Experiment: LB_plus_SM_buffer with phi19h1_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF930 and GFF931 are separated by 226 nucleotides GFF931 and GFF932 overlap by 4 nucleotides GFF932 and GFF933 are separated by 73 nucleotides
GFF930 - Aspartate carbamoyltransferase (EC 2.1.3.2), at 107,322 to 108,257
GFF930
GFF931 - Arginine pathway regulatory protein ArgR, repressor of arg regulon, at 108,484 to 108,972
GFF931
GFF932 - hypothetical protein, at 108,969 to 109,085
GFF932
GFF933 - Arginine/ornithine antiporter ArcD, at 109,159 to 110,562
GFF933
Position (kb)
108
109 Strain fitness (log2 ratio)
-1
0
1 at 107.565 kb on + strand, within GFF930 at 107.577 kb on - strand, within GFF930 at 107.577 kb on - strand, within GFF930 at 107.577 kb on - strand, within GFF930 at 107.578 kb on + strand, within GFF930 at 107.578 kb on + strand, within GFF930 at 107.579 kb on - strand, within GFF930 at 107.579 kb on - strand, within GFF930 at 107.761 kb on + strand, within GFF930 at 107.762 kb on - strand, within GFF930 at 107.786 kb on + strand, within GFF930 at 107.963 kb on - strand, within GFF930 at 107.969 kb on + strand, within GFF930 at 107.969 kb on + strand, within GFF930 at 107.969 kb on + strand, within GFF930 at 108.194 kb on + strand at 108.230 kb on + strand at 108.261 kb on + strand at 108.573 kb on + strand, within GFF931 at 108.573 kb on + strand, within GFF931 at 108.685 kb on - strand, within GFF931 at 108.685 kb on - strand, within GFF931 at 108.953 kb on + strand at 108.954 kb on - strand at 108.992 kb on + strand, within GFF932 at 109.119 kb on + strand at 109.135 kb on + strand at 109.161 kb on - strand at 109.161 kb on - strand at 109.239 kb on - strand at 109.248 kb on - strand at 109.863 kb on + strand, within GFF933 at 109.924 kb on + strand, within GFF933 at 109.924 kb on + strand, within GFF933
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with phi19h1_phage 15 MOI remove 107,565 + GFF930 0.26 +0.8 107,577 - GFF930 0.27 +0.8 107,577 - GFF930 0.27 -0.5 107,577 - GFF930 0.27 +0.2 107,578 + GFF930 0.27 -0.2 107,578 + GFF930 0.27 +0.6 107,579 - GFF930 0.27 -0.0 107,579 - GFF930 0.27 -1.2 107,761 + GFF930 0.47 -0.3 107,762 - GFF930 0.47 -0.3 107,786 + GFF930 0.50 +1.3 107,963 - GFF930 0.68 -0.3 107,969 + GFF930 0.69 -0.3 107,969 + GFF930 0.69 +0.2 107,969 + GFF930 0.69 +0.1 108,194 + +0.5 108,230 + -0.1 108,261 + -0.0 108,573 + GFF931 0.18 +0.0 108,573 + GFF931 0.18 -0.5 108,685 - GFF931 0.41 -0.4 108,685 - GFF931 0.41 -0.8 108,953 + -0.6 108,954 - -0.6 108,992 + GFF932 0.20 -0.1 109,119 + -0.4 109,135 + -0.3 109,161 - +0.3 109,161 - +1.4 109,239 - -0.6 109,248 - +0.1 109,863 + GFF933 0.50 +0.6 109,924 + GFF933 0.54 -0.5 109,924 + GFF933 0.54 -0.1
Or see this region's nucleotide sequence