Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1747 and GFF1748 are separated by 324 nucleotides GFF1748 and GFF1749 are separated by 15 nucleotides GFF1749 and GFF1750 are separated by 255 nucleotides
GFF1747 - putative inner membrane protein, at 47,499 to 48,461
GFF1747
GFF1748 - Integrase, at 48,786 to 49,496
GFF1748
GFF1749 - Integrase, at 49,512 to 50,006
GFF1749
GFF1750 - GTPase and tRNA-U34 5-formylation enzyme TrmE, at 50,262 to 51,626
GFF1750
Position (kb)
48
49
50 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 48.319 kb on - strand, within GFF1747 at 48.354 kb on + strand, within GFF1747 at 48.639 kb on + strand at 48.740 kb on - strand at 48.740 kb on - strand at 48.740 kb on - strand at 48.745 kb on - strand at 48.919 kb on + strand, within GFF1748 at 48.920 kb on - strand, within GFF1748 at 48.993 kb on + strand, within GFF1748 at 48.993 kb on + strand, within GFF1748 at 48.993 kb on + strand, within GFF1748 at 48.993 kb on + strand, within GFF1748 at 48.993 kb on + strand, within GFF1748 at 48.994 kb on - strand, within GFF1748 at 49.127 kb on - strand, within GFF1748 at 49.196 kb on + strand, within GFF1748 at 49.196 kb on + strand, within GFF1748 at 49.196 kb on + strand, within GFF1748 at 49.196 kb on + strand, within GFF1748 at 49.197 kb on - strand, within GFF1748 at 49.292 kb on + strand, within GFF1748 at 49.350 kb on - strand, within GFF1748 at 49.357 kb on + strand, within GFF1748 at 49.358 kb on - strand, within GFF1748 at 49.428 kb on - strand at 49.428 kb on - strand at 49.429 kb on + strand at 49.442 kb on + strand at 49.487 kb on - strand at 49.502 kb on + strand at 50.018 kb on - strand at 50.019 kb on + strand at 50.041 kb on - strand at 50.194 kb on + strand at 50.219 kb on - strand at 50.263 kb on + strand at 50.394 kb on + strand at 50.397 kb on + strand at 50.397 kb on + strand at 50.448 kb on + strand, within GFF1750 at 50.449 kb on - strand, within GFF1750
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 48,319 - GFF1747 0.85 +0.1 48,354 + GFF1747 0.89 -0.7 48,639 + +0.2 48,740 - -0.7 48,740 - +0.1 48,740 - -0.1 48,745 - -1.2 48,919 + GFF1748 0.19 +0.1 48,920 - GFF1748 0.19 -0.3 48,993 + GFF1748 0.29 +0.0 48,993 + GFF1748 0.29 -1.9 48,993 + GFF1748 0.29 +0.3 48,993 + GFF1748 0.29 -0.7 48,993 + GFF1748 0.29 -0.5 48,994 - GFF1748 0.29 -0.2 49,127 - GFF1748 0.48 -2.4 49,196 + GFF1748 0.58 +0.2 49,196 + GFF1748 0.58 +0.2 49,196 + GFF1748 0.58 -0.5 49,196 + GFF1748 0.58 -0.1 49,197 - GFF1748 0.58 -0.4 49,292 + GFF1748 0.71 +0.5 49,350 - GFF1748 0.79 +0.1 49,357 + GFF1748 0.80 +0.2 49,358 - GFF1748 0.80 -1.3 49,428 - -0.0 49,428 - +0.0 49,429 + +0.6 49,442 + +0.9 49,487 - -0.1 49,502 + -0.1 50,018 - -0.5 50,019 + -0.2 50,041 - -0.4 50,194 + -1.1 50,219 - +0.6 50,263 + -0.5 50,394 + +1.9 50,397 + -1.1 50,397 + -1.4 50,448 + GFF1750 0.14 -0.8 50,449 - GFF1750 0.14 -1.3
Or see this region's nucleotide sequence