Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF961 and GFF962 overlap by 4 nucleotides GFF962 and GFF963 overlap by 14 nucleotides GFF963 and GFF964 overlap by 1 nucleotides
GFF961 - putative cytoplasmic protein, at 142,124 to 144,727
GFF961
GFF962 - putative cytoplasmic protein, at 144,724 to 145,536
GFF962
GFF963 - putative ABC-type sugar/spermidine/putrescine transport system ATPase component, at 145,523 to 146,614
GFF963
GFF964 - putative type II restriction enzyme methylase subunit, at 146,614 to 150,291
GFF964
Position (kb)
145
146
147 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 144.867 kb on + strand, within GFF962 at 144.919 kb on - strand, within GFF962 at 144.934 kb on + strand, within GFF962 at 144.935 kb on - strand, within GFF962 at 145.041 kb on + strand, within GFF962 at 145.042 kb on - strand, within GFF962 at 145.150 kb on + strand, within GFF962 at 145.252 kb on + strand, within GFF962 at 145.503 kb on + strand at 145.587 kb on + strand at 145.722 kb on - strand, within GFF963 at 145.722 kb on - strand, within GFF963 at 145.740 kb on - strand, within GFF963 at 145.802 kb on + strand, within GFF963 at 145.802 kb on + strand, within GFF963 at 145.978 kb on + strand, within GFF963 at 145.992 kb on + strand, within GFF963 at 145.993 kb on - strand, within GFF963 at 145.993 kb on - strand, within GFF963 at 145.993 kb on - strand, within GFF963 at 146.096 kb on - strand, within GFF963 at 146.123 kb on + strand, within GFF963 at 146.124 kb on - strand, within GFF963 at 146.124 kb on - strand, within GFF963 at 146.144 kb on - strand, within GFF963 at 146.168 kb on - strand, within GFF963 at 146.169 kb on + strand, within GFF963 at 146.170 kb on - strand, within GFF963 at 146.170 kb on - strand, within GFF963 at 146.198 kb on - strand, within GFF963 at 146.198 kb on - strand, within GFF963 at 146.313 kb on - strand, within GFF963 at 146.395 kb on - strand, within GFF963 at 146.410 kb on + strand, within GFF963 at 146.619 kb on + strand at 146.619 kb on + strand at 146.620 kb on - strand at 146.685 kb on + strand at 146.713 kb on - strand at 146.733 kb on - strand at 146.733 kb on - strand at 146.890 kb on + strand at 146.890 kb on + strand at 146.895 kb on - strand at 146.931 kb on - strand at 146.990 kb on + strand, within GFF964 at 147.260 kb on + strand, within GFF964 at 147.261 kb on - strand, within GFF964 at 147.312 kb on - strand, within GFF964 at 147.312 kb on - strand, within GFF964 at 147.365 kb on + strand, within GFF964 at 147.380 kb on - strand, within GFF964
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 144,867 + GFF962 0.18 +0.1 144,919 - GFF962 0.24 +0.1 144,934 + GFF962 0.26 +0.1 144,935 - GFF962 0.26 +0.1 145,041 + GFF962 0.39 +2.3 145,042 - GFF962 0.39 +0.4 145,150 + GFF962 0.52 +0.2 145,252 + GFF962 0.65 +0.4 145,503 + -0.4 145,587 + -0.1 145,722 - GFF963 0.18 +1.1 145,722 - GFF963 0.18 +0.9 145,740 - GFF963 0.20 -2.6 145,802 + GFF963 0.26 -0.6 145,802 + GFF963 0.26 -0.3 145,978 + GFF963 0.42 +0.5 145,992 + GFF963 0.43 -0.3 145,993 - GFF963 0.43 +1.1 145,993 - GFF963 0.43 -0.2 145,993 - GFF963 0.43 +0.3 146,096 - GFF963 0.52 +0.1 146,123 + GFF963 0.55 +1.0 146,124 - GFF963 0.55 -0.2 146,124 - GFF963 0.55 -0.1 146,144 - GFF963 0.57 +0.2 146,168 - GFF963 0.59 +0.0 146,169 + GFF963 0.59 -0.5 146,170 - GFF963 0.59 +1.2 146,170 - GFF963 0.59 +0.1 146,198 - GFF963 0.62 +0.7 146,198 - GFF963 0.62 -2.0 146,313 - GFF963 0.72 -0.2 146,395 - GFF963 0.80 +0.1 146,410 + GFF963 0.81 +0.3 146,619 + +0.2 146,619 + +2.3 146,620 - +0.1 146,685 + -0.0 146,713 - +0.2 146,733 - -0.5 146,733 - +0.2 146,890 + +0.3 146,890 + +0.0 146,895 - -0.2 146,931 - +0.2 146,990 + GFF964 0.10 -0.3 147,260 + GFF964 0.18 +0.3 147,261 - GFF964 0.18 +0.1 147,312 - GFF964 0.19 +0.0 147,312 - GFF964 0.19 -0.5 147,365 + GFF964 0.20 -1.0 147,380 - GFF964 0.21 -0.2
Or see this region's nucleotide sequence