Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2009 and GFF2010 are separated by 43 nucleotides GFF2010 and GFF2011 are separated by 254 nucleotides GFF2011 and GFF2012 are separated by 58 nucleotides GFF2012 and GFF2013 are separated by 340 nucleotides
GFF2009 - Heat shock protein 60 family chaperone GroEL, at 19,717 to 21,363
GFF2009
GFF2010 - Heat shock protein 60 family co-chaperone GroES, at 21,407 to 21,700
GFF2010
GFF2011 - Putative permease, at 21,955 to 23,217
GFF2011
GFF2012 - FxsA protein, at 23,276 to 23,752
GFF2012
GFF2013 - Aspartate ammonia-lyase (EC 4.3.1.1), at 24,093 to 25,529
GFF2013
Position (kb) 21
22
23
24 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 21.386 kb on - strand at 21.735 kb on - strand at 21.754 kb on - strand at 21.864 kb on - strand at 21.874 kb on + strand at 21.898 kb on + strand at 21.898 kb on + strand at 21.899 kb on - strand at 22.344 kb on - strand, within GFF2011 at 22.359 kb on + strand, within GFF2011 at 22.365 kb on - strand, within GFF2011 at 22.371 kb on - strand, within GFF2011 at 22.373 kb on + strand, within GFF2011 at 22.374 kb on - strand, within GFF2011 at 22.436 kb on + strand, within GFF2011 at 22.709 kb on - strand, within GFF2011 at 22.794 kb on + strand, within GFF2011 at 22.831 kb on + strand, within GFF2011 at 22.832 kb on - strand, within GFF2011 at 22.832 kb on - strand, within GFF2011 at 22.832 kb on - strand, within GFF2011 at 22.874 kb on - strand, within GFF2011 at 22.976 kb on - strand, within GFF2011 at 23.011 kb on + strand, within GFF2011 at 23.028 kb on + strand, within GFF2011 at 23.038 kb on + strand, within GFF2011 at 23.049 kb on + strand, within GFF2011 at 23.050 kb on - strand, within GFF2011 at 23.124 kb on - strand at 23.124 kb on - strand at 23.124 kb on - strand at 23.154 kb on - strand at 23.154 kb on - strand at 23.156 kb on - strand at 23.180 kb on + strand at 23.180 kb on + strand at 23.181 kb on - strand at 23.181 kb on - strand at 23.223 kb on - strand at 23.254 kb on + strand at 23.272 kb on - strand at 23.297 kb on - strand at 23.405 kb on + strand, within GFF2012 at 23.608 kb on + strand, within GFF2012 at 23.638 kb on + strand, within GFF2012 at 23.639 kb on - strand, within GFF2012 at 23.699 kb on - strand, within GFF2012 at 23.858 kb on - strand at 23.858 kb on - strand at 23.858 kb on - strand at 23.858 kb on - strand at 23.876 kb on - strand at 23.876 kb on - strand at 23.907 kb on - strand at 23.920 kb on + strand at 24.037 kb on + strand at 24.038 kb on - strand at 24.156 kb on + strand at 24.157 kb on - strand at 24.157 kb on - strand at 24.159 kb on + strand at 24.159 kb on + strand at 24.195 kb on + strand at 24.195 kb on + strand at 24.196 kb on - strand at 24.200 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 21,386 - -1.8 21,735 - -1.9 21,754 - -2.8 21,864 - +0.1 21,874 + -0.5 21,898 + -0.7 21,898 + -0.1 21,899 - +0.4 22,344 - GFF2011 0.31 -0.9 22,359 + GFF2011 0.32 -1.2 22,365 - GFF2011 0.32 +0.1 22,371 - GFF2011 0.33 +0.0 22,373 + GFF2011 0.33 +0.2 22,374 - GFF2011 0.33 +1.1 22,436 + GFF2011 0.38 -0.1 22,709 - GFF2011 0.60 -0.0 22,794 + GFF2011 0.66 -0.1 22,831 + GFF2011 0.69 -1.3 22,832 - GFF2011 0.69 -0.6 22,832 - GFF2011 0.69 -1.1 22,832 - GFF2011 0.69 -0.3 22,874 - GFF2011 0.73 +0.3 22,976 - GFF2011 0.81 +0.9 23,011 + GFF2011 0.84 -0.4 23,028 + GFF2011 0.85 -0.7 23,038 + GFF2011 0.86 -0.9 23,049 + GFF2011 0.87 -0.3 23,050 - GFF2011 0.87 -0.2 23,124 - -0.0 23,124 - -1.9 23,124 - +1.2 23,154 - -0.3 23,154 - +0.2 23,156 - -0.5 23,180 + +0.3 23,180 + -0.1 23,181 - -0.4 23,181 - +3.9 23,223 - +0.6 23,254 + +0.1 23,272 - -0.0 23,297 - -0.2 23,405 + GFF2012 0.27 -0.1 23,608 + GFF2012 0.70 +0.5 23,638 + GFF2012 0.76 -0.4 23,639 - GFF2012 0.76 -0.0 23,699 - GFF2012 0.89 +1.5 23,858 - -0.3 23,858 - +0.4 23,858 - -0.1 23,858 - +0.8 23,876 - -0.1 23,876 - -0.1 23,907 - -0.4 23,920 + +0.2 24,037 + -0.8 24,038 - +0.1 24,156 + -0.5 24,157 - -0.2 24,157 - -0.9 24,159 + -0.0 24,159 + +0.0 24,195 + +0.2 24,195 + -0.6 24,196 - -0.3 24,200 + -0.1
Or see this region's nucleotide sequence