Experiment: L-Pipecolic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0039 and Atu0040 overlap by 4 nucleotides Atu0040 and Atu0041 are separated by 1 nucleotides Atu0041 and Atu8115 are separated by 1 nucleotides Atu8115 and Atu0043 are separated by 22 nucleotides
Atu0039: Atu0039 - imidazoleglycerol-phosphate synthase cyclase, at 41,118 to 41,894
Atu0039
Atu0040: Atu0040 - phosphoribosylformino-5-aminoimidazole carboxamide ribotide isomerase, at 41,891 to 42,634
Atu0040
Atu0041: Atu0041 - glutamine amidotransferase, at 42,636 to 43,286
Atu0041
Atu8115: Atu8115 - Hypothetical Protein, at 43,288 to 43,782
Atu8115
Atu0043: Atu0043 - imidazoleglycerol-phosphate dehydratase, at 43,805 to 44,401
Atu0043
Position (kb) 42
43
44 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 41.671 kb on + strand, within Atu0039 at 41.834 kb on - strand at 41.905 kb on + strand at 41.913 kb on + strand at 42.070 kb on - strand, within Atu0040 at 42.309 kb on - strand, within Atu0040 at 42.649 kb on - strand at 42.668 kb on + strand at 42.740 kb on - strand, within Atu0041 at 42.958 kb on + strand, within Atu0041 at 42.990 kb on - strand, within Atu0041 at 43.103 kb on - strand, within Atu0041 at 43.381 kb on - strand, within Atu8115 at 43.393 kb on + strand, within Atu8115 at 43.395 kb on + strand, within Atu8115 at 43.431 kb on + strand, within Atu8115 at 43.432 kb on + strand, within Atu8115 at 43.617 kb on - strand, within Atu8115 at 43.668 kb on + strand, within Atu8115 at 43.668 kb on + strand, within Atu8115 at 43.668 kb on + strand, within Atu8115 at 43.668 kb on + strand, within Atu8115 at 43.668 kb on + strand, within Atu8115 at 43.668 kb on + strand, within Atu8115 at 43.675 kb on - strand, within Atu8115 at 43.676 kb on - strand, within Atu8115 at 43.676 kb on - strand, within Atu8115 at 43.676 kb on - strand, within Atu8115 at 43.676 kb on - strand, within Atu8115 at 43.676 kb on - strand, within Atu8115 at 43.694 kb on + strand, within Atu8115 at 43.796 kb on + strand at 43.817 kb on + strand at 43.942 kb on + strand, within Atu0043 at 44.052 kb on + strand, within Atu0043 at 44.128 kb on + strand, within Atu0043 at 44.128 kb on + strand, within Atu0043 at 44.286 kb on - strand, within Atu0043
Per-strain Table
Position Strand Gene LocusTag Fraction L-Pipecolic Acid (N) remove 41,671 + Atu0039 0.71 -1.1 41,834 - -0.1 41,905 + +0.1 41,913 + -1.8 42,070 - Atu0040 0.24 -1.3 42,309 - Atu0040 0.56 -2.4 42,649 - -1.7 42,668 + -0.9 42,740 - Atu0041 0.16 -2.6 42,958 + Atu0041 0.49 -1.9 42,990 - Atu0041 0.54 -1.3 43,103 - Atu0041 0.72 -1.1 43,381 - Atu8115 0.19 -0.7 43,393 + Atu8115 0.21 -2.5 43,395 + Atu8115 0.22 +0.3 43,431 + Atu8115 0.29 +0.7 43,432 + Atu8115 0.29 +1.0 43,617 - Atu8115 0.66 +0.0 43,668 + Atu8115 0.77 -1.1 43,668 + Atu8115 0.77 -0.5 43,668 + Atu8115 0.77 -0.3 43,668 + Atu8115 0.77 -0.9 43,668 + Atu8115 0.77 +0.2 43,668 + Atu8115 0.77 -0.5 43,675 - Atu8115 0.78 -1.2 43,676 - Atu8115 0.78 -1.3 43,676 - Atu8115 0.78 -1.4 43,676 - Atu8115 0.78 -1.1 43,676 - Atu8115 0.78 -1.0 43,676 - Atu8115 0.78 -1.8 43,694 + Atu8115 0.82 -0.6 43,796 + -0.1 43,817 + -3.7 43,942 + Atu0043 0.23 -3.1 44,052 + Atu0043 0.41 -1.8 44,128 + Atu0043 0.54 -0.5 44,128 + Atu0043 0.54 -3.7 44,286 - Atu0043 0.81 -0.1
Or see this region's nucleotide sequence