Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1333 and GFF1334 are separated by 30 nucleotides GFF1334 and GFF1335 are separated by 121 nucleotides GFF1335 and GFF1336 are separated by 209 nucleotides GFF1336 and GFF1337 overlap by 14 nucleotides
GFF1333 - Serine transporter, at 41,331 to 42,560
GFF1333
GFF1334 - Uncharacterized protein YohL, at 42,591 to 42,863
GFF1334
GFF1335 - Nickel/cobalt efflux transporter RcnA, at 42,985 to 43,851
GFF1335
GFF1336 - FIG01047778: hypothetical protein, at 44,061 to 44,819
GFF1336
GFF1337 - FIG01045819: hypothetical protein, at 44,806 to 45,318
GFF1337
Position (kb) 42
43
44 Strain fitness (log2 ratio) -1
0
1 at 42.015 kb on - strand, within GFF1333 at 42.109 kb on - strand, within GFF1333 at 42.109 kb on - strand, within GFF1333 at 42.278 kb on - strand, within GFF1333 at 42.368 kb on + strand, within GFF1333 at 42.405 kb on + strand, within GFF1333 at 42.405 kb on + strand, within GFF1333 at 42.406 kb on - strand, within GFF1333 at 42.465 kb on + strand at 42.503 kb on + strand at 42.559 kb on - strand at 42.717 kb on + strand, within GFF1334 at 43.093 kb on - strand, within GFF1335 at 43.093 kb on - strand, within GFF1335 at 43.107 kb on + strand, within GFF1335 at 43.108 kb on - strand, within GFF1335 at 43.210 kb on + strand, within GFF1335 at 43.210 kb on + strand, within GFF1335 at 43.442 kb on + strand, within GFF1335 at 43.442 kb on + strand, within GFF1335 at 43.442 kb on + strand, within GFF1335 at 43.442 kb on + strand, within GFF1335 at 43.443 kb on - strand, within GFF1335 at 43.443 kb on - strand, within GFF1335 at 43.443 kb on - strand, within GFF1335 at 43.471 kb on + strand, within GFF1335 at 43.539 kb on + strand, within GFF1335 at 43.635 kb on + strand, within GFF1335 at 43.813 kb on + strand at 43.813 kb on + strand at 43.816 kb on - strand at 43.832 kb on - strand at 43.854 kb on - strand at 44.062 kb on + strand at 44.319 kb on - strand, within GFF1336 at 44.321 kb on - strand, within GFF1336 at 44.321 kb on - strand, within GFF1336 at 44.472 kb on + strand, within GFF1336 at 44.473 kb on - strand, within GFF1336 at 44.473 kb on - strand, within GFF1336 at 44.603 kb on - strand, within GFF1336 at 44.641 kb on - strand, within GFF1336 at 44.696 kb on - strand, within GFF1336
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 42,015 - GFF1333 0.56 -0.1 42,109 - GFF1333 0.63 -1.6 42,109 - GFF1333 0.63 -0.8 42,278 - GFF1333 0.77 +0.1 42,368 + GFF1333 0.84 -0.4 42,405 + GFF1333 0.87 -0.2 42,405 + GFF1333 0.87 +0.5 42,406 - GFF1333 0.87 -0.3 42,465 + +0.4 42,503 + -0.4 42,559 - -0.3 42,717 + GFF1334 0.46 -1.1 43,093 - GFF1335 0.12 -0.0 43,093 - GFF1335 0.12 -0.3 43,107 + GFF1335 0.14 -0.0 43,108 - GFF1335 0.14 +0.1 43,210 + GFF1335 0.26 +0.5 43,210 + GFF1335 0.26 -0.1 43,442 + GFF1335 0.53 +0.5 43,442 + GFF1335 0.53 +0.4 43,442 + GFF1335 0.53 -0.0 43,442 + GFF1335 0.53 -0.2 43,443 - GFF1335 0.53 +0.3 43,443 - GFF1335 0.53 -1.0 43,443 - GFF1335 0.53 -0.7 43,471 + GFF1335 0.56 -0.6 43,539 + GFF1335 0.64 -0.8 43,635 + GFF1335 0.75 +0.1 43,813 + -0.2 43,813 + +0.0 43,816 - -0.1 43,832 - -0.5 43,854 - -0.0 44,062 + -0.2 44,319 - GFF1336 0.34 -0.1 44,321 - GFF1336 0.34 +0.6 44,321 - GFF1336 0.34 +0.6 44,472 + GFF1336 0.54 -0.8 44,473 - GFF1336 0.54 -1.3 44,473 - GFF1336 0.54 +0.3 44,603 - GFF1336 0.71 +0.4 44,641 - GFF1336 0.76 +0.3 44,696 - GFF1336 0.84 +0.1
Or see this region's nucleotide sequence