Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2553 and GFF2554 are separated by 512 nucleotides GFF2554 and GFF2555 are separated by 94 nucleotides GFF2555 and GFF2556 are separated by 176 nucleotides
GFF2553 - Osmotically inducible protein C, at 69,854 to 70,285
GFF2553
GFF2554 - Bdm protein, at 70,798 to 71,013
GFF2554
GFF2555 - Stationary-phase-induced ribosome-associated protein, at 71,108 to 71,251
GFF2555
GFF2556 - NAD-dependent malic enzyme (EC 1.1.1.38), at 71,428 to 73,125
GFF2556
Position (kb) 71
72 Strain fitness (log2 ratio) -1
0
1 at 70.220 kb on + strand, within GFF2553 at 70.400 kb on - strand at 70.963 kb on + strand, within GFF2554 at 70.963 kb on + strand, within GFF2554 at 70.981 kb on + strand, within GFF2554 at 70.982 kb on - strand, within GFF2554 at 71.047 kb on + strand at 71.079 kb on - strand at 71.136 kb on + strand, within GFF2555 at 71.136 kb on + strand, within GFF2555 at 71.137 kb on - strand, within GFF2555 at 71.179 kb on + strand, within GFF2555 at 71.256 kb on - strand at 71.341 kb on - strand at 71.373 kb on + strand at 71.374 kb on - strand at 71.375 kb on + strand at 71.375 kb on + strand at 71.407 kb on - strand at 71.407 kb on - strand at 71.462 kb on - strand at 71.462 kb on - strand at 71.628 kb on + strand, within GFF2556 at 71.650 kb on - strand, within GFF2556 at 71.650 kb on - strand, within GFF2556 at 71.653 kb on + strand, within GFF2556 at 71.737 kb on + strand, within GFF2556 at 71.961 kb on - strand, within GFF2556 at 71.992 kb on + strand, within GFF2556 at 72.039 kb on - strand, within GFF2556 at 72.094 kb on + strand, within GFF2556 at 72.095 kb on - strand, within GFF2556 at 72.097 kb on + strand, within GFF2556 at 72.228 kb on + strand, within GFF2556
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 70,220 + GFF2553 0.85 +0.0 70,400 - -0.1 70,963 + GFF2554 0.76 -0.1 70,963 + GFF2554 0.76 -0.1 70,981 + GFF2554 0.85 -0.1 70,982 - GFF2554 0.85 +0.6 71,047 + -0.1 71,079 - +1.4 71,136 + GFF2555 0.19 -0.6 71,136 + GFF2555 0.19 -1.5 71,137 - GFF2555 0.20 -1.1 71,179 + GFF2555 0.49 +0.7 71,256 - +0.1 71,341 - +0.4 71,373 + +0.0 71,374 - -0.3 71,375 + +1.5 71,375 + -0.0 71,407 - +0.4 71,407 - -0.4 71,462 - -1.7 71,462 - -0.9 71,628 + GFF2556 0.12 -0.2 71,650 - GFF2556 0.13 -0.4 71,650 - GFF2556 0.13 -0.1 71,653 + GFF2556 0.13 +0.2 71,737 + GFF2556 0.18 -1.5 71,961 - GFF2556 0.31 -0.6 71,992 + GFF2556 0.33 +1.5 72,039 - GFF2556 0.36 -0.3 72,094 + GFF2556 0.39 -0.3 72,095 - GFF2556 0.39 +0.0 72,097 + GFF2556 0.39 +0.1 72,228 + GFF2556 0.47 -1.7
Or see this region's nucleotide sequence