Experiment: L-Pipecolic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0537 and Atu0538 are separated by 133 nucleotides Atu0538 and Atu0539 are separated by 113 nucleotides Atu0539 and Atu0540 are separated by 102 nucleotides Atu0540 and Atu0541 are separated by 155 nucleotides Atu0541 and Atu0542 are separated by 188 nucleotides
Atu0537: Atu0537 - histidinol dehydrogenase, at 527,870 to 529,168
Atu0537
Atu0538: Atu0538 - hypothetical protein, at 529,302 to 529,742
Atu0538
Atu0539: Atu0539 - UDP-N-acetylglucosamine, at 529,856 to 531,148
Atu0539
Atu0540: Atu0540 - hypothetical protein, at 531,251 to 531,517
Atu0540
Atu0541: Atu0541 - tRNA-Thr, at 531,673 to 531,747
Atu0541
Atu0542: Atu0542 - flagellin, at 531,936 to 532,877
Atu0542
Position (kb) 529
530
531
532 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 528.868 kb on + strand, within Atu0537 at 529.035 kb on - strand, within Atu0537 at 529.048 kb on - strand at 529.058 kb on - strand at 529.058 kb on - strand at 529.069 kb on + strand at 529.178 kb on - strand at 529.241 kb on + strand at 529.356 kb on - strand, within Atu0538 at 529.378 kb on - strand, within Atu0538 at 529.482 kb on + strand, within Atu0538 at 529.662 kb on - strand, within Atu0538 at 529.685 kb on + strand, within Atu0538 at 529.777 kb on + strand at 531.309 kb on - strand, within Atu0540 at 531.334 kb on - strand, within Atu0540 at 531.425 kb on - strand, within Atu0540 at 531.496 kb on + strand at 531.500 kb on + strand at 531.830 kb on - strand at 531.895 kb on + strand at 531.904 kb on + strand at 532.024 kb on + strand at 532.024 kb on + strand at 532.032 kb on - strand, within Atu0542 at 532.032 kb on - strand, within Atu0542 at 532.032 kb on - strand, within Atu0542 at 532.052 kb on + strand, within Atu0542 at 532.071 kb on + strand, within Atu0542
Per-strain Table
Position Strand Gene LocusTag Fraction L-Pipecolic Acid (N) remove 528,868 + Atu0537 0.77 -1.8 529,035 - Atu0537 0.90 -2.6 529,048 - -4.7 529,058 - -3.8 529,058 - -1.5 529,069 + -1.1 529,178 - +0.3 529,241 + -0.2 529,356 - Atu0538 0.12 +0.9 529,378 - Atu0538 0.17 -0.6 529,482 + Atu0538 0.41 +0.0 529,662 - Atu0538 0.82 +0.0 529,685 + Atu0538 0.87 +1.0 529,777 + +0.0 531,309 - Atu0540 0.22 -0.2 531,334 - Atu0540 0.31 +0.5 531,425 - Atu0540 0.65 +1.0 531,496 + +0.3 531,500 + +0.5 531,830 - -0.6 531,895 + +1.5 531,904 + +0.2 532,024 + +0.7 532,024 + -0.6 532,032 - Atu0542 0.10 -0.7 532,032 - Atu0542 0.10 -0.2 532,032 - Atu0542 0.10 +0.8 532,052 + Atu0542 0.12 +0.2 532,071 + Atu0542 0.14 -0.6
Or see this region's nucleotide sequence