Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2656 and GFF2657 are separated by 150 nucleotides GFF2657 and GFF2658 are separated by 54 nucleotides GFF2658 and GFF2659 are separated by 238 nucleotides
GFF2656 - Fumarate and nitrate reduction regulatory protein, at 179,992 to 180,744
GFF2656
GFF2657 - Universal stress protein E, at 180,895 to 181,842
GFF2657
GFF2658 - Putative inner membrane protein, at 181,897 to 182,151
GFF2658
GFF2659 - Mechanosensitive ion channel, at 182,390 to 183,421
GFF2659
Position (kb) 180
181
182 Strain fitness (log2 ratio) -1
0
1 at 179.991 kb on + strand at 180.196 kb on + strand, within GFF2656 at 180.226 kb on + strand, within GFF2656 at 180.258 kb on + strand, within GFF2656 at 180.272 kb on + strand, within GFF2656 at 180.676 kb on - strand at 180.679 kb on + strand at 180.679 kb on + strand at 180.680 kb on - strand at 180.681 kb on + strand at 180.684 kb on + strand at 180.856 kb on + strand at 180.882 kb on - strand at 180.971 kb on - strand at 181.158 kb on + strand, within GFF2657 at 181.158 kb on + strand, within GFF2657 at 181.397 kb on - strand, within GFF2657 at 181.436 kb on + strand, within GFF2657 at 181.461 kb on + strand, within GFF2657 at 181.629 kb on - strand, within GFF2657 at 181.792 kb on - strand at 181.792 kb on - strand at 181.890 kb on - strand at 181.948 kb on - strand, within GFF2658 at 181.995 kb on - strand, within GFF2658 at 181.995 kb on - strand, within GFF2658 at 182.026 kb on - strand, within GFF2658 at 182.026 kb on - strand, within GFF2658 at 182.154 kb on + strand at 182.164 kb on + strand at 182.241 kb on - strand at 182.422 kb on + strand at 182.767 kb on + strand, within GFF2659
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 179,991 + +0.2 180,196 + GFF2656 0.27 +0.7 180,226 + GFF2656 0.31 +0.1 180,258 + GFF2656 0.35 +0.1 180,272 + GFF2656 0.37 -0.9 180,676 - -1.1 180,679 + -0.9 180,679 + -0.8 180,680 - -0.2 180,681 + +0.3 180,684 + +0.1 180,856 + +0.3 180,882 - -1.4 180,971 - -1.2 181,158 + GFF2657 0.28 -1.7 181,158 + GFF2657 0.28 -0.6 181,397 - GFF2657 0.53 +0.4 181,436 + GFF2657 0.57 -0.6 181,461 + GFF2657 0.60 -0.9 181,629 - GFF2657 0.77 -1.0 181,792 - -1.0 181,792 - -1.1 181,890 - +0.2 181,948 - GFF2658 0.20 -0.1 181,995 - GFF2658 0.38 +0.5 181,995 - GFF2658 0.38 -1.6 182,026 - GFF2658 0.51 -0.1 182,026 - GFF2658 0.51 -0.0 182,154 + -0.1 182,164 + -0.1 182,241 - -0.7 182,422 + -0.2 182,767 + GFF2659 0.37 -0.0
Or see this region's nucleotide sequence