Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1271 and GFF1272 are separated by 482 nucleotides GFF1272 and GFF1273 overlap by 29 nucleotides
GFF1271 - GtgA, at 58 to 531
GFF1271
GFF1272 - Phage holin, at 1,014 to 1,349
GFF1272
GFF1273 - Phage lysin (EC 3.2.1.17) # Phage lysozyme or muramidase (EC 3.2.1.17), at 1,321 to 1,785
GFF1273
Position (kb) 0
1 Strain fitness (log2 ratio) -2
-1
0
1 at 0.024 kb on + strand at 0.127 kb on - strand, within GFF1271 at 0.127 kb on - strand, within GFF1271 at 0.193 kb on + strand, within GFF1271 at 0.194 kb on - strand, within GFF1271 at 0.194 kb on - strand, within GFF1271 at 0.244 kb on - strand, within GFF1271 at 0.263 kb on - strand, within GFF1271 at 0.340 kb on + strand, within GFF1271 at 0.340 kb on + strand, within GFF1271 at 0.341 kb on - strand, within GFF1271 at 0.349 kb on - strand, within GFF1271 at 0.388 kb on + strand, within GFF1271 at 0.388 kb on + strand, within GFF1271 at 0.452 kb on + strand, within GFF1271 at 0.452 kb on + strand, within GFF1271 at 0.453 kb on - strand, within GFF1271 at 0.479 kb on - strand, within GFF1271 at 0.497 kb on + strand at 0.498 kb on - strand at 0.501 kb on + strand at 0.501 kb on + strand at 0.549 kb on + strand at 0.554 kb on + strand at 0.879 kb on + strand at 0.945 kb on + strand at 0.981 kb on - strand at 1.013 kb on + strand at 1.132 kb on + strand, within GFF1272 at 1.133 kb on - strand, within GFF1272 at 1.134 kb on + strand, within GFF1272 at 1.134 kb on + strand, within GFF1272 at 1.134 kb on + strand, within GFF1272 at 1.195 kb on + strand, within GFF1272 at 1.196 kb on - strand, within GFF1272 at 1.304 kb on + strand, within GFF1272 at 1.476 kb on + strand, within GFF1273
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 24 + -0.6 127 - GFF1271 0.15 -0.8 127 - GFF1271 0.15 +0.1 193 + GFF1271 0.28 +0.3 194 - GFF1271 0.29 -0.1 194 - GFF1271 0.29 +0.1 244 - GFF1271 0.39 -2.3 263 - GFF1271 0.43 +0.7 340 + GFF1271 0.59 -1.3 340 + GFF1271 0.59 -1.0 341 - GFF1271 0.60 -0.5 349 - GFF1271 0.61 -0.5 388 + GFF1271 0.70 -0.4 388 + GFF1271 0.70 -0.3 452 + GFF1271 0.83 -0.7 452 + GFF1271 0.83 +0.3 453 - GFF1271 0.83 -1.4 479 - GFF1271 0.89 -0.5 497 + +0.4 498 - +0.2 501 + -1.0 501 + -0.2 549 + -0.2 554 + -0.2 879 + +0.7 945 + -0.3 981 - +0.7 1,013 + -0.4 1,132 + GFF1272 0.35 +0.8 1,133 - GFF1272 0.35 +0.4 1,134 + GFF1272 0.36 +1.0 1,134 + GFF1272 0.36 -0.4 1,134 + GFF1272 0.36 -0.3 1,195 + GFF1272 0.54 +0.3 1,196 - GFF1272 0.54 -0.2 1,304 + GFF1272 0.86 +0.3 1,476 + GFF1273 0.33 -0.6
Or see this region's nucleotide sequence