Experiment: L-Pipecolic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3029 and Atu3030 are separated by 32 nucleotides Atu3030 and Atu3031 are separated by 83 nucleotides Atu3031 and Atu3032 are separated by 44 nucleotides Atu3032 and Atu3033 are separated by 64 nucleotides Atu3033 and Atu3034 are separated by 18 nucleotides
Atu3029: Atu3029 - glucarate dehydratase, at 24,114 to 25,361
Atu3029
Atu3030: Atu3030 - hypothetical protein, at 25,394 to 25,780
Atu3030
Atu3031: Atu3031 - hypothetical protein, at 25,864 to 26,082
Atu3031
Atu3032: Atu3032 - hypothetical protein, at 26,127 to 26,531
Atu3032
Atu3033: Atu3033 - hypothetical protein, at 26,596 to 26,835
Atu3033
Atu3034: Atu3034 - ROK family transcriptional regulator, at 26,854 to 27,402
Atu3034
Position (kb)
26
27 Strain fitness (log2 ratio)
-1
0
1
2 at 25.224 kb on - strand, within Atu3029 at 25.244 kb on + strand at 25.278 kb on - strand at 25.293 kb on - strand at 25.376 kb on + strand at 25.490 kb on + strand, within Atu3030 at 25.498 kb on - strand, within Atu3030 at 25.498 kb on - strand, within Atu3030 at 25.498 kb on - strand, within Atu3030 at 25.508 kb on + strand, within Atu3030 at 25.665 kb on - strand, within Atu3030 at 25.855 kb on - strand at 25.947 kb on + strand, within Atu3031 at 26.094 kb on + strand at 26.111 kb on + strand at 26.134 kb on + strand at 26.341 kb on + strand, within Atu3032 at 26.362 kb on + strand, within Atu3032 at 26.436 kb on + strand, within Atu3032 at 26.592 kb on - strand at 26.599 kb on - strand at 26.599 kb on - strand at 26.599 kb on - strand at 26.599 kb on - strand at 26.602 kb on + strand at 26.604 kb on - strand at 26.640 kb on + strand, within Atu3033 at 26.681 kb on + strand, within Atu3033 at 26.681 kb on + strand, within Atu3033 at 26.699 kb on - strand, within Atu3033 at 26.706 kb on + strand, within Atu3033 at 26.706 kb on + strand, within Atu3033 at 26.706 kb on + strand, within Atu3033 at 26.706 kb on + strand, within Atu3033 at 26.714 kb on - strand, within Atu3033 at 26.714 kb on - strand, within Atu3033 at 26.714 kb on - strand, within Atu3033 at 26.829 kb on + strand at 26.829 kb on + strand at 26.837 kb on - strand at 26.879 kb on - strand at 26.888 kb on - strand at 27.004 kb on - strand, within Atu3034 at 27.015 kb on + strand, within Atu3034 at 27.186 kb on + strand, within Atu3034 at 27.228 kb on + strand, within Atu3034 at 27.236 kb on - strand, within Atu3034 at 27.399 kb on - strand at 27.399 kb on - strand at 27.410 kb on - strand at 27.508 kb on - strand at 27.519 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Pipecolic Acid (N) remove 25,224 - Atu3029 0.89 +0.2 25,244 + +1.1 25,278 - +0.9 25,293 - -0.8 25,376 + +0.0 25,490 + Atu3030 0.25 +0.4 25,498 - Atu3030 0.27 -0.3 25,498 - Atu3030 0.27 -0.0 25,498 - Atu3030 0.27 +1.8 25,508 + Atu3030 0.29 -0.2 25,665 - Atu3030 0.70 -0.0 25,855 - -0.1 25,947 + Atu3031 0.38 -0.4 26,094 + +0.4 26,111 + -0.1 26,134 + +0.5 26,341 + Atu3032 0.53 -0.5 26,362 + Atu3032 0.58 -0.5 26,436 + Atu3032 0.76 -0.7 26,592 - +0.4 26,599 - -0.7 26,599 - +0.1 26,599 - -0.4 26,599 - -0.7 26,602 + -0.5 26,604 - -1.1 26,640 + Atu3033 0.18 +0.1 26,681 + Atu3033 0.35 +0.5 26,681 + Atu3033 0.35 -0.1 26,699 - Atu3033 0.43 -0.2 26,706 + Atu3033 0.46 +0.2 26,706 + Atu3033 0.46 -0.3 26,706 + Atu3033 0.46 -0.4 26,706 + Atu3033 0.46 +1.1 26,714 - Atu3033 0.49 -0.4 26,714 - Atu3033 0.49 +0.1 26,714 - Atu3033 0.49 -0.0 26,829 + +0.5 26,829 + -0.6 26,837 - +0.0 26,879 - +0.9 26,888 - +0.7 27,004 - Atu3034 0.27 -0.4 27,015 + Atu3034 0.29 +0.1 27,186 + Atu3034 0.60 -0.9 27,228 + Atu3034 0.68 -1.1 27,236 - Atu3034 0.70 -0.1 27,399 - +1.2 27,399 - -0.2 27,410 - +0.2 27,508 - -1.0 27,519 - +0.0
Or see this region's nucleotide sequence