleotides GFF3558 and GFF3559 are separated by 8 nucleotides GFF3559 and GFF3560 are separated by 16 nucleotides GFF3560 and GFF3561 overlap by 26 nucleotides
GFF3557 - L-cystine uptake protein TcyP, at 312,595 to 313,941
GFF3557
GFF3558 - Iron binding protein SufA for iron-sulfur cluster assembly, at 314,368 to 314,736
GFF3558
GFF3559 - Iron-sulfur cluster assembly protein SufB, at 314,745 to 316,232
GFF3559
GFF3560 - Iron-sulfur cluster assembly ATPase protein SufC, at 316,249 to 316,995
GFF3560
GFF3561 - Iron-sulfur cluster assembly protein SufD, at 316,970 to 318,241
GFF3561
Position (kb) 314
315
316
317 Strain fitness (log2 ratio) -2
-1
0
1 at 313.933 kb on - strand at 313.942 kb on - strand at 314.001 kb on - strand at 314.020 kb on - strand at 314.334 kb on + strand at 314.334 kb on + strand at 314.334 kb on + strand at 314.336 kb on + strand at 314.451 kb on - strand, within GFF3558 at 314.734 kb on + strand at 314.735 kb on - strand at 314.741 kb on + strand at 314.744 kb on + strand at 314.744 kb on + strand at 314.937 kb on - strand, within GFF3559 at 314.937 kb on - strand, within GFF3559 at 315.012 kb on + strand, within GFF3559 at 315.047 kb on + strand, within GFF3559 at 315.048 kb on - strand, within GFF3559 at 315.073 kb on + strand, within GFF3559 at 315.236 kb on + strand, within GFF3559 at 315.291 kb on + strand, within GFF3559 at 315.368 kb on + strand, within GFF3559 at 315.368 kb on + strand, within GFF3559 at 315.368 kb on + strand, within GFF3559 at 315.368 kb on + strand, within GFF3559 at 315.369 kb on - strand, within GFF3559 at 315.369 kb on - strand, within GFF3559 at 315.373 kb on - strand, within GFF3559 at 315.425 kb on + strand, within GFF3559 at 315.484 kb on - strand, within GFF3559 at 315.484 kb on - strand, within GFF3559 at 315.500 kb on - strand, within GFF3559 at 315.529 kb on - strand, within GFF3559 at 315.529 kb on - strand, within GFF3559 at 315.567 kb on - strand, within GFF3559 at 316.022 kb on + strand, within GFF3559 at 316.022 kb on + strand, within GFF3559 at 316.260 kb on + strand at 316.440 kb on + strand, within GFF3560 at 316.658 kb on + strand, within GFF3560 at 317.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 313,933 - +0.2 313,942 - +1.0 314,001 - +1.1 314,020 - -0.0 314,334 + -0.1 314,334 + +0.8 314,334 + +0.4 314,336 + +0.3 314,451 - GFF3558 0.22 -0.6 314,734 + +0.8 314,735 - -0.6 314,741 + +0.1 314,744 + -0.3 314,744 + -1.1 314,937 - GFF3559 0.13 -0.1 314,937 - GFF3559 0.13 +0.2 315,012 + GFF3559 0.18 +1.1 315,047 + GFF3559 0.20 +0.5 315,048 - GFF3559 0.20 +0.7 315,073 + GFF3559 0.22 -0.7 315,236 + GFF3559 0.33 +0.4 315,291 + GFF3559 0.37 +0.9 315,368 + GFF3559 0.42 +0.2 315,368 + GFF3559 0.42 -1.0 315,368 + GFF3559 0.42 +1.0 315,368 + GFF3559 0.42 +0.0 315,369 - GFF3559 0.42 +0.5 315,369 - GFF3559 0.42 -0.3 315,373 - GFF3559 0.42 +0.3 315,425 + GFF3559 0.46 +0.3 315,484 - GFF3559 0.50 -0.6 315,484 - GFF3559 0.50 -0.9 315,500 - GFF3559 0.51 -2.1 315,529 - GFF3559 0.53 +0.0 315,529 - GFF3559 0.53 +1.7 315,567 - GFF3559 0.55 -0.5 316,022 + GFF3559 0.86 +0.9 316,022 + GFF3559 0.86 +0.6 316,260 + -0.6 316,440 + GFF3560 0.26 +0.2 316,658 + GFF3560 0.55 +0.5 317,067 + +0.6
Or see this region's nucleotide sequence