Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF963

Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF961 and GFF962 overlap by 4 nucleotidesGFF962 and GFF963 overlap by 14 nucleotidesGFF963 and GFF964 overlap by 1 nucleotides GFF961 - putative cytoplasmic protein, at 142,124 to 144,727 GFF961 GFF962 - putative cytoplasmic protein, at 144,724 to 145,536 GFF962 GFF963 - putative ABC-type sugar/spermidine/putrescine transport system ATPase component, at 145,523 to 146,614 GFF963 GFF964 - putative type II restriction enzyme methylase subunit, at 146,614 to 150,291 GFF964 Position (kb) 145 146 147Strain fitness (log2 ratio) -2 -1 0 1 2at 144.867 kb on + strand, within GFF962at 144.919 kb on - strand, within GFF962at 144.934 kb on + strand, within GFF962at 144.935 kb on - strand, within GFF962at 145.041 kb on + strand, within GFF962at 145.042 kb on - strand, within GFF962at 145.150 kb on + strand, within GFF962at 145.252 kb on + strand, within GFF962at 145.503 kb on + strandat 145.587 kb on + strandat 145.722 kb on - strand, within GFF963at 145.722 kb on - strand, within GFF963at 145.740 kb on - strand, within GFF963at 145.802 kb on + strand, within GFF963at 145.802 kb on + strand, within GFF963at 145.978 kb on + strand, within GFF963at 145.992 kb on + strand, within GFF963at 145.993 kb on - strand, within GFF963at 145.993 kb on - strand, within GFF963at 145.993 kb on - strand, within GFF963at 146.096 kb on - strand, within GFF963at 146.123 kb on + strand, within GFF963at 146.124 kb on - strand, within GFF963at 146.124 kb on - strand, within GFF963at 146.144 kb on - strand, within GFF963at 146.168 kb on - strand, within GFF963at 146.169 kb on + strand, within GFF963at 146.170 kb on - strand, within GFF963at 146.170 kb on - strand, within GFF963at 146.198 kb on - strand, within GFF963at 146.198 kb on - strand, within GFF963at 146.313 kb on - strand, within GFF963at 146.395 kb on - strand, within GFF963at 146.410 kb on + strand, within GFF963at 146.619 kb on + strandat 146.619 kb on + strandat 146.620 kb on - strandat 146.685 kb on + strandat 146.713 kb on - strandat 146.733 kb on - strandat 146.733 kb on - strandat 146.890 kb on + strandat 146.890 kb on + strandat 146.895 kb on - strandat 146.931 kb on - strandat 146.990 kb on + strand, within GFF964at 147.260 kb on + strand, within GFF964at 147.261 kb on - strand, within GFF964at 147.312 kb on - strand, within GFF964at 147.312 kb on - strand, within GFF964at 147.365 kb on + strand, within GFF964at 147.380 kb on - strand, within GFF964

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI
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144,867 + GFF962 0.18 +0.1
144,919 - GFF962 0.24 +0.1
144,934 + GFF962 0.26 +0.1
144,935 - GFF962 0.26 +0.1
145,041 + GFF962 0.39 +2.3
145,042 - GFF962 0.39 +0.4
145,150 + GFF962 0.52 +0.2
145,252 + GFF962 0.65 +0.4
145,503 + -0.4
145,587 + -0.1
145,722 - GFF963 0.18 +1.1
145,722 - GFF963 0.18 +0.9
145,740 - GFF963 0.20 -2.6
145,802 + GFF963 0.26 -0.6
145,802 + GFF963 0.26 -0.3
145,978 + GFF963 0.42 +0.5
145,992 + GFF963 0.43 -0.3
145,993 - GFF963 0.43 +1.1
145,993 - GFF963 0.43 -0.2
145,993 - GFF963 0.43 +0.3
146,096 - GFF963 0.52 +0.1
146,123 + GFF963 0.55 +1.0
146,124 - GFF963 0.55 -0.2
146,124 - GFF963 0.55 -0.1
146,144 - GFF963 0.57 +0.2
146,168 - GFF963 0.59 +0.0
146,169 + GFF963 0.59 -0.5
146,170 - GFF963 0.59 +1.2
146,170 - GFF963 0.59 +0.1
146,198 - GFF963 0.62 +0.7
146,198 - GFF963 0.62 -2.0
146,313 - GFF963 0.72 -0.2
146,395 - GFF963 0.80 +0.1
146,410 + GFF963 0.81 +0.3
146,619 + +0.2
146,619 + +2.3
146,620 - +0.1
146,685 + -0.0
146,713 - +0.2
146,733 - -0.5
146,733 - +0.2
146,890 + +0.3
146,890 + +0.0
146,895 - -0.2
146,931 - +0.2
146,990 + GFF964 0.10 -0.3
147,260 + GFF964 0.18 +0.3
147,261 - GFF964 0.18 +0.1
147,312 - GFF964 0.19 +0.0
147,312 - GFF964 0.19 -0.5
147,365 + GFF964 0.20 -1.0
147,380 - GFF964 0.21 -0.2

Or see this region's nucleotide sequence