Experiment: L-Pipecolic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0535 and Atu0536 are separated by 2 nucleotides Atu0536 and Atu0537 are separated by 3 nucleotides Atu0537 and Atu0538 are separated by 133 nucleotides Atu0538 and Atu0539 are separated by 113 nucleotides
Atu0535: Atu0535 - hypothetical protein, at 526,902 to 527,390
Atu0535
Atu0536: Atu0536 - hypothetical protein, at 527,393 to 527,866
Atu0536
Atu0537: Atu0537 - histidinol dehydrogenase, at 527,870 to 529,168
Atu0537
Atu0538: Atu0538 - hypothetical protein, at 529,302 to 529,742
Atu0538
Atu0539: Atu0539 - UDP-N-acetylglucosamine, at 529,856 to 531,148
Atu0539
Position (kb) 527
528
529
530 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 527.068 kb on - strand, within Atu0535 at 527.103 kb on + strand, within Atu0535 at 527.153 kb on + strand, within Atu0535 at 527.153 kb on + strand, within Atu0535 at 527.161 kb on - strand, within Atu0535 at 527.161 kb on - strand, within Atu0535 at 527.161 kb on - strand, within Atu0535 at 527.161 kb on - strand, within Atu0535 at 527.260 kb on - strand, within Atu0535 at 527.390 kb on + strand at 527.390 kb on + strand at 527.455 kb on + strand, within Atu0536 at 527.550 kb on - strand, within Atu0536 at 527.649 kb on + strand, within Atu0536 at 527.657 kb on - strand, within Atu0536 at 527.657 kb on - strand, within Atu0536 at 527.678 kb on + strand, within Atu0536 at 527.802 kb on + strand, within Atu0536 at 527.802 kb on + strand, within Atu0536 at 527.802 kb on + strand, within Atu0536 at 527.810 kb on - strand, within Atu0536 at 527.876 kb on - strand at 527.922 kb on - strand at 527.930 kb on - strand at 527.940 kb on - strand at 527.979 kb on - strand at 527.979 kb on - strand at 528.038 kb on - strand, within Atu0537 at 528.105 kb on - strand, within Atu0537 at 528.143 kb on - strand, within Atu0537 at 528.348 kb on - strand, within Atu0537 at 528.424 kb on - strand, within Atu0537 at 528.489 kb on + strand, within Atu0537 at 528.497 kb on - strand, within Atu0537 at 528.525 kb on + strand, within Atu0537 at 528.660 kb on + strand, within Atu0537 at 528.683 kb on - strand, within Atu0537 at 528.737 kb on + strand, within Atu0537 at 528.737 kb on + strand, within Atu0537 at 528.745 kb on - strand, within Atu0537 at 528.828 kb on - strand, within Atu0537 at 528.868 kb on + strand, within Atu0537 at 529.035 kb on - strand, within Atu0537 at 529.048 kb on - strand at 529.058 kb on - strand at 529.058 kb on - strand at 529.069 kb on + strand at 529.178 kb on - strand at 529.241 kb on + strand at 529.356 kb on - strand, within Atu0538 at 529.378 kb on - strand, within Atu0538 at 529.482 kb on + strand, within Atu0538 at 529.662 kb on - strand, within Atu0538 at 529.685 kb on + strand, within Atu0538 at 529.777 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Pipecolic Acid (N) remove 527,068 - Atu0535 0.34 +0.5 527,103 + Atu0535 0.41 +0.8 527,153 + Atu0535 0.51 +1.5 527,153 + Atu0535 0.51 +1.2 527,161 - Atu0535 0.53 +0.8 527,161 - Atu0535 0.53 +0.9 527,161 - Atu0535 0.53 +1.1 527,161 - Atu0535 0.53 +0.9 527,260 - Atu0535 0.73 +1.7 527,390 + +1.0 527,390 + -0.2 527,455 + Atu0536 0.13 -1.2 527,550 - Atu0536 0.33 -1.0 527,649 + Atu0536 0.54 -0.8 527,657 - Atu0536 0.56 -0.7 527,657 - Atu0536 0.56 -0.7 527,678 + Atu0536 0.60 -0.9 527,802 + Atu0536 0.86 -0.5 527,802 + Atu0536 0.86 -1.8 527,802 + Atu0536 0.86 -0.6 527,810 - Atu0536 0.88 -0.6 527,876 - +0.1 527,922 - -0.6 527,930 - -5.4 527,940 - -3.5 527,979 - -4.2 527,979 - -3.8 528,038 - Atu0537 0.13 -1.5 528,105 - Atu0537 0.18 -2.3 528,143 - Atu0537 0.21 -2.5 528,348 - Atu0537 0.37 -1.8 528,424 - Atu0537 0.43 -5.8 528,489 + Atu0537 0.48 -2.0 528,497 - Atu0537 0.48 -1.3 528,525 + Atu0537 0.50 -1.9 528,660 + Atu0537 0.61 -2.3 528,683 - Atu0537 0.63 -3.3 528,737 + Atu0537 0.67 +0.7 528,737 + Atu0537 0.67 -4.5 528,745 - Atu0537 0.67 -3.8 528,828 - Atu0537 0.74 +0.7 528,868 + Atu0537 0.77 -2.8 529,035 - Atu0537 0.90 -2.6 529,048 - -5.1 529,058 - -2.8 529,058 - -1.5 529,069 + -1.1 529,178 - +0.1 529,241 + -1.2 529,356 - Atu0538 0.12 +0.1 529,378 - Atu0538 0.17 -0.6 529,482 + Atu0538 0.41 -1.3 529,662 - Atu0538 0.82 +0.0 529,685 + Atu0538 0.87 +1.3 529,777 + +0.1
Or see this region's nucleotide sequence