Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3367 and GFF3368 are separated by 205 nucleotides GFF3368 and GFF3369 are separated by 71 nucleotides GFF3369 and GFF3370 are separated by 197 nucleotides GFF3370 and GFF3371 overlap by 15 nucleotides
GFF3367 - Putative inner membrane lipoprotein, at 135,280 to 136,041
GFF3367
GFF3368 - FIG01046221: hypothetical protein, at 136,247 to 136,471
GFF3368
GFF3369 - FIG146278: Maf/YceF/YhdE family protein, at 136,543 to 137,127
GFF3369
GFF3370 - COG1399 protein, clustered with ribosomal protein L32p, at 137,325 to 137,846
GFF3370
GFF3371 - hypothetical protein, at 137,832 to 138,236
GFF3371
Position (kb) 136
137
138 Strain fitness (log2 ratio) -2
-1
0
1 at 135.568 kb on - strand, within GFF3367 at 135.707 kb on + strand, within GFF3367 at 136.040 kb on - strand at 136.138 kb on + strand at 136.167 kb on - strand at 136.325 kb on - strand, within GFF3368 at 136.438 kb on - strand, within GFF3368 at 136.480 kb on + strand at 136.485 kb on - strand at 136.716 kb on - strand, within GFF3369 at 136.716 kb on - strand, within GFF3369 at 136.716 kb on - strand, within GFF3369 at 136.781 kb on - strand, within GFF3369 at 136.801 kb on - strand, within GFF3369 at 136.802 kb on + strand, within GFF3369 at 136.803 kb on - strand, within GFF3369 at 137.144 kb on - strand at 137.144 kb on - strand at 137.169 kb on - strand at 137.174 kb on - strand at 137.293 kb on - strand at 137.335 kb on + strand at 137.640 kb on + strand, within GFF3370 at 137.656 kb on + strand, within GFF3370 at 137.847 kb on + strand at 138.065 kb on + strand, within GFF3371
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 135,568 - GFF3367 0.38 -0.1 135,707 + GFF3367 0.56 +0.6 136,040 - +0.4 136,138 + -0.1 136,167 - -0.4 136,325 - GFF3368 0.35 +0.9 136,438 - GFF3368 0.85 +0.4 136,480 + -0.6 136,485 - -0.5 136,716 - GFF3369 0.30 -0.8 136,716 - GFF3369 0.30 +1.0 136,716 - GFF3369 0.30 -0.1 136,781 - GFF3369 0.41 +0.7 136,801 - GFF3369 0.44 -0.2 136,802 + GFF3369 0.44 +0.8 136,803 - GFF3369 0.44 +1.1 137,144 - -0.1 137,144 - -0.0 137,169 - +0.6 137,174 - -1.2 137,293 - -2.4 137,335 + +0.7 137,640 + GFF3370 0.60 +0.5 137,656 + GFF3370 0.63 -0.3 137,847 + +1.3 138,065 + GFF3371 0.58 +0.9
Or see this region's nucleotide sequence